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CO4A4_HUMAN
ID   CO4A4_HUMAN             Reviewed;        1690 AA.
AC   P53420; A8MTZ1; Q53RW9; Q53S42; Q53WR1;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 3.
DT   03-AUG-2022, entry version 215.
DE   RecName: Full=Collagen alpha-4(IV) chain;
DE   Flags: Precursor;
GN   Name=COL4A4;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND VARIANTS LEU-1004;
RP   MET-1327 AND PRO-1403.
RC   TISSUE=Kidney;
RX   PubMed=7523402; DOI=10.1016/s0021-9258(18)47174-8;
RA   Leinonen A., Mariyama M., Mochizuki T., Tryggvason K., Reeders S.T.;
RT   "Complete primary structure of the human type IV collagen alpha 4(IV)
RT   chain. Comparison with structure and expression of the other alpha (IV)
RT   chains.";
RL   J. Biol. Chem. 269:26172-26177(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], INVOLVEMENT IN ATS2, VARIANTS ATS2
RP   VAL-1030 AND LEU-1572, AND VARIANTS ALA-545; GLN-570; THR-931; LEU-1004;
RP   MET-1327; SER-1402 AND PRO-1403.
RX   PubMed=9792860; DOI=10.1086/302106;
RA   Boye E., Mollet G., Forestier L., Cohen-Solal L., Heidet L., Cochat P.,
RA   Gruenfeld J.-P., Palcoux J.-B., Gubler M.-C., Antignac C.;
RT   "Determination of the genomic structure of the COL4A4 gene and of novel
RT   mutations causing autosomal recessive Alport syndrome.";
RL   Am. J. Hum. Genet. 63:1329-1340(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
RX   PubMed=9537506; DOI=10.1016/s0014-5793(98)00128-8;
RA   Momota R., Sugimoto M., Oohashi T., Kigasawa K., Yoshioka H., Ninomiya Y.;
RT   "Two genes, COL4A3 and COL4A4 coding for the human alpha3(IV) and
RT   alpha4(IV) collagen chains are arranged head-to-head on chromosome 2q36.";
RL   FEBS Lett. 424:11-16(1998).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1219-1690, AND VARIANTS MET-1327 AND
RP   PRO-1403.
RC   TISSUE=Eye;
RX   PubMed=8365481; DOI=10.1016/0014-5793(93)80256-t;
RA   Sugimoto M., Oohashi T., Yoshioka H., Matsuo N., Ninomiya Y.;
RT   "cDNA isolation and partial gene structure of the human alpha 4(IV)
RT   collagen chain.";
RL   FEBS Lett. 330:122-128(1993).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1407-1507.
RX   PubMed=1429714; DOI=10.1016/s0021-9258(18)35902-7;
RA   Kamagata Y., Mattei M.-G., Ninomiya Y.;
RT   "Isolation and sequencing of cDNAs and genomic DNAs encoding the alpha 4
RT   chain of basement membrane collagen type IV and assignment of the gene to
RT   the distal long arm of human chromosome 2.";
RL   J. Biol. Chem. 267:23753-23758(1992).
RN   [7]
RP   TISSUE SPECIFICITY.
RX   PubMed=8083201; DOI=10.1016/s0021-9258(17)31612-5;
RA   Mariyama M., Leinonen A., Mochizuki T., Tryggvason K., Reeders S.T.;
RT   "Complete primary structure of the human alpha 3(IV) collagen chain.
RT   Coexpression of the alpha 3(IV) and alpha 4(IV) collagen chains in human
RT   tissues.";
RL   J. Biol. Chem. 269:23013-23017(1994).
RN   [8]
RP   HEXAMERIZATION.
