CNTI_PSEAB
ID CNTI_PSEAB Reviewed; 284 AA.
AC A0A0H2ZHZ4;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 16-SEP-2015, sequence version 1.
DT 03-AUG-2022, entry version 22.
DE RecName: Full=Pseudopaline exporter CntI {ECO:0000305};
GN Name=cntI {ECO:0000303|PubMed:29214991};
GN Synonyms=zrmD {ECO:0000303|PubMed:28898501};
GN OrderedLocusNames=PA14_63910 {ECO:0000312|EMBL:ABJ14218.1};
OS Pseudomonas aeruginosa (strain UCBPP-PA14).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208963;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCBPP-PA14;
RX PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT combinatorial.";
RL Genome Biol. 7:R90.1-R90.14(2006).
RN [2]
RP INDUCTION.
RC STRAIN=UCBPP-PA14;
RX PubMed=28898501; DOI=10.1111/mmi.13834;
RA Mastropasqua M.C., D'Orazio M., Cerasi M., Pacello F., Gismondi A.,
RA Canini A., Canuti L., Consalvo A., Ciavardelli D., Chirullo B.,
RA Pasquali P., Battistoni A.;
RT "Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by
RT a nicotianamine-related metallophore.";
RL Mol. Microbiol. 106:543-561(2017).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=UCBPP-PA14;
RX PubMed=29214991; DOI=10.1038/s41598-017-16765-9;
RA Lhospice S., Gomez N.O., Ouerdane L., Brutesco C., Ghssein G., Hajjar C.,
RA Liratni A., Wang S., Richaud P., Bleves S., Ball G., Borezee-Durant E.,
RA Lobinski R., Pignol D., Arnoux P., Voulhoux R.;
RT "Pseudomonas aeruginosa zinc uptake in chelating environment is primarily
RT mediated by the metallophore pseudopaline.";
RL Sci. Rep. 7:17132-17132(2017).
CC -!- FUNCTION: Transports the metallophore pseudopaline, which is involved
CC in the acquisition of nickel and zinc, and thus enables bacterial
CC growth inside the host, where metal access is limited. Is probably
CC involved in the export of pseudopaline. {ECO:0000269|PubMed:29214991}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:29214991}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Is part of the operon cntOLMI that is negatively regulated
CC by zinc level through the Zur repressor, which leads to transcriptional
CC activation of this operon under zinc depletion.
CC {ECO:0000269|PubMed:28898501, ECO:0000269|PubMed:29214991}.
CC -!- DISRUPTION PHENOTYPE: Mutant shows a large decrease in the
CC extracellular pseudopaline level, with a concomitant increase in the
CC intracellular space. Mutant is unable to grow in airway mucus
CC secretions (AMS) and is impaired in iron accumulation in this media
CC supplemented with iron. {ECO:0000269|PubMed:29214991}.
CC -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
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DR EMBL; CP000438; ABJ14218.1; -; Genomic_DNA.
DR RefSeq; WP_004365418.1; NZ_CP034244.1.
DR AlphaFoldDB; A0A0H2ZHZ4; -.
DR SMR; A0A0H2ZHZ4; -.
DR EnsemblBacteria; ABJ14218; ABJ14218; PA14_63910.
DR KEGG; pau:PA14_63910; -.
DR HOGENOM; CLU_032828_0_1_6; -.
DR OMA; YSYVQIV; -.
DR BioCyc; PAER208963:G1G74-5404-MON; -.
DR Proteomes; UP000000653; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Membrane; Repeat; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..284
FT /note="Pseudopaline exporter CntI"
FT /id="PRO_0000447265"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 8..138
FT /note="EamA 1"
FT /evidence="ECO:0000255"
FT DOMAIN 151..279
FT /note="EamA 2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 284 AA; 30696 MW; F8589AF42175CFE7 CRC64;
MVLDLLKSGV LLAVLASFTF SVMNALVKEA SATLPAAEIV FFRSAIGTLL IYLLMRQAGV
ALSRQGVPML LVRGVMGALY LVCYFYAIAH IPLADASILA HMSPFFVILF SALFLGERIP
RAVYWLLLVV VLGALMIVKP FSYSSYSVYA VVGLLSAVFA AGASVAIRQL SARHHTYEIV
FYFLAVATLV AIPLMWSDFV VPATLREWGL LLAIGVVSLL GQVFLTRAFS HESATIVAVT
RYIGIVFNAG WGWLFWSEVP DALTIAGGVL IVVACIALSR TKKG
