CNST_MOUSE
ID CNST_MOUSE Reviewed; 711 AA.
AC Q8CBC4; Q8BFS5; Q8K053;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Consortin;
GN Name=Cnst;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Amnion, Aorta, Cerebellum, and Vein;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Brain, and Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Lung, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP IDENTIFICATION OF ISOFORM 3, INTERACTION WITH GGA1; GGA2; GJA1; GJB1; GJB2;
RP GJB3; GJB6 AND GJC1, AND MUTAGENESIS OF 558-562.
RX PubMed=19864490; DOI=10.1093/hmg/ddp490;
RA del Castillo F.J., Cohen-Salmon M., Charollais A., Caille D., Lampe P.D.,
RA Chavrier P., Meda P., Petit C.;
RT "Consortin, a trans-Golgi network cargo receptor for the plasma membrane
RT targeting and recycling of connexins.";
RL Hum. Mol. Genet. 19:262-275(2010).
CC -!- FUNCTION: Required for targeting of connexins to the plasma membrane.
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with connexins GJA1/CX43, GJB1/CX32, GJB2/CX26,
CC GJB3/CX31, GJB6/CX30 and GJC1/CX45. Also interacts with GGA1 and GGA2.
CC Does not interact with PANX1. {ECO:0000269|PubMed:19864490}.
CC -!- INTERACTION:
CC Q8CBC4-3; Q8R0H9: Gga1; NbExp=2; IntAct=EBI-2615407, EBI-2616212;
CC Q8CBC4-3; Q6P5E6: Gga2; NbExp=2; IntAct=EBI-2615407, EBI-2616239;
CC Q8CBC4-3; P21994: Gjb2; Xeno; NbExp=2; IntAct=EBI-2615407, EBI-2616119;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}. Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}. Cytoplasmic
CC vesicle, secretory vesicle {ECO:0000250}. Note=Located predominantly in
CC the trans-Golgi network. Probably trafficks between the trans-Golgi
CC network and the cell membrane via the secretory pathway (By
CC similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8CBC4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CBC4-2; Sequence=VSP_025821, VSP_025822;
CC Name=3;
CC IsoId=Q8CBC4-3; Sequence=VSP_038657;
CC -!- SIMILARITY: Belongs to the CNST family. {ECO:0000305}.
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DR EMBL; AK036332; BAC29388.1; -; mRNA.
DR EMBL; AK040666; BAC30660.1; -; mRNA.
DR EMBL; AK040899; BAC30736.1; -; mRNA.
DR EMBL; AK146681; BAE27356.1; -; mRNA.
DR EMBL; BC034107; AAH34107.1; -; mRNA.
DR EMBL; BC054802; AAH54802.1; -; mRNA.
DR CCDS; CCDS15562.1; -. [Q8CBC4-1]
DR RefSeq; NP_666217.2; NM_146105.3. [Q8CBC4-1]
DR AlphaFoldDB; Q8CBC4; -.
DR SMR; Q8CBC4; -.
DR BioGRID; 230548; 1.
DR IntAct; Q8CBC4; 9.
DR STRING; 10090.ENSMUSP00000048205; -.
DR iPTMnet; Q8CBC4; -.
DR PhosphoSitePlus; Q8CBC4; -.
DR SwissPalm; Q8CBC4; -.
DR EPD; Q8CBC4; -.
DR MaxQB; Q8CBC4; -.
DR PaxDb; Q8CBC4; -.
DR PeptideAtlas; Q8CBC4; -.
DR PRIDE; Q8CBC4; -.
DR ProteomicsDB; 283468; -. [Q8CBC4-1]
DR ProteomicsDB; 283469; -. [Q8CBC4-2]
DR ProteomicsDB; 283470; -. [Q8CBC4-3]
DR Antibodypedia; 2026; 18 antibodies from 9 providers.
DR DNASU; 226744; -.
DR Ensembl; ENSMUST00000040706; ENSMUSP00000048205; ENSMUSG00000038949. [Q8CBC4-1]
DR GeneID; 226744; -.
DR KEGG; mmu:226744; -.
DR UCSC; uc007dvm.1; mouse. [Q8CBC4-2]
DR UCSC; uc007dvo.1; mouse. [Q8CBC4-1]
DR CTD; 163882; -.
DR MGI; MGI:2445141; Cnst.
