ACL7A_MOUSE
ID ACL7A_MOUSE Reviewed; 440 AA.
AC Q9QY84; A2AMK4; Q9D9P4; Q9JMI5;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Actin-like protein 7A;
DE AltName: Full=Actin-like-7-alpha;
DE AltName: Full=Testis-specific actin-2;
DE Short=T-actin-2;
GN Name=Actl7a; Synonyms=Tact2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=CD-1; TISSUE=Testis;
RX PubMed=10373328; DOI=10.1006/geno.1999.5848;
RA Chadwick B.P., Mull J., Helbling L.A., Gill S., Leyne M., Robbins C.M.,
RA Pinkett H.W., Makalowska I., Maayan C., Blumenfeld A., Axelrod F.B.,
RA Brownstein M., Gusella J.F., Slaugenhaupt S.A.;
RT "Cloning, mapping, and expression of two novel actin genes, actin-like-7A
RT (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia
RT candidate region on 9q31.";
RL Genomics 58:302-309(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RA Tanaka H., Iguchi N., Carvalho C.E., Nozaki M., Nojima H., Nishimune Y.;
RT "Testis specific actin.";
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=129/Sv; TISSUE=Liver;
RX PubMed=12704725; DOI=10.1002/mrd.10276;
RA Hisano M., Yamada S., Tanaka H., Nishimune Y., Nozaki M.;
RT "Genomic structure and promoter activity of the testis haploid germ cell-
RT specific intronless genes, Tact1 and Tact2.";
RL Mol. Reprod. Dev. 65:148-156(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=12672658; DOI=10.1095/biolreprod.103.015867;
RA Tanaka H., Iguchi N., Egydio de Carvalho C., Tadokoro Y., Yomogida K.,
RA Nishimune Y.;
RT "Novel actin-like proteins T-ACTIN 1 and T-ACTIN 2 are differentially
RT expressed in the cytoplasm and nucleus of mouse haploid germ cells.";
RL Biol. Reprod. 69:475-482(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [10]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=21278383; DOI=10.1074/jbc.m110.171264;
RA Boeda B., Knowles P.P., Briggs D.C., Murray-Rust J., Soriano E.,
RA Garvalov B.K., McDonald N.Q., Way M.;
RT "Molecular recognition of the Tes LIM2-3 domains by the actin-related
RT protein Arp7A.";
RL J. Biol. Chem. 286:11543-11554(2011).
RN [11]
RP MUTAGENESIS OF ALA-250, AND FUNCTION.
RX PubMed=32923619; DOI=10.1126/sciadv.aaz4796;
RA Xin A., Qu R., Chen G., Zhang L., Chen J., Tao C., Fu J., Tang J., Ru Y.,
RA Chen Y., Peng X., Shi H., Zhang F., Sun X.;
RT "Disruption in ACTL7A causes acrosomal ultrastructural defects in human and
RT mouse sperm as a novel male factor inducing early embryonic arrest.";
RL Sci. Adv. 6:eaaz4796-eaaz4796(2020).
CC -!- FUNCTION: May play an important role in formation and fusion of Golgi-
CC derived vesicles during acrosome biogenesis.
CC {ECO:0000269|PubMed:32923619}.
CC -!- SUBUNIT: Interacts (via N-terminus) with TES (via LIM domain 2).
CC Heterodimer with TES; the heterodimer interacts with ENAH to form a
CC heterotrimer. Interacts with ACTL9. {ECO:0000250|UniProtKB:Q9Y615}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000269|PubMed:21278383}. Golgi apparatus
CC {ECO:0000269|PubMed:21278383}. Cytoplasm {ECO:0000269|PubMed:12672658}.
CC Nucleus {ECO:0000269|PubMed:12672658, ECO:0000269|PubMed:21278383}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000269|PubMed:21278383}. Note=Detected at the Golgi apparatus
CC during acrosome biogenesis (PubMed:21278383). Detected at the
CC subacrosomal layer in round spermatids (PubMed:21278383). Detected in
CC sperm head and tail (PubMed:21278383).
CC -!- TISSUE SPECIFICITY: Detected in testis. Detected at the acrosome of
CC round spermatids (at protein level). Detected in adult and embryonic
CC testis. Detected in developing male germ cells.
CC {ECO:0000269|PubMed:12672658, ECO:0000269|PubMed:12704725,
CC ECO:0000269|PubMed:21278383}.
CC -!- SIMILARITY: Belongs to the actin family. {ECO:0000305}.
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DR EMBL; AF113519; AAF18298.1; -; mRNA.
DR EMBL; AB023063; BAA90822.1; -; mRNA.
DR EMBL; AB079643; BAC07297.1; -; Genomic_DNA.
DR EMBL; AL807762; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466565; EDL02258.1; -; Genomic_DNA.
DR EMBL; AK006631; BAB24681.1; -; mRNA.
DR EMBL; BC049610; AAH49610.1; -; mRNA.
DR CCDS; CCDS18197.1; -.
DR RefSeq; NP_033741.1; NM_009611.3.
