ID   CNRY_CUPMC              Reviewed;          95 AA.
AC   P56621; Q5NUX0; Q9L3G1;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 2.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Nickel and cobalt resistance protein CnrY;
GN   Name=cnrY; Synonyms=ORF0b; OrderedLocusNames=Rmet_6205; ORFNames=RMe0088;
OS   Cupriavidus metallidurans (strain ATCC 43123 / DSM 2839 / NBRC 102507 /
OS   CH34) (Ralstonia metallidurans).
OG   Plasmid pMOL28.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=266264;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8380802; DOI=10.1128/jb.175.3.767-778.1993;
RA   Liesegang H., Lemke K., Siddiqui R.A., Schlegel H.-G.;
RT   "Characterization of the inducible nickel and cobalt resistance determinant
RT   cnr from pMOL28 of Alcaligenes eutrophus CH34.";
RL   J. Bacteriol. 175:767-778(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   van der Lelie D., Monchy S., Taghavi S., McCorkle S., Dunn J.,
RA   Benotmane M., Vallaeys T., Lapidus A., Mergeay M.;
RT   "Sequence and features of the Ralstonia metallidurans CH34 heavy metal
RT   plasmids pMOL28 and pMOL30.";
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43123 / DSM 2839 / NBRC 102507 / CH34;
RX   PubMed=20463976; DOI=10.1371/journal.pone.0010433;
RA   Janssen P.J., Van Houdt R., Moors H., Monsieurs P., Morin N., Michaux A.,
RA   Benotmane M.A., Leys N., Vallaeys T., Lapidus A., Monchy S., Medigue C.,
RA   Taghavi S., McCorkle S., Dunn J., van der Lelie D., Mergeay M.;
RT   "The complete genome sequence of Cupriavidus metallidurans strain CH34, a
RT   master survivalist in harsh and anthropogenic environments.";
RL   PLoS ONE 5:E10433-E10433(2010).
RN   [4]
RP   CHARACTERIZATION, INDUCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=10671463; DOI=10.1128/jb.182.5.1390-1398.2000;
RA   Grass G., Grosse C., Nies D.H.;
RT   "Regulation of the cnr cobalt and nickel resistance determinant from
RT   Ralstonia sp. strain CH34.";
RL   J. Bacteriol. 182:1390-1398(2000).
RN   [5]
RP   CHARACTERIZATION, AND INDUCTION.
RX   PubMed=10671464; DOI=10.1128/jb.182.5.1399-1409.2000;
RA   Tibazarwa C., Wuertz S., Mergeay M., Wyns L., van der Lelie D.;
RT   "Regulation of the cnr cobalt and nickel resistance determinant of
RT   Ralstonia eutropha (Alcaligenes eutrophus) CH34.";
RL   J. Bacteriol. 182:1399-1409(2000).
CC   -!- FUNCTION: Nickel and cobalt resistance proteins CnrA, CnrB, CnrC CnrH
CC       and CnrR may be involved in the regulation of CNR.
CC   -!- FUNCTION: CnrH alone is able to activate cnr expression, and both CnrY
CC       and CrnX are needed for nickel induction of CnrH (PubMed:10671463). In
CC       the absence of wild-type CnrY (due either to a frameshift,
CC       PubMed:10671463 or absence of the transcript, PubMed:10671464), nickel
CC       and cobalt resistance is constitutive, indicating that CrnY may act as
CC       a repressor (PubMed:10671463) or an anti-sigma factor
CC       (PubMed:10671464). {ECO:0000269|PubMed:10671463,
CC       ECO:0000269|PubMed:10671464}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:10671463}; Single-pass membrane protein
CC       {ECO:0000269|PubMed:10671463}.
CC   -!- INDUCTION: By nickel and cobalt. {ECO:0000269|PubMed:10671463,
CC       ECO:0000269|PubMed:10671464}.
CC   -!- SIMILARITY: To A.xylosoxydans NccY. {ECO:0000305}.
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DR   EMBL; M91650; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AJ276513; CAB82448.1; -; Genomic_DNA.
DR   EMBL; X90708; CAI30232.1; -; Genomic_DNA.
DR   EMBL; CP000355; ABF13064.1; -; Genomic_DNA.
DR   PIR; B47056; B47056.
DR   RefSeq; WP_011239971.1; NC_007972.2.
DR   RefSeq; YP_161710.1; NC_006525.1.
DR   PDB; 4CXF; X-ray; 1.75 A; B=1-95.
DR   PDBsum; 4CXF; -.
DR   AlphaFoldDB; P56621; -.
DR   SMR; P56621; -.
DR   EnsemblBacteria; ABF13064; ABF13064; Rmet_6205.
DR   GeneID; 60825777; -.
DR   KEGG; rme:Rmet_6205; -.
DR   HOGENOM; CLU_2370740_0_0_4; -.
DR   OrthoDB; 2070039at2; -.
DR   Proteomes; UP000002429; Plasmid pMOL28.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   DisProt; DP01181; -.
DR   InterPro; IPR035230; CnrY.
DR   Pfam; PF17524; CnrY; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell inner membrane; Cell membrane; Cobalt; Membrane; Nickel;
KW   Plasmid; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..95
FT                   /note="Nickel and cobalt resistance protein CnrY"
FT                   /id="PRO_0000089978"
FT   TOPO_DOM        1..45
FT                   /note="Cytoplasmic"
FT   TRANSMEM        46..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        69..95
FT                   /note="Periplasmic"
FT   CONFLICT        44
FT                   /note="R -> RR (in Ref. 1; M91650)"
FT                   /evidence="ECO:0000305"
FT   HELIX           4..18
FT                   /evidence="ECO:0007829|PDB:4CXF"
FT   HELIX           24..28
FT                   /evidence="ECO:0007829|PDB:4CXF"
SQ   SEQUENCE   95 AA;  10151 MW;  1DE90E9720849CD4 CRC64;
     MADVEEWLTH ARKVTQEASI GVDVTSIQEC ISAEPAQRVL VARRDAWRAI CCAAFAALVA
     FAAINRVATI MLEKPAPTWV ATPSAASPFG LLIGK