RX   PubMed=12193605; DOI=10.1074/jbc.m207769200;
RA   Borza D.B., Bondar O., Todd P., Sundaramoorthy M., Sado Y., Ninomiya Y.,
RA   Hudson B.G.;
RT   "Quaternary organization of the goodpasture autoantigen, the alpha 3(IV)
RT   collagen chain. Sequestration of two cryptic autoepitopes by intrapromoter
RT   interactions with the alpha4 and alpha5 NC1 domains.";
RL   J. Biol. Chem. 277:40075-40083(2002).
RN   [9]
RP   TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=29777959; DOI=10.1016/j.actbio.2018.05.023;
RA   Felemban M., Dorgau B., Hunt N.C., Hallam D., Zerti D., Bauer R., Ding Y.,
RA   Collin J., Steel D., Krasnogor N., Al-Aama J., Lindsay S., Mellough C.,
RA   Lako M.;
RT   "Extracellular matrix component expression in human pluripotent stem cell-
RT   derived retinal organoids recapitulates retinogenesis in vivo and reveals
RT   an important role for IMPG1 and CD44 in the development of photoreceptors
RT   and interphotoreceptor matrix.";
RL   Acta Biomater. 74:207-221(2018).
RN   [10]
RP   REVIEW ON VARIANTS.
RX   PubMed=9195222;
RX   DOI=10.1002/(sici)1098-1004(1997)9:6<477::aid-humu1>3.0.co;2-#;
RA   Lemmink H.H., Schroeder C.H., Monnens L.A.H., Smeets H.J.M.;
RT   "The clinical spectrum of type IV collagen mutations.";
RL   Hum. Mutat. 9:477-499(1997).
RN   [11]
RP   VARIANT ATS2 SER-1201.
RX   PubMed=7987396; DOI=10.1038/ng0994-77;
RA   Mochizuki T., Lemmink H.H., Mariyama M., Antignac C., Gubler M.-C.,
RA   Pirson Y., Verellen-Dumoulin C., Chan B., Schroeder C.H., Smeets H.J.M.,
RA   Reeders S.T.;
RT   "Identification of mutations in the alpha 3(IV) and alpha 4(IV) collagen
RT   genes in autosomal recessive Alport syndrome.";
RL   Nat. Genet. 8:77-82(1994).
RN   [12]
RP   VARIANT BFH GLU-897.
RX   PubMed=8787673; DOI=10.1172/jci118893;
RA   Lemmink H.H., Nillesen W.N., Mochizuki T., Schroeder C.H., Brunner H.G.,
RA   van Oost B.A., Monnens L.A.H., Smeets H.J.M.;
RT   "Benign familial hematuria due to mutation of the type IV collagen alpha4
RT   gene.";
RL   J. Clin. Invest. 98:1114-1118(1996).
RN   [13]
RP   VARIANT BFH ARG-960, AND VARIANTS THR-6; SER-482 AND ALA-545.
RX   PubMed=11961012; DOI=10.1681/asn.v1351248;
RA   Badenas C., Praga M., Tazon B., Heidet L., Arrondel C., Armengol A.,
RA   Andres A., Morales E., Camacho J.A., Lens X., Davila S., Mila M.,
RA   Antignac C., Darnell A., Torra R.;
RT   "Mutations in the COL4A4 and COL4A3 genes cause familial benign
RT   hematuria.";
RL   J. Am. Soc. Nephrol. 13:1248-1254(2002).
RN   [14]
RP   VARIANTS BFH GLU-116; ARG-960; GLU-999 AND LEU-1132, AND VARIANT SER-482.
RX   PubMed=12631110; DOI=10.1046/j.1523-1755.2003.00780.x;
RA   Buzza M., Dagher H., Wang Y.Y., Wilson D., Babon J.J., Cotton R.G.,
RA   Savige J.;
RT   "Mutations in the COL4A4 gene in thin basement membrane disease.";
RL   Kidney Int. 63:447-453(2003).
CC   -!- FUNCTION: Type IV collagen is the major structural component of
CC       glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork
CC       together with laminins, proteoglycans and entactin/nidogen.
