ACL6B_MOUSE
ID ACL6B_MOUSE Reviewed; 426 AA.
AC Q99MR0; Q54A88;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Actin-like protein 6B;
DE AltName: Full=53 kDa BRG1-associated factor B;
DE AltName: Full=Actin-related protein Baf53b;
DE AltName: Full=ArpN-alpha;
DE Short=ArpNa;
DE AltName: Full=BRG1-associated factor 53B;
DE Short=BAF53B;
GN Name=Actl6b; Synonyms=Actl6, Arpna, Baf53b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP INTERACTION WITH SMARCA2, AND INDUCTION.
RC TISSUE=Embryonic carcinoma;
RX PubMed=12437990; DOI=10.1016/s0006-291x(02)02637-2;
RA Kuroda Y., Oma Y., Nishimori K., Ohta T., Harata M.;
RT "Brain-specific expression of the nuclear actin-related protein ArpNalpha
RT and its involvement in mammalian SWI/SNF chromatin remodeling complex.";
RL Biochem. Biophys. Res. Commun. 299:328-334(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/Sv;
RX PubMed=11239002; DOI=10.1093/nar/29.6.1352;
RA Wilson M.D., Riemer C., Martindale D.W., Schnupf P., Boright A.P.,
RA Cheung T.L., Hardy D.M., Schwartz S., Scherer S.W., Tsui L.-C., Miller W.,
RA Koop B.F.;
RT "Comparative analysis of the gene-dense ACHE/TFR2 region on human
RT chromosome 7q22 with the orthologous region on mouse chromosome 5.";
RL Nucleic Acids Res. 29:1352-1365(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION, IDENTIFICATION IN THE NBAF COMPLEX WITH ACTB; ARID1A; SMARCA2;
RP SMARCA4; SMARCB1; SMARCC1; SMARCC2; SMARCD2 AND SMARCE1, DEVELOPMENTAL
RP STAGE, AND TISSUE SPECIFICITY.
RX PubMed=12368262; DOI=10.1101/gad.992102;
RA Olave I., Wang W., Xue Y., Kuo A., Crabtree G.R.;
RT "Identification of a polymorphic, neuron-specific chromatin remodeling
RT complex.";
RL Genes Dev. 16:2509-2517(2002).
RN [6]
RP FUNCTION OF THE NBAF AND NPBAF COMPLEXES, IDENTIFICATION BY MASS
RP SPECTROMETRY, IDENTIFICATION IN THE NBAF COMPLEX, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=17640523; DOI=10.1016/j.neuron.2007.06.019;
RA Lessard J., Wu J.I., Ranish J.A., Wan M., Winslow M.M., Staahl B.T., Wu H.,
RA Aebersold R., Graef I.A., Crabtree G.R.;
RT "An essential switch in subunit composition of a chromatin remodeling
RT complex during neural development.";
RL Neuron 55:201-215(2007).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND IDENTIFICATION IN THE NBAF COMPLEX.
RX PubMed=17920018; DOI=10.1016/j.neuron.2007.08.021;
RA Wu J.I., Lessard J., Olave I.A., Qiu Z., Ghosh A., Graef I.A.,
RA Crabtree G.R.;
RT "Regulation of dendritic development by neuron-specific chromatin
RT remodeling complexes.";
RL Neuron 56:94-108(2007).
RN [8]
RP REVIEW ON SWI/SNF CHROMATIN REMODELING COMPLEXES.
RX PubMed=22952240; DOI=10.1074/jbc.r111.309302;
RA Euskirchen G., Auerbach R.K., Snyder M.;
RT "SWI/SNF chromatin-remodeling factors: multiscale analyses and diverse
RT functions.";
RL J. Biol. Chem. 287:30897-30905(2012).
RN [9]
RP REVIEW ON SWI/SNF CHROMATIN REMODELING COMPLEXES.