DR AlphaFoldDB; Q9QY84; -.
DR SMR; Q9QY84; -.
DR STRING; 10090.ENSMUSP00000092692; -.
DR iPTMnet; Q9QY84; -.
DR PhosphoSitePlus; Q9QY84; -.
DR PaxDb; Q9QY84; -.
DR PRIDE; Q9QY84; -.
DR ProteomicsDB; 285705; -.
DR Antibodypedia; 14911; 161 antibodies from 23 providers.
DR DNASU; 11470; -.
DR Ensembl; ENSMUST00000095079; ENSMUSP00000092692; ENSMUSG00000070979.
DR GeneID; 11470; -.
DR KEGG; mmu:11470; -.
DR UCSC; uc008sxo.2; mouse.
DR CTD; 10881; -.
DR MGI; MGI:1343051; Actl7a.
DR VEuPathDB; HostDB:ENSMUSG00000070979; -.
DR eggNOG; KOG0676; Eukaryota.
DR GeneTree; ENSGT00940000162158; -.
DR HOGENOM; CLU_027965_0_2_1; -.
DR InParanoid; Q9QY84; -.
DR OMA; MNNSGKH; -.
DR OrthoDB; 964605at2759; -.
DR PhylomeDB; Q9QY84; -.
DR TreeFam; TF354237; -.
DR BioGRID-ORCS; 11470; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Actl7a; mouse.
DR PRO; PR:Q9QY84; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9QY84; protein.
DR Bgee; ENSMUSG00000070979; Expressed in spermatid and 5 other tissues.
DR Genevisible; Q9QY84; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IMP:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005869; C:dynactin complex; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0001673; C:male germ cell nucleus; IDA:MGI.
DR GO; GO:0031514; C:motile cilium; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR GO; GO:0001675; P:acrosome assembly; IMP:UniProtKB.
DR GO; GO:0009566; P:fertilization; IMP:UniProtKB.
DR GO; GO:0007338; P:single fertilization; IEA:UniProtKB-KW.
DR InterPro; IPR004000; Actin.
DR InterPro; IPR027679; ACTL7A.
DR InterPro; IPR031769; ACTL7A_N.
DR InterPro; IPR043129; ATPase_NBD.
DR PANTHER; PTHR11937; PTHR11937; 1.
DR PANTHER; PTHR11937:SF167; PTHR11937:SF167; 1.
DR Pfam; PF00022; Actin; 1.
DR Pfam; PF16840; ACTL7A_N; 1.
DR PRINTS; PR00190; ACTIN.
DR SMART; SM00268; ACTIN; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoplasmic vesicle; Cytoskeleton; Fertilization;
KW Golgi apparatus; Nucleus; Reference proteome.
FT CHAIN 1..440
FT /note="Actin-like protein 7A"
FT /id="PRO_0000089138"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 36..56
FT /note="Required for interaction with TES"
FT /evidence="ECO:0000250|UniProtKB:Q9Y615"
FT MUTAGEN 250
FT /note="A->T: Homozygous knockin male mice are infertile.
FT The spermatogenesis process is normal. However spermatozoa
FT dysplay acrosomal ultrastructural defects. Complete absence
FT of the ACTL7A protein in both testes and sperms. Abnormal
FT distribution of PLCZ1 in sperms."
FT /evidence="ECO:0000269|PubMed:32923619"
FT CONFLICT 176..181
FT /note="DPPLSP -> VTPREC (in Ref. 4; BAB24681)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="V -> D (in Ref. 4; BAB24681)"
FT /evidence="ECO:0000305"
FT CONFLICT 290
FT /note="D -> T (in Ref. 4; BAB24681)"
FT /evidence="ECO:0000305"
FT CONFLICT 328
FT /note="F -> S (in Ref. 2; BAA90822)"
FT /evidence="ECO:0000305"
FT CONFLICT 373
FT /note="R -> G (in Ref. 4; BAB24681)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 440 AA; 49451 MW; 1909838FB9D8DEC6 CRC64;
MSLDGVWAPQ TANIGDGPAK KASDQASMQT QVLQTASLKD GPAKRAVWVR RDNAETEDPV
KSTMSKDRPR LEVTKAVVVD LGTGFCKCGF AGLPKPTHKI STTVGKPYME TAKTGDNRKE
TFVGHELFNP DIHLKLVNPL RHGIIVDWDT VQDIWEYLFR QEMKIAPEEH AVLVSDPPLS
PHTNREKYAE MLFETFNTPA MHIAYQSRLS MYSYGRTSGL VVEVGHGVSY VVPIYEGYPL
PSITGRLDYA GSDLTTYLMN LMNNSGKHFS EDHLGIVEDI KTRCCFVALD PIEEKKIPAP
EHEIHYTLPD GKEIRLGQER FLCSEMFFKP SLIKSMQLGL HTQTVSCLNK CDIALKRDLM
GNILLCGGST MLRGFPNRLQ KELSSMCPND TPQVNVLPER DTAVWTGGSI LASLQGFQPL
WVHRLEYEEH GPFFLYRRCF