CC   -!- SUBUNIT: There are six type IV collagen isoforms, alpha 1(IV)-alpha
CC       6(IV), each of which can form a triple helix structure with 2 other
CC       chains to generate type IV collagen network. The alpha 3(IV) chain
CC       forms a triple helical protomer with alpha 4(IV) and alpha 5(IV); this
CC       triple helical structure dimerizes through NC1-NC1 domain interactions
CC       such that the alpha 3(IV), alpha 4(IV) and alpha 5(IV) chains of one
CC       protomer connect with the alpha 5(IV), alpha 4(IV) and alpha 3(IV)
CC       chains of the opposite protomer, respectively. Associates with LAMB2 at
CC       the neuromuscular junction and in GBM (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC       matrix, basement membrane {ECO:0000255|PROSITE-ProRule:PRU00736}.
CC       Note=Colocalizes with COL4A4 and COL4A5 in GBM, tubular basement
CC       membrane (TBM) and synaptic basal lamina (BL). {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in Bruch's membrane, outer plexiform
CC       layer, inner nuclear layer, inner plexiform layer, ganglion cell layer,
CC       inner limiting membrane and around the blood vessels of the retina (at
CC       protein level) (PubMed:29777959). Alpha 3 and alpha 4 type IV collagens
CC       are colocalized and present in kidney, eye, basement membranes of lens
CC       capsule, cochlea, lung, skeletal muscle, aorta, synaptic fibers, fetal
CC       kidney and fetal lung. PubMed:8083201 reports similar levels of
CC       expression of alpha 3 and alpha 4 type IV collagens in kidney, but
CC       PubMed:7523402 reports that in kidney levels of alpha 3 type IV
CC       collagen are significantly lower than those of alpha 4 type IV
CC       collagen. Highest levels of expression of alpha 4 type IV collagen are
CC       detected in kidney, calvaria, neuroretina and cardiac muscle. Lower
CC       levels of expression are observed in brain, lung and thymus, and no
CC       expression is detected in choroid plexus, liver, adrenal, pancreas,
CC       ileum or skin. {ECO:0000269|PubMed:29777959,
CC       ECO:0000269|PubMed:7523402, ECO:0000269|PubMed:8083201}.
CC   -!- DEVELOPMENTAL STAGE: At 6 weeks post-conception (WPC) expressed in the
CC       hyaloid artery and lens capsule (at protein level) (PubMed:29777959).
CC       Expressed between 6 and 19 WPC in the choroid and Bruch's membrane with
CC       faint expression in the retinal pigment epithelium, outer neuroblastic
CC       zone, inner plexiform layer, and the inner neuroblastic zone (at
CC       protein level) (PubMed:29777959). {ECO:0000269|PubMed:29777959}.
CC   -!- DOMAIN: Alpha chains of type IV collagen have a non-collagenous domain
CC       (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats
CC       in the long central triple-helical domain (which may cause flexibility
CC       in the triple helix), and a short N-terminal triple-helical 7S domain.
CC   -!- PTM: Prolines at the third position of the tripeptide repeating unit
CC       (G-X-Y) are hydroxylated in some or all of the chains.
CC   -!- PTM: Type IV collagens contain numerous cysteine residues which are
CC       involved in inter- and intramolecular disulfide bonding. 12 of these,
CC       located in the NC1 domain, are conserved in all known type IV
CC       collagens.
CC   -!- PTM: The trimeric structure of the NC1 domains is stabilized by
CC       covalent bonds between Lys and Met residues. {ECO:0000250}.
CC   -!- DISEASE: Alport syndrome 2, autosomal recessive (ATS2) [MIM:203780]: A
CC       syndrome characterized by progressive glomerulonephritis, glomerular
CC       basement membrane defects, renal failure, sensorineural deafness and
CC       specific eye abnormalities (lenticonous and macular flecks). The
CC       disorder shows considerable heterogeneity in that families differ in
CC       the age of end-stage renal disease and the occurrence of deafness.
