CNTFR_MOUSE
ID CNTFR_MOUSE Reviewed; 372 AA.
AC O88507; Q80T01;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 2.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Ciliary neurotrophic factor receptor subunit alpha;
DE Short=CNTF receptor subunit alpha;
DE Short=CNTFR-alpha;
DE Flags: Precursor;
GN Name=Cntfr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RA Maeda M., Yaguchi N., Hanyuu C., Nakata Y., Onoda N., Tulin E.E.,
RA Kojima T., Hasegawa M., Kikuchi Y., Nomura H.;
RT "Mouse homolog of human ciliary neurotrophic factor receptor.";
RL Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain, and Olfactory epithelium;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-60 AND ASN-70.
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Brown adipose tissue;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Binds to CNTF. The alpha subunit provides the receptor
CC specificity (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Forms a heterotrimer with LIFR and IL6ST. Interacts with
CC heterodimeric neurotropic cytokine composed of CLCF1/CLC and CRLF1/CLF-
CC 1. Either alone or in complex with the heterodimer CLCF1-CRLF1
CC interacts with SORL1; this interaction may promote internalization and
CC lysosomal degradation. {ECO:0000250|UniProtKB:P26992}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor, GPI-
CC anchor {ECO:0000250}.
CC -!- DOMAIN: The WSXWS motif appears to be necessary for proper protein
CC folding and thereby efficient intracellular transport and cell-surface
CC receptor binding. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the type I cytokine receptor family. Type 3
CC subfamily. {ECO:0000305}.
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DR EMBL; AF068615; AAC25711.1; -; mRNA.
DR EMBL; BC046974; AAH46974.1; -; mRNA.
DR EMBL; BC050928; AAH50928.1; -; mRNA.
DR CCDS; CCDS18067.1; -.
DR RefSeq; NP_001129528.1; NM_001136056.3.
DR RefSeq; NP_057882.2; NM_016673.2.
DR RefSeq; XP_006537660.1; XM_006537597.3.
DR RefSeq; XP_006537661.1; XM_006537598.2.
DR RefSeq; XP_006537662.1; XM_006537599.3.
DR RefSeq; XP_011248218.1; XM_011249916.2.
DR RefSeq; XP_011248219.1; XM_011249917.2.
DR RefSeq; XP_017175430.1; XM_017319941.1.
DR RefSeq; XP_017175431.1; XM_017319942.1.
DR AlphaFoldDB; O88507; -.
DR BMRB; O88507; -.
DR SMR; O88507; -.
DR BioGRID; 198796; 2.
DR IntAct; O88507; 3.
DR MINT; O88507; -.
DR STRING; 10090.ENSMUSP00000100026; -.
DR GlyConnect; 2215; 5 N-Linked glycans (2 sites).
DR GlyGen; O88507; 4 sites, 5 N-linked glycans (2 sites).
DR iPTMnet; O88507; -.
DR PhosphoSitePlus; O88507; -.
DR MaxQB; O88507; -.
DR PaxDb; O88507; -.
DR PRIDE; O88507; -.
DR ProteomicsDB; 283410; -.
DR Antibodypedia; 25488; 334 antibodies from 39 providers.
DR DNASU; 12804; -.
DR Ensembl; ENSMUST00000102961; ENSMUSP00000100026; ENSMUSG00000028444.
DR Ensembl; ENSMUST00000102962; ENSMUSP00000100027; ENSMUSG00000028444.
DR GeneID; 12804; -.
DR KEGG; mmu:12804; -.
DR UCSC; uc008sje.2; mouse.
DR CTD; 1271; -.
DR MGI; MGI:99605; Cntfr.
DR VEuPathDB; HostDB:ENSMUSG00000028444; -.
DR eggNOG; ENOG502QUDK; Eukaryota.
DR GeneTree; ENSGT00940000158864; -.
DR HOGENOM; CLU_047259_0_0_1; -.
DR InParanoid; O88507; -.
DR OMA; KICDTGE; -.
DR OrthoDB; 741136at2759; -.
DR PhylomeDB; O88507; -.
DR TreeFam; TF331210; -.
