2AB2C_ARATH
ID 2AB2C_ARATH Reviewed; 536 AA.
AC Q8VZQ4;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Probable serine/threonine protein phosphatase 2A regulatory subunit B''gamma;
DE Short=AtB''gamma;
DE AltName: Full=Serine/threonine protein phosphatase 2A regulatory subunit B''beta 2 isoform;
DE Short=AtB''beta 2;
DE Short=PP2A, B'' subunit, beta 2 isoform;
GN Name=B''GAMMA; OrderedLocusNames=At5g28900; ORFNames=F7P1.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=15610358; DOI=10.1111/j.1365-313x.2004.02281.x;
RA Koroleva O.A., Tomlinson M.L., Leader D., Shaw P., Doonan J.H.;
RT "High-throughput protein localization in Arabidopsis using Agrobacterium-
RT mediated transient expression of GFP-ORF fusions.";
RL Plant J. 41:162-174(2005).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=21478440; DOI=10.1105/tpc.110.074278;
RA Leivar P., Antolin-Llovera M., Ferrero S., Closa M., Arro M., Ferrer A.,
RA Boronat A., Campos N.;
RT "Multilevel control of Arabidopsis 3-hydroxy-3-methylglutaryl coenzyme A
RT reductase by protein phosphatase 2A.";
RL Plant Cell 23:1494-1511(2011).
CC -!- FUNCTION: Probable regulatory subunit of type 2A protein phosphatase.
CC {ECO:0000250}.
CC -!- SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed
CC of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant
CC regulatory subunit (PR65 or subunit A), that associates with a variety
CC of regulatory subunits. Proteins that associate with the core dimer
CC include three families of regulatory subunits B (the R2/B/PR55/B55,
CC R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) and cell signaling
CC molecules (By similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q8VZQ4; O80920: PYL4; NbExp=3; IntAct=EBI-4428781, EBI-2349683;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15610358}. Nucleus
CC {ECO:0000269|PubMed:15610358}.
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DR EMBL; AF272706; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED93848.1; -; Genomic_DNA.
DR EMBL; AY063937; AAL36293.1; -; mRNA.
DR EMBL; AY096482; AAM20122.1; -; mRNA.
DR RefSeq; NP_198242.1; NM_122774.4.
DR AlphaFoldDB; Q8VZQ4; -.
DR SMR; Q8VZQ4; -.
DR BioGRID; 18285; 16.
DR IntAct; Q8VZQ4; 17.
DR STRING; 3702.AT5G28900.1; -.
DR iPTMnet; Q8VZQ4; -.
DR PaxDb; Q8VZQ4; -.
DR PRIDE; Q8VZQ4; -.
DR ProMEX; Q8VZQ4; -.
DR DNASU; 833013; -.
DR EnsemblPlants; AT5G28900.1; AT5G28900.1; AT5G28900.
DR GeneID; 833013; -.
DR Gramene; AT5G28900.1; AT5G28900.1; AT5G28900.
DR KEGG; ath:AT5G28900; -.
DR Araport; AT5G28900; -.
DR TAIR; locus:2150471; AT5G28900.
DR eggNOG; KOG2562; Eukaryota.
DR HOGENOM; CLU_019589_3_0_1; -.
DR InParanoid; Q8VZQ4; -.
DR OMA; KPQDEAY; -.
DR OrthoDB; 255081at2759; -.
DR PhylomeDB; Q8VZQ4; -.
DR PRO; PR:Q8VZQ4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR Genevisible; Q8VZQ4; AT.
DR GO; GO:0005737; C:cytoplasm; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0000159; C:protein phosphatase type 2A complex; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central.
DR GO; GO:0006470; P:protein dephosphorylation; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR041534; EF-hand_13.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF17958; EF-hand_13; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SUPFAM; SSF47473; SSF47473; 2.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 3.
PE 1: Evidence at protein level;
KW Calcium; Cytoplasm; Metal-binding; Nucleus; Reference proteome; Repeat.
FT CHAIN 1..536
FT /note="Probable serine/threonine protein phosphatase 2A
FT regulatory subunit B''gamma"
FT /id="PRO_0000422789"
FT DOMAIN 174..209
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 261..296
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 387..422
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 69..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 517..536
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..108
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 400
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 402
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 404
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 411
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
SQ SEQUENCE 536 AA; 62019 MW; 43713D3FD2A58F32 CRC64;
MVDTVIPGDM ACLDADLLQL QEMSSFVLNS KPGFTQKLFD QWLSLPEAQR QVGSLLKDAV
AGAPINVTGS ASGSNSATIP SMFPAGSAPP LSPRSCGSPR TTKQRAPSNL GSTLKVVNEP
VKEPIPQFYF QNGRPPPSEI KEQCMFRINH FFYGHMDGLQ IQEFKLVTRE ICKLPSFFST
SLFRKIDLNN TGFVTRDAFI DFWVNGNMLI MDTTTQIFKI LKQKDQSFIV KDDFKPLLKE
LLATHPGLEF LQSTPEFQER YAETVTYRIF YYINRSGNGR ITFRELKRGN LIDAMLHADE
EEDINKVLRY FSYEHFYVIY CKFWELDTDH DFLIDKENLM RYGNHALTYR IVDRIFSQVA
RKFTNKVEGK MGYEDFVYFI LAEEDKSSVP SLEYWFKCID LDANGIITRN EMQFFYEEQL
HRMECMAQEA VLFEDILCQM IDMIGPENES HITLHDLKGS KLSGNVFNIL FNLNKFMAFE
TRDPFLIRQE RENPTLTDWD RFAHREYIRL SMEEDVEDAS NGSAEVWDES SLEAPF
