CNTI_PSEAE
ID CNTI_PSEAE Reviewed; 284 AA.
AC Q9HUX6;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Pseudopaline exporter CntI {ECO:0000305};
GN Name=cntI {ECO:0000250|UniProtKB:A0A0H2ZHZ4};
GN Synonyms=zrmD {ECO:0000303|PubMed:28898501};
GN OrderedLocusNames=PA4834 {ECO:0000312|EMBL:AAG08219.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=26446565; DOI=10.1038/srep14644;
RA Gi M., Lee K.M., Kim S.C., Yoon J.H., Yoon S.S., Choi J.Y.;
RT "A novel siderophore system is essential for the growth of Pseudomonas
RT aeruginosa in airway mucus.";
RL Sci. Rep. 5:14644-14644(2015).
RN [3]
RP INDUCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=28898501; DOI=10.1111/mmi.13834;
RA Mastropasqua M.C., D'Orazio M., Cerasi M., Pacello F., Gismondi A.,
RA Canini A., Canuti L., Consalvo A., Ciavardelli D., Chirullo B.,
RA Pasquali P., Battistoni A.;
RT "Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by
RT a nicotianamine-related metallophore.";
RL Mol. Microbiol. 106:543-561(2017).
CC -!- FUNCTION: Transports the metallophore pseudopaline, which is involved
CC in the acquisition of nickel and zinc, and thus enables bacterial
CC growth inside the host, where metal access is limited. Is probably
CC involved in the export of pseudopaline (By similarity). Essential for
CC iron acquisition during the interaction with airway mucus secretions
CC (AMS) (PubMed:26446565). {ECO:0000250|UniProtKB:A0A0H2ZHZ4,
CC ECO:0000269|PubMed:26446565}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000255}.
CC -!- INDUCTION: Is part of the operon cntOLMI that is negatively regulated
CC by zinc level through the Zur repressor, which leads to transcriptional
CC activation of this operon under zinc depletion (PubMed:28898501).
CC Highly induced in response to airway mucus secretions (AMS) treatment
CC (PubMed:26446565). {ECO:0000269|PubMed:26446565,
CC ECO:0000269|PubMed:28898501}.
CC -!- DISRUPTION PHENOTYPE: The deletion mutant is susceptible to treatment
CC with AMS and shows decreased intracellular iron content after
CC incubation with AMS. In vivo virulence of the mutant is attenuated.
CC {ECO:0000269|PubMed:26446565}.
CC -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
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DR EMBL; AE004091; AAG08219.1; -; Genomic_DNA.
DR PIR; F83042; F83042.
DR RefSeq; NP_253521.1; NC_002516.2.
DR RefSeq; WP_003104336.1; NZ_QZGE01000002.1.
DR AlphaFoldDB; Q9HUX6; -.
DR SMR; Q9HUX6; -.
DR STRING; 287.DR97_2184; -.
DR PaxDb; Q9HUX6; -.
DR DNASU; 882351; -.
DR EnsemblBacteria; AAG08219; AAG08219; PA4834.
DR GeneID; 882351; -.
DR KEGG; pae:PA4834; -.
DR PATRIC; fig|208964.12.peg.5065; -.
DR PseudoCAP; PA4834; -.
DR HOGENOM; CLU_032828_0_1_6; -.
DR InParanoid; Q9HUX6; -.
DR OMA; YSYVQIV; -.
DR PhylomeDB; Q9HUX6; -.
DR BioCyc; PAER208964:G1FZ6-4948-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
PE 2: Evidence at transcript level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..284
FT /note="Pseudopaline exporter CntI"
FT /id="PRO_0000447266"
FT TRANSMEM 2..22
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 147..167
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 209..229
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 259..279
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 8..138
FT /note="EamA 1"
FT /evidence="ECO:0000255"
FT DOMAIN 151..279
FT /note="EamA 2"
FT /evidence="ECO:0000255"
SQ SEQUENCE 284 AA; 30723 MW; 45444B393DB8CFF2 CRC64;
MVLDLLKSGV LLAVLASFTF SVMNALVKEA SATLPAAEIV FFRSAIGTLL IYLLMRQAGV
ALSRQGVPML LVRGVMGALY LVCYFYAIAH IPLADASILA HMSPFFVILF SALFLGERIP
RAVYWLLLVV VLGALMIVKP FSYSSYSVYA VVGLLSAVFA AGASVAIRQL SARHHTYEIV
FYFLAVATLV AIPLMWNDFV VPATLREWGL LLAIGVVSLL GQVFLTRAFS HESATIVAVT
RYIGIVFNAG WGWLFWSEVP DALTIAGGVL IVVACIALSR TKKG