RX PubMed=26601204; DOI=10.1126/sciadv.1500447;
RA Kadoch C., Crabtree G.R.;
RT "Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic
RT insights gained from human genomics.";
RL Sci. Adv. 1:E1500447-E1500447(2015).
CC -!- FUNCTION: Involved in transcriptional activation and repression of
CC select genes by chromatin remodeling (alteration of DNA-nucleosome
CC topology). Component of SWI/SNF chromatin remodeling complexes that
CC carry out key enzymatic activities, changing chromatin structure by
CC altering DNA-histone contacts within a nucleosome in an ATP-dependent
CC manner. Belongs to the neuron-specific chromatin remodeling complex
CC (nBAF complex), as such plays a role in remodeling mononucleosomes in
CC an ATP-dependent fashion, and is required for postmitotic neural
CC development and dendritic outgrowth. During neural development a switch
CC from a stem/progenitor to a postmitotic chromatin remodeling mechanism
CC occurs as neurons exit the cell cycle and become committed to their
CC adult state. The transition from proliferating neural stem/progenitor
CC cells to postmitotic neurons requires a switch in subunit composition
CC of the npBAF and nBAF complexes. As neural progenitors exit mitosis and
CC differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A
CC and PHF10/BAF45A, are exchanged for homologous alternative
CC ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-
CC specific complexes (nBAF). The npBAF complex is essential for the self-
CC renewal/proliferative capacity of the multipotent neural stem cells.
CC The nBAF complex along with CREST plays a role regulating the activity
CC of genes essential for dendrite growth. ACTL6B/BAF53B is not essential
CC for assembly of the nBAF complex but is required for targeting the
CC complex and CREST to the promoter of genes essential for dendritic
CC growth. Essential for neuronal maturation and dendrite development (By
CC similarity). {ECO:0000250|UniProtKB:O94805,
CC ECO:0000269|PubMed:12368262, ECO:0000269|PubMed:17640523,
CC ECO:0000269|PubMed:17920018}.
CC -!- SUBUNIT: Component of the multiprotein chromatin-remodeling complexes
CC SWI/SNF: SWI/SNF-A (BAF), SWI/SNF-B (PBAF) and related complexes. The
CC canonical complex contains a catalytic subunit (either
CC SMARCA4/BRG1/BAF190A or SMARCA2/BRM/BAF190B) and at least SMARCE1,
CC ACTL6A/BAF53, SMARCC1/BAF155, SMARCC2/BAF170, and SMARCB1/SNF5/BAF47.
CC Other subunits specific to each of the complexes may also be present
CC permitting several possible combinations developmentally and tissue
CC specific (PubMed:22952240, PubMed:26601204).Component of the BAF
CC complex, which includes at least actin (ACTB), ARID1A/BAF250A,
CC ARID1B/BAF250B, SMARCA2/BRM, SMARCA4/BRG1/BAF190A, ACTL6A/BAF53,
CC ACTL6B/BAF53B, SMARCE1/BAF57, SMARCC1/BAF155, SMARCC2/BAF170,
CC SMARCB1/SNF5/INI1, and one or more SMARCD1/BAF60A, SMARCD2/BAF60B, or
CC SMARCD3/BAF60C (By similarity). Component of neuron-specific chromatin
CC remodeling complex (nBAF complex) composed of at least, ARID1A/BAF250A
CC or ARID1B/BAF250B, SMARCD1/BAF60A or SMARCD2/BAF60B or SMARCD3/BAF60C,
CC SMARCA2/BRM/BAF190B, SMARCA4/BRG1/BAF190A, SMARCB1/BAF47,
CC SMARCC1/BAF155, SMARCE1/BAF57, SMARCC2/BAF170, DPF1/BAF45B,
CC DPF3/BAF45C, ACTL6B/BAF53B and actin (ACTB)(PubMed:12368262,
CC PubMed:17640523, PubMed:17920018). Note that the nBAF complex is
CC polymorphic in regard to the ATPase, SMARCA2 and SMARCA4 occupying
CC mutually exclusive positions (PubMed:12368262). May be a component of
CC the SWI/SNF-B (PBAF) chromatin remodeling complex, at least composed of
CC SMARCA4/BRG1, SMARCB1/BAF47/SNF5, ACTL6A/BAF53A or ACTL6B/BAF53B,
CC SMARCE1/BAF57, SMARCD1/BAF60A, SMARCD2/BAF60B, perhaps SMARCD3/BAF60C,
CC SMARCC1/BAF155, SMARCC2/BAF170, PBRM1/BAF180, ARID2/BAF200 and actin
CC (By similarity). {ECO:0000250|UniProtKB:O94805,
CC ECO:0000269|PubMed:12368262, ECO:0000269|PubMed:17640523,
CC ECO:0000269|PubMed:17920018, ECO:0000303|PubMed:22952240,
CC ECO:0000303|PubMed:26601204}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12437990}.