CC       {ECO:0000269|PubMed:7987396, ECO:0000269|PubMed:9792860}. Note=The
CC       disease is caused by variants affecting the gene represented in this
CC       entry.
CC   -!- DISEASE: Hematuria, benign familial (BFH) [MIM:141200]: An autosomal
CC       dominant condition characterized by non-progressive isolated
CC       microscopic hematuria that does not result in renal failure. It is
CC       characterized pathologically by thinning of the glomerular basement
CC       membrane. {ECO:0000269|PubMed:11961012, ECO:0000269|PubMed:12631110,
CC       ECO:0000269|PubMed:8787673}. Note=The disease is caused by variants
CC       affecting the gene represented in this entry.
CC   -!- SIMILARITY: Belongs to the type IV collagen family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00736}.
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DR   EMBL; X81053; CAA56943.1; -; mRNA.
DR   EMBL; Y17397; CAA76763.1; -; Genomic_DNA.
DR   EMBL; Y17398; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17399; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17400; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17401; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17402; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17403; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17404; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17405; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17406; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17407; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17408; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17409; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17410; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17411; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17412; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17413; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17427; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17426; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17414; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17415; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17416; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17417; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17418; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17419; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17420; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17443; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17442; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17441; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17440; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17439; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17438; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17437; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17436; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17435; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17434; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17433; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17432; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17431; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17430; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17429; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17428; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17421; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17422; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17423; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17424; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; Y17425; CAA76763.1; JOINED; Genomic_DNA.
DR   EMBL; AC073149; AAY24061.1; -; Genomic_DNA.
DR   EMBL; AC079235; AAY14670.1; -; Genomic_DNA.
DR   EMBL; AB008496; BAA25065.1; -; Genomic_DNA.
DR   EMBL; D17391; BAA04214.1; -; mRNA.
DR   CCDS; CCDS42828.1; -.
DR   PIR; A55360; CGHU1B.
DR   RefSeq; NP_000083.3; NM_000092.4.
DR   RefSeq; XP_005246338.1; XM_005246281.3.
DR   PDB; 5NB1; X-ray; 2.82 A; A/B/C/D/E/F=1461-1690.
DR   PDB; 6WKU; X-ray; 1.76 A; A=1465-1687.
DR   PDBsum; 5NB1; -.
DR   PDBsum; 6WKU; -.
DR   AlphaFoldDB; P53420; -.
DR   SMR; P53420; -.
DR   BioGRID; 107683; 7.
DR   ComplexPortal; CPX-1725; Collagen type IV trimer variant 3.
DR   IntAct; P53420; 5.
DR   MINT; P53420; -.
DR   STRING; 9606.ENSP00000379866; -.
DR   ChEMBL; CHEMBL3988505; -.
DR   DrugBank; DB06356; TRC093.
DR   GlyGen; P53420; 2 sites.
DR   iPTMnet; P53420; -.
DR   PhosphoSitePlus; P53420; -.
DR   BioMuta; COL4A4; -.
DR   DMDM; 259016360; -.
DR   EPD; P53420; -.
DR   jPOST; P53420; -.
DR   MassIVE; P53420; -.
DR   PaxDb; P53420; -.
DR   PeptideAtlas; P53420; -.
DR   PRIDE; P53420; -.
DR   ProteomicsDB; 56583; -.
DR   Antibodypedia; 54339; 138 antibodies from 23 providers.
DR   DNASU; 1286; -.
DR   Ensembl; ENST00000396625.5; ENSP00000379866.3; ENSG00000081052.14.
DR   GeneID; 1286; -.
DR   KEGG; hsa:1286; -.
DR   MANE-Select; ENST00000396625.5; ENSP00000379866.3; NM_000092.5; NP_000083.3.
DR   UCSC; uc061teu.1; human.
DR   CTD; 1286; -.
DR   DisGeNET; 1286; -.
DR   GeneCards; COL4A4; -.
DR   GeneReviews; COL4A4; -.
