ID   CNTO_PSEAB              Reviewed;         708 AA.
AC   A0A0H2ZI93;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 25.
DE   RecName: Full=Metal-pseudopaline receptor CntO {ECO:0000305};
DE   Flags: Precursor;
GN   Name=cntO {ECO:0000303|PubMed:29214991};
GN   Synonyms=zrmA {ECO:0000303|PubMed:28898501};
GN   OrderedLocusNames=PA14_63960 {ECO:0000312|EMBL:ABJ14221.1};
OS   Pseudomonas aeruginosa (strain UCBPP-PA14).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UCBPP-PA14;
RX   PubMed=17038190; DOI=10.1186/gb-2006-7-10-r90;
RA   Lee D.G., Urbach J.M., Wu G., Liberati N.T., Feinbaum R.L., Miyata S.,
RA   Diggins L.T., He J., Saucier M., Deziel E., Friedman L., Li L., Grills G.,
RA   Montgomery K., Kucherlapati R., Rahme L.G., Ausubel F.M.;
RT   "Genomic analysis reveals that Pseudomonas aeruginosa virulence is
RT   combinatorial.";
RL   Genome Biol. 7:R90.1-R90.14(2006).
RN   [2]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=28898501; DOI=10.1111/mmi.13834;
RA   Mastropasqua M.C., D'Orazio M., Cerasi M., Pacello F., Gismondi A.,
RA   Canini A., Canuti L., Consalvo A., Ciavardelli D., Chirullo B.,
RA   Pasquali P., Battistoni A.;
RT   "Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by
RT   a nicotianamine-related metallophore.";
RL   Mol. Microbiol. 106:543-561(2017).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=UCBPP-PA14;
RX   PubMed=29214991; DOI=10.1038/s41598-017-16765-9;
RA   Lhospice S., Gomez N.O., Ouerdane L., Brutesco C., Ghssein G., Hajjar C.,
RA   Liratni A., Wang S., Richaud P., Bleves S., Ball G., Borezee-Durant E.,
RA   Lobinski R., Pignol D., Arnoux P., Voulhoux R.;
RT   "Pseudomonas aeruginosa zinc uptake in chelating environment is primarily
RT   mediated by the metallophore pseudopaline.";
RL   Sci. Rep. 7:17132-17132(2017).
CC   -!- FUNCTION: Transports the metallophore pseudopaline, which is involved
CC       in the acquisition of nickel and zinc, and thus enables bacterial
CC       growth inside the host, where metal access is limited
CC       (PubMed:29214991). Is probably involved in the import of pseudopaline-
CC       metal complexes (PubMed:28898501, PubMed:29214991).
CC       {ECO:0000269|PubMed:28898501, ECO:0000269|PubMed:29214991}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane
CC       {ECO:0000305|PubMed:29214991}.
CC   -!- INDUCTION: Is part of the operon cntOLMI that is negatively regulated
CC       by zinc level through the Zur repressor, which leads to transcriptional
CC       activation of this operon under zinc depletion.
CC       {ECO:0000269|PubMed:28898501, ECO:0000269|PubMed:29214991}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant does not show growth defect in LB
CC       medium, but it exhibits an evident growth defect after treatment with
CC       airway mucus secretions (AMS). Disruption of the gene alters the
CC       intracellular zinc content in VB-MM medium supplemented with zinc.
CC       Mutation severely affect the ability of P.aeruginosa to cause acute
CC       lung and systemic infections in C57BL/6 mice (PubMed:28898501). Mutant
CC       shows a small decrease in the extracellular content of pseudopaline and
CC       is partially impaired in nickel accumulation (PubMed:29214991).
CC       {ECO:0000269|PubMed:28898501, ECO:0000269|PubMed:29214991}.
CC   -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC       {ECO:0000305}.
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DR   EMBL; CP000438; ABJ14221.1; -; Genomic_DNA.
DR   RefSeq; WP_004365419.1; NZ_CP034244.1.
DR   AlphaFoldDB; A0A0H2ZI93; -.
DR   SMR; A0A0H2ZI93; -.
DR   EnsemblBacteria; ABJ14221; ABJ14221; PA14_63960.
DR   KEGG; pau:PA14_63960; -.
DR   HOGENOM; CLU_008287_9_4_6; -.
DR   OMA; YYERSYN; -.
DR   BioCyc; PAER208963:G1G74-5407-MON; -.
DR   Proteomes; UP000000653; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015344; F:siderophore uptake transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0038023; F:signaling receptor activity; IEA:InterPro.
DR   GO; GO:0015675; P:nickel cation transport; IEA:UniProtKB-KW.
DR   GO; GO:0006829; P:zinc ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.170.130.10; -; 1.
DR   Gene3D; 2.40.170.20; -; 1.
DR   InterPro; IPR012910; Plug_dom.
DR   InterPro; IPR037066; Plug_dom_sf.
DR   InterPro; IPR039423; TonB-dep_rcpt.
DR   InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR   InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR   InterPro; IPR010105; TonB_sidphr_rcpt.
DR   PANTHER; PTHR32552; PTHR32552; 1.
DR   Pfam; PF07715; Plug; 1.
DR   Pfam; PF00593; TonB_dep_Rec; 1.
DR   TIGRFAMs; TIGR01783; TonB-siderophor; 1.
PE   2: Evidence at transcript level;
KW   Cell outer membrane; Ion transport; Membrane; Nickel; Nickel transport;
KW   Receptor; Signal; TonB box; Transmembrane; Transmembrane beta strand;
KW   Transport; Zinc; Zinc transport.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..708
FT                   /note="Metal-pseudopaline receptor CntO"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5002603362"
SQ   SEQUENCE   708 AA;  79058 MW;  C2E73CB9DDA31436 CRC64;
     MRVSVSLVLG VGLGCSSPAL WAETESPAEL EVLTVTAEAE RAEGPVQGYR ANRSASATRT
     DTRIEDIPQA ISVVPRQVLD DLDSARIERA LDFAGGVSRQ NNFGGLTMFE YNVRGFTTSE
     FYRDGFSANR GYMNAPDSAT IERVEILKGP ASSLYGRGDP GGTVNLVTKK PQAERFARLH
     ASAGSWDRYR STLDLNTPLD EEGDLLYRMN LAVEDSKGFR DYADGQRLLV APSFSWQLDP
     DTSLLVEAEV VRNRQVFDRG TVAPHNHLGS LPRSRFFGEP DDGKIDNNNE TLQATLRHHL
     NEQWSLRLAS HYKHGHLDGY ASENSSLAAD GYTLRREYRY RDFEWHDSIT QLDLLGDLHT
     GSIRHQLLMG LEYERYHNDE LILRSIPSRN PYAIDIRRPV YGQPKPPFGR DDRNHEEVDA
     MALNLQDQIE FNEKWRGLLG VRFDRYRQDM NATRLNNGRF RDTSSQQTQR AATPRIGVLY
     QATPEVGLFA NASKSFKPNG GTDMAGKAFD PEEGRGYEAG VKLDLLDGRL GMTLAAFHLK
     KKNVLTADPS NPGYQQTAGE ARSQGFDLQF SGQLTEQLRL IGAYAYIDAE VTKDENIARG
     SRLLNVPKHS GSLMGVYEFR EGWLHGADAG AAVNYVGERA GDSSDSGFEL PAYTTVDLLA
     HYPLASNATL GVNVNNLFDR RYYERSYNNV WVAPGEPRNL TMSLTLNY