CNTO_PSEAE
ID CNTO_PSEAE Reviewed; 708 AA.
AC Q9HUX3;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Metal-pseudopaline receptor CntO {ECO:0000305};
DE Flags: Precursor;
GN Name=cntO {ECO:0000250|UniProtKB:A0A0H2ZI93};
GN Synonyms=zrmA {ECO:0000303|PubMed:28898501};
GN OrderedLocusNames=PA4837 {ECO:0000312|EMBL:AAG08222.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP INDUCTION.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=26446565; DOI=10.1038/srep14644;
RA Gi M., Lee K.M., Kim S.C., Yoon J.H., Yoon S.S., Choi J.Y.;
RT "A novel siderophore system is essential for the growth of Pseudomonas
RT aeruginosa in airway mucus.";
RL Sci. Rep. 5:14644-14644(2015).
RN [3]
RP INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=28898501; DOI=10.1111/mmi.13834;
RA Mastropasqua M.C., D'Orazio M., Cerasi M., Pacello F., Gismondi A.,
RA Canini A., Canuti L., Consalvo A., Ciavardelli D., Chirullo B.,
RA Pasquali P., Battistoni A.;
RT "Growth of Pseudomonas aeruginosa in zinc poor environments is promoted by
RT a nicotianamine-related metallophore.";
RL Mol. Microbiol. 106:543-561(2017).
CC -!- FUNCTION: Transports the metallophore pseudopaline, which is involved
CC in the acquisition of nickel and zinc, and thus enables bacterial
CC growth inside the host, where metal access is limited. Is probably
CC involved in the import of pseudopaline-metal complexes.
CC {ECO:0000250|UniProtKB:A0A0H2ZI93}.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000305}.
CC -!- INDUCTION: Is part of the operon cntOLMI that is negatively regulated
CC by zinc level through the Zur repressor, which leads to transcriptional
CC activation of this operon under zinc depletion (PubMed:28898501).
CC Highly induced in response to airway mucus secretions (AMS) treatment
CC (PubMed:26446565). {ECO:0000269|PubMed:26446565,
CC ECO:0000269|PubMed:28898501}.
CC -!- DISRUPTION PHENOTYPE: Deletion mutant does not show growth defect in LB
CC medium, but it exhibits an evident growth defect after treatment with
CC AMS. {ECO:0000269|PubMed:28898501}.
CC -!- SIMILARITY: Belongs to the TonB-dependent receptor family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG08222.1; -; Genomic_DNA.
DR PIR; A83043; A83043.
DR RefSeq; NP_253524.1; NC_002516.2.
DR RefSeq; WP_010895685.1; NZ_QZGE01000002.1.
DR AlphaFoldDB; Q9HUX3; -.
DR SMR; Q9HUX3; -.
DR STRING; 287.DR97_2187; -.
DR PaxDb; Q9HUX3; -.
DR PRIDE; Q9HUX3; -.
DR EnsemblBacteria; AAG08222; AAG08222; PA4837.
DR GeneID; 878137; -.
DR KEGG; pae:PA4837; -.
DR PATRIC; fig|208964.12.peg.5068; -.
DR PseudoCAP; PA4837; -.
DR HOGENOM; CLU_008287_9_4_6; -.
DR InParanoid; Q9HUX3; -.
DR OMA; YYERSYN; -.
DR PhylomeDB; Q9HUX3; -.
DR BioCyc; PAER208964:G1FZ6-4951-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015344; F:siderophore uptake transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; IEA:InterPro.
DR GO; GO:0015675; P:nickel cation transport; IEA:UniProtKB-KW.
DR GO; GO:0006829; P:zinc ion transport; IEA:UniProtKB-KW.
DR Gene3D; 2.170.130.10; -; 1.
DR Gene3D; 2.40.170.20; -; 1.
DR InterPro; IPR012910; Plug_dom.
DR InterPro; IPR037066; Plug_dom_sf.
DR InterPro; IPR039423; TonB-dep_rcpt.
DR InterPro; IPR000531; TonB-dep_rcpt_b-brl.
DR InterPro; IPR036942; TonB_rcpt_b-brl_sf.
DR InterPro; IPR010105; TonB_sidphr_rcpt.
DR PANTHER; PTHR32552; PTHR32552; 1.
DR Pfam; PF07715; Plug; 1.
DR Pfam; PF00593; TonB_dep_Rec; 1.
DR TIGRFAMs; TIGR01783; TonB-siderophor; 1.
PE 2: Evidence at transcript level;
KW Cell outer membrane; Ion transport; Membrane; Nickel; Nickel transport;
KW Receptor; Reference proteome; Signal; TonB box; Transmembrane;
KW Transmembrane beta strand; Transport; Zinc; Zinc transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..708
FT /note="Metal-pseudopaline receptor CntO"
FT /evidence="ECO:0000255"
FT /id="PRO_5004331124"
SQ SEQUENCE 708 AA; 79064 MW; 08EF5E0E0D639CBB CRC64;
MRVSVSLVLG VGLGCSSPAL WAETESPAEL EVLTVTAEAE RAEGPVQGYR ANRTASATRT
DTRIEDIPQA ISVVPRQVLD DLDSARIERA LDFAGGVSRQ NNFGGLTMFE YNVRGFTTSE
FYRDGFSANR GYMNAPDSAT IERVEILKGP ASSLYGRGDP GGTVNLVTKK PQAERFARLH
ASAGSWDRYR STLDLNTPLD EEGDLLYRMN LAVEDSKGFR DYADGQRLLV APSISWQLDP
DTSLLVEAEV VRNRQVFDRG TVAPHNHLGS LPRSRFFGEP DDGKIDNNNE TLQATLRHHF
NEQWSLRLAS HYKHGHLDGY ASENSSLAAD GYSLRREYRY RDFEWHDSIT QLDLLGDLHT
GSIRHQLLMG LEYERYHNDE LILRSIPSRN PYAIDIRRPV YGQPKPPFGR DDRNHEEVDA
MALNLQDQIE FSEKWRGLLG VRFDRYRQDM NATRLNNGRF RETSSQQTQR AATPRIGVLY
QATPEVGLFA NASKSFKPNG GTDMAGKAFD PEEGRGYEAG VKLDLLDGRL GMTLAAFHLK
KKNVLTADPS NPGYQQTAGE ARSQGFDLQF SGQLTEQLRL IGAYAYIDAE VTKDENIARG
SRLLNVPKHS GSLMGVYEFR EGWLHGADAG AAVNYVGERA GDSSDSGFEL PAYTTVDLLA
RYPLASNATL GVNVNNLFDR RYYERSYNNV WVAPGEPRNL TMSLTLNY
