CNTRB_MOUSE
ID CNTRB_MOUSE Reviewed; 887 AA.
AC Q8CB62; B0QZN4; Q5NCF3;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Centrobin;
DE AltName: Full=Centrosomal BRCA2-interacting protein;
DE AltName: Full=LYST-interacting protein 8;
GN Name=Cntrob; Synonyms=Lip8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Bone;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 87-887 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Required for centriole duplication. Inhibition of centriole
CC duplication leading to defects in cytokinesis (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with LYST. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriole {ECO:0000250}. Note=Centriole-associated,
CC asymmetrically localizes to the daughter centriole. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CB62-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CB62-2; Sequence=VSP_016842;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH58512.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK036710; BAC29545.1; -; mRNA.
DR EMBL; AL645527; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC058512; AAH58512.1; ALT_INIT; mRNA.
DR CCDS; CCDS24888.2; -. [Q8CB62-1]
DR RefSeq; NP_766148.2; NM_172560.3. [Q8CB62-1]
DR AlphaFoldDB; Q8CB62; -.
DR SMR; Q8CB62; -.
DR STRING; 10090.ENSMUSP00000090651; -.
DR iPTMnet; Q8CB62; -.
DR PhosphoSitePlus; Q8CB62; -.
DR EPD; Q8CB62; -.
DR jPOST; Q8CB62; -.
DR MaxQB; Q8CB62; -.
DR PaxDb; Q8CB62; -.
DR PeptideAtlas; Q8CB62; -.
DR PRIDE; Q8CB62; -.
DR ProteomicsDB; 285526; -. [Q8CB62-1]
DR ProteomicsDB; 285527; -. [Q8CB62-2]
DR Antibodypedia; 12323; 212 antibodies from 28 providers.
DR DNASU; 216846; -.
DR Ensembl; ENSMUST00000092973; ENSMUSP00000090651; ENSMUSG00000032782. [Q8CB62-1]
DR GeneID; 216846; -.
DR KEGG; mmu:216846; -.
DR UCSC; uc007jpp.1; mouse. [Q8CB62-1]
DR CTD; 116840; -.
DR MGI; MGI:2443290; Cntrob.
DR VEuPathDB; HostDB:ENSMUSG00000032782; -.
DR eggNOG; ENOG502QRRG; Eukaryota.
DR GeneTree; ENSGT00610000086191; -.
DR HOGENOM; CLU_344733_0_0_1; -.
DR InParanoid; Q8CB62; -.
DR OMA; HSGYQPG; -.
DR OrthoDB; 247531at2759; -.
DR PhylomeDB; Q8CB62; -.
DR TreeFam; TF337444; -.
DR BioGRID-ORCS; 216846; 5 hits in 70 CRISPR screens.
DR PRO; PR:Q8CB62; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8CB62; protein.
DR Bgee; ENSMUSG00000032782; Expressed in skin of snout and 255 other tissues.
DR ExpressionAtlas; Q8CB62; baseline and differential.
DR Genevisible; Q8CB62; MM.
DR GO; GO:0005814; C:centriole; ISS:HGNC-UCL.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0019904; F:protein domain specific binding; ISO:MGI.
DR GO; GO:0007099; P:centriole replication; ISS:HGNC-UCL.
DR GO; GO:0051299; P:centrosome separation; ISS:HGNC-UCL.
DR GO; GO:1902410; P:mitotic cytokinetic process; ISS:HGNC.
DR GO; GO:1902017; P:regulation of cilium assembly; IEA:InterPro.
DR InterPro; IPR038923; Centrobin.
DR PANTHER; PTHR34439; PTHR34439; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell cycle; Cell division; Coiled coil; Cytoplasm;
KW Cytoskeleton; Phosphoprotein; Reference proteome.
FT CHAIN 1..887
FT /note="Centrobin"
FT /id="PRO_0000076241"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 360..887
FT /note="Required for centrosome localization"
FT /evidence="ECO:0000250"
FT REGION 465..486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 566..591
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 636..695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 824..887
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 191..557
FT /evidence="ECO:0000255"
FT COMPBIAS 118..133
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 134..153
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 469..483
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 636..650
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 669..688
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 81
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N137"
FT MOD_RES 782
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8N137"
FT VAR_SEQ 1..225
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_016842"
SQ SEQUENCE 887 AA; 99404 MW; 8E5F99097338EF00 CRC64;
MATAAPSPSS PLRPEDLLSD SSEPPGLNQV SSEVTSQLYT SLHLSRQAEA TARAQLYLAP
TSSPPNEGLD SLAQELSRSL SVGLENNLKK KDGSKHIFEM ESVRGQLQTM LHTSRDTAYR
TGSERREEDS FDSDSTATLL NTRPLQDLSP SSSAPALEEL FPRYTSLRPG PPTNPPDFQG
LRDALDAELT RRKHCERHIQ SLQTRVLELQ QQLAVAVAAD HKKDLMIEQL DKTLARVVEG
WNRHEAERTE VLRGLQEERQ AAELTRSKQQ ETVTRLEQSL SEAMEALSRE QEGARLQQRE
KEALEEERQA LTLRLEVEQQ QCRTLQEERD EARAGQLSEH RKLEALQVAL QEERQAWIKQ
EHQLKERLQA LQEEGQAQLE REKGNSQREA QAAWETQQQF ALLQTEVRRL EGDLDTVRRE
RDALQLEMSL VQARYESQRI QMESELAVQL EQRVTERLAE AQENSLRQAA SLRDHHRKQL
QELSGQHQQE LAAQLAQFKV EMADREERQQ QVAQDYELRL AREQARVRDL KSGNQQLEEQ
RAELVERLQA MLQAHWEEAN QLLSTTLLPP NPQAPLAEPS SPGPLEPEKG ERRTWAMPPM
AVALKPVLQQ SREVKGDVPG APSVLCSTSP DLSLLLGPPF QNQNSFQPLE PKPDVTPPTA
GPFSALEAFT DDHRAERPFP EEDPGSDGDA RLPPASQLEG LKNFLQQLLE TAPQSNGNPS
ADLLLPKAGS RAVSSWEEAP QVPRLPPPVH KTKVPLAMAS SLFRVHGLPS TNLQGSGLST
GSPEKDGLNL VDVSELLRLY QARGWGALPA EDLLLYLKRL EHSGTDGQGE LVPRRNTDSR
LGETTRKEIP SQAVPRRLAS VPKTEKPARK KSGHPGPSMR SRGGIWR