CC -!- TISSUE SPECIFICITY: Restricted to the brain and peripheral nervous
CC tissue (at protein level). Present in virtually all neurons in the
CC cerebral neocortex (layers II-VI), hippocampus (CA1-CA3 region and
CC dentate gyrus), cerebellum (molecular, granular and Purkinje cell
CC layers), spinal cord (dorsally and ventrally), dorsal root ganglion,
CC retina and the olfactory bulb (mitral and granule cell layers).
CC Expressed specifically in postmitotic neurons (at protein level).
CC {ECO:0000269|PubMed:12368262, ECO:0000269|PubMed:12437990,
CC ECO:0000269|PubMed:17640523}.
CC -!- DEVELOPMENTAL STAGE: Expression begins near the time of neuronal cell
CC type specification. First apparent at 10.5 dpc in the nervous system,
CC with high levels in the telencephalon and less in the diencephalon,
CC mesencephalon and spinal cord. This pattern of expression persists and
CC becomes more defined at 12.5 dpc and later stages. Detected in dorsal
CC root ganglia, but not in neural crest derivatives that give rise to
CC non-neuronal tissues, including great vessels. Expressed in postmitotic
CC cells in the CNS, but not in actively proliferating precursor cells. At
CC protein level, first detected at 12.5 dpc in nervous tissues. In the
CC developing forebrain, cerebellar primordium and spinal cord, strictly
CC expressed in postmitotic neurons. {ECO:0000269|PubMed:12368262,
CC ECO:0000269|PubMed:17640523}.
CC -!- INDUCTION: By retinoic acid of P19 embryonic carcinoma stem cells
CC induced by this treatment to differentiate into neuronal cells.
CC {ECO:0000269|PubMed:12437990}.
CC -!- DISRUPTION PHENOTYPE: Mice have lethal defects in neuronal development,
CC including defects in activity-dependent dendritic outgrowth.
CC {ECO:0000269|PubMed:17920018}.
CC -!- SIMILARITY: Belongs to the actin family. {ECO:0000305}.
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DR EMBL; AB075226; BAB97312.1; -; mRNA.
DR EMBL; AF312033; AAK28829.1; -; Genomic_DNA.
DR EMBL; CH466529; EDL19262.1; -; Genomic_DNA.
DR EMBL; BC049539; AAH49539.1; -; mRNA.
DR CCDS; CCDS19770.1; -.
DR RefSeq; NP_113581.1; NM_031404.4.
DR AlphaFoldDB; Q99MR0; -.
DR SMR; Q99MR0; -.
DR BioGRID; 219970; 3.
DR ComplexPortal; CPX-1236; SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA2 variant.
DR ComplexPortal; CPX-1237; SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA4 variant.
DR ComplexPortal; CPX-1238; SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA2 variant.
DR ComplexPortal; CPX-1239; SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA4 variant.
DR ComplexPortal; CPX-1244; Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA2 variant.
DR ComplexPortal; CPX-1245; Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1A-SMARCA4 variant.