DR   HGNC; HGNC:2206; COL4A4.
DR   HPA; ENSG00000081052; Low tissue specificity.
DR   MalaCards; COL4A4; -.
DR   MIM; 120131; gene.
DR   MIM; 141200; phenotype.
DR   MIM; 203780; phenotype.
DR   neXtProt; NX_P53420; -.
DR   OpenTargets; ENSG00000081052; -.
DR   Orphanet; 88918; Autosomal dominant Alport syndrome.
DR   Orphanet; 88919; Autosomal recessive Alport syndrome.
DR   Orphanet; 97562; NON RARE IN EUROPE: Benign familial hematuria.
DR   PharmGKB; PA26721; -.
DR   VEuPathDB; HostDB:ENSG00000081052; -.
DR   eggNOG; KOG3544; Eukaryota.
DR   GeneTree; ENSGT00940000153991; -.
DR   HOGENOM; CLU_002023_1_0_1; -.
DR   InParanoid; P53420; -.
DR   OMA; KGFPGCD; -.
DR   OrthoDB; 63831at2759; -.
DR   PhylomeDB; P53420; -.
DR   TreeFam; TF344135; -.
DR   PathwayCommons; P53420; -.
DR   Reactome; R-HSA-1442490; Collagen degradation.
DR   Reactome; R-HSA-1474244; Extracellular matrix organization.
DR   Reactome; R-HSA-1650814; Collagen biosynthesis and modifying enzymes.
DR   Reactome; R-HSA-186797; Signaling by PDGF.
DR   Reactome; R-HSA-2022090; Assembly of collagen fibrils and other multimeric structures.
DR   Reactome; R-HSA-216083; Integrin cell surface interactions.
DR   Reactome; R-HSA-2214320; Anchoring fibril formation.
DR   Reactome; R-HSA-2243919; Crosslinking of collagen fibrils.
DR   Reactome; R-HSA-3000157; Laminin interactions.
DR   Reactome; R-HSA-3000171; Non-integrin membrane-ECM interactions.
DR   Reactome; R-HSA-3000178; ECM proteoglycans.
DR   Reactome; R-HSA-419037; NCAM1 interactions.
DR   Reactome; R-HSA-8948216; Collagen chain trimerization.
DR   SignaLink; P53420; -.
DR   SIGNOR; P53420; -.
DR   BioGRID-ORCS; 1286; 9 hits in 1072 CRISPR screens.
DR   ChiTaRS; COL4A4; human.
DR   GeneWiki; COL4A4; -.
DR   GenomeRNAi; 1286; -.
DR   Pharos; P53420; Tbio.
DR   PRO; PR:P53420; -.
DR   Proteomes; UP000005640; Chromosome 2.
DR   RNAct; P53420; protein.
DR   Bgee; ENSG00000081052; Expressed in renal medulla and 123 other tissues.
DR   ExpressionAtlas; P53420; baseline and differential.
DR   Genevisible; P53420; HS.
DR   GO; GO:0005604; C:basement membrane; IDA:UniProtKB.
DR   GO; GO:0005587; C:collagen type IV trimer; IDA:UniProtKB.
DR   GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR   GO; GO:0005788; C:endoplasmic reticulum lumen; TAS:Reactome.
DR   GO; GO:0031012; C:extracellular matrix; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0005201; F:extracellular matrix structural constituent; IMP:UniProtKB.
DR   GO; GO:0030198; P:extracellular matrix organization; IBA:GO_Central.
DR   GO; GO:0032836; P:glomerular basement membrane development; IMP:UniProtKB.
DR   Gene3D; 2.170.240.10; -; 1.
DR   InterPro; IPR008160; Collagen.
DR   InterPro; IPR001442; Collagen_IV_NC.
DR   InterPro; IPR036954; Collagen_IV_NC_sf.
DR   InterPro; IPR016187; CTDL_fold.
DR   Pfam; PF01413; C4; 2.