DR Reactome; R-MMU-6788467; IL-6-type cytokine receptor ligand interactions.
DR SABIO-RK; O88507; -.
DR BioGRID-ORCS; 12804; 1 hit in 73 CRISPR screens.
DR ChiTaRS; Cntfr; mouse.
DR PRO; PR:O88507; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; O88507; protein.
DR Bgee; ENSMUSG00000028444; Expressed in ventricular zone and 163 other tissues.
DR ExpressionAtlas; O88507; baseline and differential.
DR Genevisible; O88507; MM.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016324; C:apical plasma membrane; ISO:MGI.
DR GO; GO:0070110; C:ciliary neurotrophic factor receptor complex; ISO:MGI.
DR GO; GO:0097059; C:CNTFR-CLCF1 complex; ISO:MGI.
DR GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR GO; GO:0004897; F:ciliary neurotrophic factor receptor activity; IGI:MGI.
DR GO; GO:0019955; F:cytokine binding; ISO:MGI.
DR GO; GO:0004896; F:cytokine receptor activity; IBA:GO_Central.
DR GO; GO:0005102; F:signaling receptor binding; ISO:MGI.
DR GO; GO:0003360; P:brainstem development; IMP:MGI.
DR GO; GO:0070120; P:ciliary neurotrophic factor-mediated signaling pathway; IGI:MGI.
DR GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0097049; P:motor neuron apoptotic process; IMP:MGI.
DR GO; GO:2000672; P:negative regulation of motor neuron apoptotic process; IMP:MGI.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IGI:MGI.
DR GO; GO:0007548; P:sex differentiation; IMP:MGI.
DR GO; GO:0060538; P:skeletal muscle organ development; IMP:MGI.
DR GO; GO:0001967; P:suckling behavior; IMP:MGI.
DR CDD; cd00063; FN3; 1.
DR Gene3D; 2.60.40.10; -; 3.
DR InterPro; IPR003961; FN3_dom.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR003530; Hematopoietin_rcpt_L_F3_CS.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR Pfam; PF00041; fn3; 1.
DR SMART; SM00060; FN3; 1.
DR SMART; SM00409; IG; 1.
DR SMART; SM00408; IGc2; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR SUPFAM; SSF49265; SSF49265; 2.
DR PROSITE; PS50853; FN3; 2.
DR PROSITE; PS01354; HEMATOPO_REC_L_F3; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor;
KW Immunoglobulin domain; Lipoprotein; Membrane; Receptor; Reference proteome;
KW Repeat; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..342
FT /note="Ciliary neurotrophic factor receptor subunit alpha"
FT /id="PRO_0000010993"
FT PROPEP 343..372
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000010994"
FT DOMAIN 27..104
FT /note="Ig-like C2-type"
FT DOMAIN 108..205
FT /note="Fibronectin type-III 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT DOMAIN 206..306
FT /note="Fibronectin type-III 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT REGION 301..338
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 290..294
FT /note="WSXWS motif"
FT COMPBIAS 312..330
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 342
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000255"
FT CARBOHYD 60
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 190
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 46..89
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00114"
FT CONFLICT 2
FT /note="A -> T (in Ref. 1; AAC25711)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 372 AA; 40801 MW; 0704AA35CF1BBE12 CRC64;
MAASVPWACC AVLAAAAAAV YTQKHSPQEA PHVQYERLGA DVTLPCGTAS WDAAVTWRVN
GTDLAPDLLN GSQLILRSLE LGHSGLYACF HRDSWHLRHQ VLLHVGLPPR EPVLSCRSNT
YPKGFYCSWH LPTPTYIPNT FNVTVLHGSK IMVCEKDPAL KNRCHIRYMH LFSTIKYKVS
ISVSNALGHN TTAITFDEFT IVKPDPPENV VARPVPSNPR RLEVTWQTPS TWPDPESFPL
KFFLRYRPLI LDQWQHVELS DGTAHTITDA YAGKEYIIQV AAKDNEIGTW SDWSVAAHAT
PWTEEPRHLT TEAQAPETTT STTSSLAPPP TTKICDPGEL GSGGGPSILF LTSVPVTLVL
AAAAATANNL LI