DR ComplexPortal; CPX-1246; Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA2 variant.
DR ComplexPortal; CPX-1247; Muscle cell-specific SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B-ARID1B-SMARCA4 variant.
DR ComplexPortal; CPX-1250; Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B variant.
DR ComplexPortal; CPX-1256; Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant.
DR ComplexPortal; CPX-1257; Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant.
DR ComplexPortal; CPX-1258; Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant.
DR ComplexPortal; CPX-1259; Neuron-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant.
DR ComplexPortal; CPX-1261; Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA2 variant.
DR ComplexPortal; CPX-1262; Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1A-SMARCA4 variant.
DR ComplexPortal; CPX-1263; Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA2 variant.
DR ComplexPortal; CPX-1264; Brain-specific SWI/SNF ATP-dependent chromatin remodeling complex, ARID1B-SMARCA4 variant.
DR ComplexPortal; CPX-4227; GBAF (SWI/SNF) ATP-dependent chromatin remodeling complex, ACTL6B-BICRA-SMARCA2 variant.
DR ComplexPortal; CPX-4228; GBAF (SWI/SNF) ATP-dependent chromatin remodeling complex, ACTL6B-BICRAL-SMARCA2 variant.
DR ComplexPortal; CPX-4229; GBAF (SWI/SNF) ATP-dependent chromatin remodeling complex, ACTL6B-BICRA-SMARCA4 variant.
DR ComplexPortal; CPX-4230; GBAF (SWI/SNF) ATP-dependent chromatin remodeling complex, ACTL6B-BICRAL-SMARCA4 variant.
DR IntAct; Q99MR0; 3.
DR MINT; Q99MR0; -.
DR STRING; 10090.ENSMUSP00000119356; -.
DR PhosphoSitePlus; Q99MR0; -.
DR MaxQB; Q99MR0; -.
DR PaxDb; Q99MR0; -.
DR PeptideAtlas; Q99MR0; -.
DR PRIDE; Q99MR0; -.
DR ProteomicsDB; 285704; -.
DR ABCD; Q99MR0; 1 sequenced antibody.
DR Antibodypedia; 30798; 204 antibodies from 32 providers.
DR DNASU; 83766; -.
DR Ensembl; ENSMUST00000139395; ENSMUSP00000119356; ENSMUSG00000029712.
DR GeneID; 83766; -.
DR KEGG; mmu:83766; -.
DR UCSC; uc009acu.1; mouse.
DR CTD; 51412; -.
DR MGI; MGI:1933548; Actl6b.
DR VEuPathDB; HostDB:ENSMUSG00000029712; -.
DR eggNOG; KOG0679; Eukaryota.
DR GeneTree; ENSGT00940000160860; -.
DR HOGENOM; CLU_027965_6_0_1; -.
DR InParanoid; Q99MR0; -.
DR OMA; MWMSKSE; -.
DR OrthoDB; 649708at2759; -.
DR PhylomeDB; Q99MR0; -.
DR TreeFam; TF312863; -.
DR Reactome; R-MMU-3214858; RMTs methylate histone arginines.
DR Reactome; R-MMU-8939243; RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known.
DR BioGRID-ORCS; 83766; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Actl6b; mouse.
DR PRO; PR:Q99MR0; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q99MR0; protein.
DR Bgee; ENSMUSG00000029712; Expressed in embryonic brain and 108 other tissues.
DR ExpressionAtlas; Q99MR0; baseline and differential.
DR Genevisible; Q99MR0; MM.
DR GO; GO:0140092; C:bBAF complex; IC:ComplexPortal.
DR GO; GO:0035060; C:brahma complex; IC:ComplexPortal.
DR GO; GO:0000785; C:chromatin; IC:ComplexPortal.
DR GO; GO:0140288; C:GBAF complex; IC:ComplexPortal.
DR GO; GO:0000776; C:kinetochore; IC:ComplexPortal.