DR   Pfam; PF01391; Collagen; 14.
DR   SMART; SM00111; C4; 2.
DR   SUPFAM; SSF56436; SSF56436; 2.
DR   PROSITE; PS51403; NC1_IV; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alport syndrome; Basement membrane; Collagen; Deafness;
KW   Disease variant; Disulfide bond; Extracellular matrix; Glycoprotein;
KW   Hydroxylation; Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..38
FT                   /evidence="ECO:0000255"
FT   CHAIN           39..1690
FT                   /note="Collagen alpha-4(IV) chain"
FT                   /id="PRO_0000005850"
FT   DOMAIN          1465..1690
FT                   /note="Collagen IV NC1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00736"
FT   REGION          39..64
FT                   /note="7S domain"
FT   REGION          61..173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          65..1459
FT                   /note="Triple-helical region"
FT   REGION          187..258
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          369..390
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          405..451
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          469..1457
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           94..96
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   MOTIF           145..147
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   MOTIF           189..191
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   MOTIF           310..312
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   MOTIF           724..726
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   MOTIF           785..787
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   MOTIF           989..991
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   MOTIF           1212..1214
FT                   /note="Cell attachment site"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        63..79
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        588..602
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        614..644
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        678..692
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        877..911
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1115..1129
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1218..1241
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1253..1278
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1295..1312
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1332..1375
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            1206..1207
FT                   /note="Cleavage; by collagenase"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        142
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        669
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        1480..1569
FT                   /note="Or C-1480 with C-1566"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00736"
FT   DISULFID        1513..1566
FT                   /note="Or C-1513 with C-1569"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00736"
FT   DISULFID        1525..1531
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00736"
FT   DISULFID        1588..1686
FT                   /note="Or C-1588 with C-1683"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00736"
FT   DISULFID        1622..1683
FT                   /note="Or C-1622 with C-1686"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00736"
FT   DISULFID        1634..1641
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00736"
FT   VARIANT         6
FT                   /note="I -> T (in dbSNP:rs16823264)"
FT                   /evidence="ECO:0000269|PubMed:11961012"
FT                   /id="VAR_031622"
FT   VARIANT         116
FT                   /note="G -> E (in BFH; dbSNP:rs1553696235)"
FT                   /evidence="ECO:0000269|PubMed:12631110"