DR VEuPathDB; HostDB:ENSMUSG00000038949; -.
DR eggNOG; ENOG502QSMS; Eukaryota.
DR GeneTree; ENSGT00390000005861; -.
DR HOGENOM; CLU_023107_0_0_1; -.
DR InParanoid; Q8CBC4; -.
DR OMA; PLMQEKI; -.
DR OrthoDB; 1451496at2759; -.
DR PhylomeDB; Q8CBC4; -.
DR TreeFam; TF351172; -.
DR BioGRID-ORCS; 226744; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Cnst; mouse.
DR PRO; PR:Q8CBC4; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q8CBC4; protein.
DR Bgee; ENSMUSG00000038949; Expressed in vestibular membrane of cochlear duct and 219 other tissues.
DR Genevisible; Q8CBC4; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0005802; C:trans-Golgi network; ISS:UniProtKB.
DR GO; GO:0030133; C:transport vesicle; ISS:UniProtKB.
DR GO; GO:0071253; F:connexin binding; IDA:UniProtKB.
DR GO; GO:0019902; F:phosphatase binding; ISS:UniProtKB.
DR GO; GO:0042998; P:positive regulation of Golgi to plasma membrane protein transport; ISS:UniProtKB.
DR InterPro; IPR042318; Consortin.
DR InterPro; IPR028129; Consortin_C.
DR PANTHER; PTHR28581; PTHR28581; 1.
DR Pfam; PF15281; Consortin_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cytoplasmic vesicle; Golgi apparatus;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..711
FT /note="Consortin"
FT /id="PRO_0000288907"
FT TOPO_DOM 1..650
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 651..671
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 672..711
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 1..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 294..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 370..389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 394..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 457..496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..34
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 57..90
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 312..327
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 476..490
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..289
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_038657"
FT VAR_SEQ 128..197
FT /note="DAVPVMQTQNATSQAAGEEEAAGVNANDPPKAPALQPLFSLIRGEVAQMDSR
FT ALPLFLHQVAETYFQEED -> EAFRLSLLSFNKNKHFFWCCSAAAPVAPLCGSCIPFV
FT PAVTVYREITTCLVCFLYRPHHGVLTLPSLRHGGFAL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025821"
FT VAR_SEQ 198..711
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025822"
FT MUTAGEN 558..562
FT /note="DSDLL->NSDAA: Does not affect binding to connexins
FT but results in their intracellular accumulation."
FT /evidence="ECO:0000269|PubMed:19864490"
SQ SEQUENCE 711 AA; 76866 MW; B117F1FA8648E8EC CRC64;
MDDSDPPTYS LQIEPQDGCH PGDSVERRVT RLPSVSDENE NQLAGDGPAG LTTSEGAMGR
ATVSEQDSLN NNESFPSSCE AAPTENAENT PSEGPKDDPP SLGQDQKLPA KRSPRAKKSS
PKSAPPGDAV PVMQTQNATS QAAGEEEAAG VNANDPPKAP ALQPLFSLIR GEVAQMDSRA
LPLFLHQVAE TYFQEEDYEK AMKFIQLERL YHEQLLANLS AIQEQWETKW KAVQPRTVTP
LRNSEKGFNG EDFEQLAKIC TTHQDPLLSK LKTAPVEPSP ERKSLARAIM SEEAVGTEAA
AKEPEIETCP STDPSGDRHE EEPQESSPGC HQMEWQTASP ELPGTAGKDH TEELPSSTNA
TLDLHTQSLE TAGSRSGPAA ASNACKDSSC VPAPPTEDHC GVARDPKVAP PSESVAEQKL
STGDDGALPG LISEGKYSQA HRKELCLPLQ DAFEALPRDQ PHSSEVAEPR QPDVTASDGK
SAQSQAGLET GPESALCGDR KACDVSTLCL EVCMAPEERR DSEDRVSKET EDYLHSLLER
CLKDAEDSLS YEDIQDDDSD LLQDLSPEEA SYSLQEDLPP DESTLSLDDL AKKIEIAEAI
PAEGLVSILK KRNDTVGSHP AQMQQKPAKR RVRFQEIDDN LEQDEVGGGS CILLILLCIA
TVFLSVGGTA LYCTLGNIES PVCTDFADNV DFYYTKLLQG VAGLKHWVYL S