DR GO; GO:0071565; C:nBAF complex; IDA:UniProtKB.
DR GO; GO:0035267; C:NuA4 histone acetyltransferase complex; IBA:GO_Central.
DR GO; GO:0016363; C:nuclear matrix; IC:ComplexPortal.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0016586; C:RSC-type complex; IC:ComplexPortal.
DR GO; GO:0016514; C:SWI/SNF complex; IDA:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0006325; P:chromatin organization; IC:UniProtKB.
DR GO; GO:0006338; P:chromatin remodeling; ISO:MGI.
DR GO; GO:0016358; P:dendrite development; ISS:UniProtKB.
DR GO; GO:0043967; P:histone H4 acetylation; IBA:GO_Central.
DR GO; GO:0045596; P:negative regulation of cell differentiation; IC:ComplexPortal.
DR GO; GO:0007399; P:nervous system development; IMP:UniProtKB.
DR GO; GO:0042551; P:neuron maturation; ISS:UniProtKB.
DR GO; GO:0045597; P:positive regulation of cell differentiation; IC:ComplexPortal.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IC:ComplexPortal.
DR GO; GO:2000781; P:positive regulation of double-strand break repair; IC:ComplexPortal.
DR GO; GO:0045663; P:positive regulation of myoblast differentiation; IC:ComplexPortal.
DR GO; GO:1902459; P:positive regulation of stem cell population maintenance; IC:ComplexPortal.
DR GO; GO:0045582; P:positive regulation of T cell differentiation; IC:ComplexPortal.
DR GO; GO:0070316; P:regulation of G0 to G1 transition; IC:ComplexPortal.
DR GO; GO:2000045; P:regulation of G1/S transition of mitotic cell cycle; IC:ComplexPortal.
DR GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IC:ComplexPortal.
DR GO; GO:2000819; P:regulation of nucleotide-excision repair; IC:ComplexPortal.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0021510; P:spinal cord development; IEA:Ensembl.
DR InterPro; IPR004000; Actin.
DR InterPro; IPR004001; Actin_CS.
DR InterPro; IPR043129; ATPase_NBD.
DR PANTHER; PTHR11937; PTHR11937; 1.
DR Pfam; PF00022; Actin; 1.
DR PRINTS; PR00190; ACTIN.
DR SMART; SM00268; ACTIN; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00432; ACTINS_2; 1.
PE 1: Evidence at protein level;
KW Chromatin regulator; Neurogenesis; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..426
FT /note="Actin-like protein 6B"
FT /id="PRO_0000089136"
FT REGION 39..82
FT /note="Essential for mediating its function in dendritic
FT development; may contribute to neuronal-specific targeting"
FT /evidence="ECO:0000269|PubMed:17920018"
SQ SEQUENCE 426 AA; 46891 MW; 7B9CD9B70E1304CE CRC64;
MSGGVYGGDE VGALVFDIGS FSVRAGYAGE DCPKADFPTT VGLLAAEEGG GLELEGEKEK
KGKIFHIDTN ALHVPRDGAE VMSPLKNGMI EDWECFRAIL DHTYSKHVKS EPNLHPVLMS
EAPWNTRAKR EKLTELMFEQ YNIPAFFLCK TAVLTAFANG RSTGLVLDSG ATHTTAIPVH
DGYVLQQGIV KSPLAGDFIS MQCRELFQEM AIDIIPPYMI AAKEPVREGA PPNWKKKEKL
PQVSKSWHNY MCNEVIQDFQ ASVLQVSDSP YDEQVAAQMP TVHYEMPNGY NTDYGAERLR
IPEGLFDPSN VKGLSGNTML GVGHVVTTSI GMCDIDIRPG LYGSVIVTGG NTLLQGFTDR
LNRELSQKTP PSMRLKLIAS NSTMERKFSP WIGGSILASL GTFQQMWISK QEYEEGGKQC
VERKCP