FT                   /id="VAR_031623"
FT   VARIANT         441..446
FT                   /note="Missing (in APSAR)"
FT                   /id="VAR_008148"
FT   VARIANT         482
FT                   /note="P -> S (in dbSNP:rs2229814)"
FT                   /evidence="ECO:0000269|PubMed:11961012,
FT                   ECO:0000269|PubMed:12631110"
FT                   /id="VAR_022069"
FT   VARIANT         545
FT                   /note="G -> A (in dbSNP:rs1800516)"
FT                   /evidence="ECO:0000269|PubMed:11961012,
FT                   ECO:0000269|PubMed:9792860"
FT                   /id="VAR_008149"
FT   VARIANT         570
FT                   /note="E -> Q"
FT                   /evidence="ECO:0000269|PubMed:9792860"
FT                   /id="VAR_008150"
FT   VARIANT         594
FT                   /note="E -> G (in dbSNP:rs35998949)"
FT                   /id="VAR_055680"
FT   VARIANT         670
FT                   /note="V -> I (in dbSNP:rs34236495)"
FT                   /id="VAR_055681"
FT   VARIANT         759
FT                   /note="P -> L (in dbSNP:rs36121515)"
FT                   /id="VAR_055682"
FT   VARIANT         897
FT                   /note="G -> E (in BFH; dbSNP:rs121912860)"
FT                   /evidence="ECO:0000269|PubMed:8787673"
FT                   /id="VAR_001912"
FT   VARIANT         931
FT                   /note="A -> T (in dbSNP:rs75875272)"
FT                   /evidence="ECO:0000269|PubMed:9792860"
FT                   /id="VAR_008151"
FT   VARIANT         960
FT                   /note="G -> R (in BFH; dbSNP:rs769783985)"
FT                   /evidence="ECO:0000269|PubMed:11961012,
FT                   ECO:0000269|PubMed:12631110"
FT                   /id="VAR_031624"
FT   VARIANT         999
FT                   /note="G -> E (in BFH; dbSNP:rs13027659)"
FT                   /evidence="ECO:0000269|PubMed:12631110"
FT                   /id="VAR_031625"
FT   VARIANT         1004
FT                   /note="P -> L (in dbSNP:rs1800517)"
FT                   /evidence="ECO:0000269|PubMed:7523402,
FT                   ECO:0000269|PubMed:9792860"
FT                   /id="VAR_008152"
FT   VARIANT         1030
FT                   /note="G -> V (in ATS2; dbSNP:rs772699709)"
FT                   /evidence="ECO:0000269|PubMed:9792860"
FT                   /id="VAR_008153"
FT   VARIANT         1132
FT                   /note="P -> L (in BFH)"
FT                   /evidence="ECO:0000269|PubMed:12631110"
FT                   /id="VAR_031626"
FT   VARIANT         1201
FT                   /note="G -> S (in ATS2; dbSNP:rs121912858)"
FT                   /evidence="ECO:0000269|PubMed:7987396"
FT                   /id="VAR_001913"
FT   VARIANT         1327
FT                   /note="V -> M (in dbSNP:rs2229813)"
FT                   /evidence="ECO:0000269|PubMed:7523402,
FT                   ECO:0000269|PubMed:8365481, ECO:0000269|PubMed:9792860"
FT                   /id="VAR_031627"
FT   VARIANT         1402
FT                   /note="P -> S"
FT                   /evidence="ECO:0000269|PubMed:9792860"
FT                   /id="VAR_008154"
FT   VARIANT         1403
FT                   /note="S -> P (in dbSNP:rs3752895)"
FT                   /evidence="ECO:0000269|PubMed:7523402,
FT                   ECO:0000269|PubMed:8365481, ECO:0000269|PubMed:9792860"
FT                   /id="VAR_031628"
FT   VARIANT         1572
FT                   /note="P -> L (in ATS2; dbSNP:rs121912863)"
FT                   /evidence="ECO:0000269|PubMed:9792860"
FT                   /id="VAR_008155"
FT   CONFLICT        1361..1363
FT                   /note="GPR -> AIS (in Ref. 3; AAY24061)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1659..1660
FT                   /note="LQ -> FE (in Ref. 5; BAA04214)"
FT                   /evidence="ECO:0000305"
FT   STRAND          1465..1471
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1473..1476
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1486..1498
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1501..1504
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   HELIX           1510..1512
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1513..1516
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1522..1526
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   TURN            1527..1529
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1530..1534
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1539..1544
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   HELIX           1556..1562
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1565..1579
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1581..1584
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1592..1605
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1611..1613
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   HELIX           1619..1621
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1622..1625
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1631..1634
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   HELIX           1636..1638
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1640..1642
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1648..1653
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   HELIX           1657..1659
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   TURN            1660..1662
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1668..1670
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   HELIX           1673..1677
FT                   /evidence="ECO:0007829|PDB:6WKU"
FT   STRAND          1682..1687
FT                   /evidence="ECO:0007829|PDB:6WKU"
SQ   SEQUENCE   1690 AA;  164038 MW;  C55711CDF14A57DB CRC64;
     MWSLHIVLMR CSFRLTKSLA TGPWSLILIL FSVQYVYGSG KKYIGPCGGR DCSVCHCVPE
     KGSRGPPGPP GPQGPIGPLG APGPIGLSGE KGMRGDRGPP GAAGDKGDKG PTGVPGFPGL
     DGIPGHPGPP GPRGKPGMSG HNGSRGDPGF PGGRGALGPG GPLGHPGEKG EKGNSVFILG
     AVKGIQGDRG DPGLPGLPGS WGAGGPAGPT GYPGEPGLVG PPGQPGRPGL KGNPGVGVKG
     QMGDPGEVGQ QGSPGPTLLV EPPDFCLYKG EKGIKGIPGM VGLPGPPGRK GESGIGAKGE
     KGIPGFPGPR GDPGSYGSPG FPGLKGELGL VGDPGLFGLI GPKGDPGNRG HPGPPGVLVT
     PPLPLKGPPG DPGFPGRYGE TGDVGPPGPP GLLGRPGEAC AGMIGPPGPQ GFPGLPGLPG
     EAGIPGRPDS APGKPGKPGS PGLPGAPGLQ GLPGSSVIYC SVGNPGPQGI KGKVGPPGGR
     GPKGEKGNEG LCACEPGPMG PPGPPGLPGR QGSKGDLGLP GWLGTKGDPG PPGAEGPPGL
     PGKHGASGPP GNKGAKGDMV VSRVKGHKGE RGPDGPPGFP GQPGSHGRDG HAGEKGDPGP
     PGDHEDATPG GKGFPGPLGP PGKAGPVGPP GLGFPGPPGE RGHPGVPGHP GVRGPDGLKG
     QKGDTISCNV TYPGRHGPPG FDGPPGPKGF PGPQGAPGLS GSDGHKGRPG TPGTAEIPGP
     PGFRGDMGDP GFGGEKGSSP VGPPGPPGSP GVNGQKGIPG DPAFGHLGPP GKRGLSGVPG
     IKGPRGDPGC PGAEGPAGIP GFLGLKGPKG REGHAGFPGV PGPPGHSCER GAPGIPGQPG
     LPGYPGSPGA PGGKGQPGDV GPPGPAGMKG LPGLPGRPGA HGPPGLPGIP GPFGDDGLPG
     PPGPKGPRGL PGFPGFPGER GKPGAEGCPG AKGEPGEKGM SGLPGDRGLR GAKGAIGPPG
     DEGEMAIISQ KGTPGEPGPP GDDGFPGERG DKGTPGMQGR RGEPGRYGPP GFHRGEPGEK
     GQPGPPGPPG PPGSTGLRGF IGFPGLPGDQ GEPGSPGPPG FSGIDGARGP KGNKGDPASH
     FGPPGPKGEP GSPGCPGHFG ASGEQGLPGI QGPRGSPGRP GPPGSSGPPG CPGDHGMPGL
     RGQPGEMGDP GPRGLQGDPG IPGPPGIKGP SGSPGLNGLH GLKGQKGTKG ASGLHDVGPP
     GPVGIPGLKG ERGDPGSPGI SPPGPRGKKG PPGPPGSSGP PGPAGATGRA PKDIPDPGPP
     GDQGPPGPDG PRGAPGPPGL PGSVDLLRGE PGDCGLPGPP GPPGPPGPPG YKGFPGCDGK
     DGQKGPVGFP GPQGPHGFPG PPGEKGLPGP PGRKGPTGLP GPRGEPGPPA DVDDCPRIPG
     LPGAPGMRGP EGAMGLPGMR GPSGPGCKGE PGLDGRRGVD GVPGSPGPPG RKGDTGEDGY
     PGGPGPPGPI GDPGPKGFGP GYLGGFLLVL HSQTDQEPTC PLGMPRLWTG YSLLYLEGQE
     KAHNQDLGLA GSCLPVFSTL PFAYCNIHQV CHYAQRNDRS YWLASAAPLP MMPLSEEAIR
     PYVSRCAVCE APAQAVAVHS QDQSIPPCPQ TWRSLWIGYS FLMHTGAGDQ GGGQALMSPG
     SCLEDFRAAP FLECQGRQGT CHFFANKYSF WLTTVKADLQ FSSAPAPDTL KESQAQRQKI
     SRCQVCVKYS
 
 
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