ID   ACLDH_PSEAI             Reviewed;         506 AA.
AC   Q9ZAA1;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Acetaldehyde dehydrogenase {ECO:0000303|PubMed:10075429};
DE            EC=1.2.1.- {ECO:0000269|PubMed:10075429};
GN   Name=exaC {ECO:0000303|PubMed:10075429, ECO:0000312|EMBL:AAC79659.1};
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, INDUCTION,
RP   AND PATHWAY.
RC   STRAIN=ATCC 17933 / 633;
RX   PubMed=10075429; DOI=10.1099/13500872-145-2-471;
RA   Schobert M., Goerisch H.;
RT   "Cytochrome c550 is an essential component of the quinoprotein ethanol
RT   oxidation system in Pseudomonas aeruginosa: cloning and sequencing of the
RT   genes encoding cytochrome c550 and an adjacent acetaldehyde
RT   dehydrogenase.";
RL   Microbiology 145:471-481(1999).
CC   -!- FUNCTION: Catalyzes the NAD(+)-dependent oxidation of acetaldehyde to
CC       acetate. Is likely a component of the ethanol oxidation system that
CC       allows P.aeruginosa to grow on ethanol as the sole carbon and energy
CC       source. {ECO:0000269|PubMed:10075429}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetaldehyde + H2O + NAD(+) = acetate + 2 H(+) + NADH;
CC         Xref=Rhea:RHEA:25294, ChEBI:CHEBI:15343, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; Evidence={ECO:0000269|PubMed:10075429};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25295;
CC         Evidence={ECO:0000305|PubMed:10075429};
CC   -!- PATHWAY: Alcohol metabolism; ethanol degradation; acetate from ethanol:
CC       step 2/2. {ECO:0000305|PubMed:10075429}.
CC   -!- INDUCTION: Induced by growth on ethanol. {ECO:0000305|PubMed:10075429}.
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF068264; AAC79659.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9ZAA1; -.
DR   SMR; Q9ZAA1; -.
DR   UniPathway; UPA00780; UER00768.
DR   GO; GO:0043878; F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (non-phosphorylating) activity; IEA:RHEA.
DR   GO; GO:0006068; P:ethanol catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR016160; Ald_DH_CS_CYS.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00070; ALDEHYDE_DEHYDR_CYS; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   NAD; Oxidoreductase.
FT   CHAIN           1..506
FT                   /note="Acetaldehyde dehydrogenase"
FT                   /id="PRO_0000449348"
FT   ACT_SITE        262
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT   ACT_SITE        301
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10008"
SQ   SEQUENCE   506 AA;  54853 MW;  674995B6EB985C42 CRC64;
     MIYAAPGTPG AVVTFKPRYG NYIGGEFVPP VKGQYFTNTS PVNGQPIAEF PRSTAEDIDK
     ALDAAHAAAD AWGRTSVQER SNILLKIADR IEQNLELLAV TETWDNGKAV RETLNADIPL
     AADHFRYFAG CIRAQEGSAA EINDSTVAYH IHEPLGVVGQ IIPWNFPLLM AAWKLAPALA
     AGNCVVLKPA EQTPLGICVL LELIGDLLPP GVLNVVQGFG REAGEALATS KRIAKIAFTG
     STPVGSHILK CAAESIIPST VELGGKSPNI YFEDIMQAEP AFIEKAAEGL VLAFFNQGEV
     CTCPSRALVQ ESIYPAFMEE VLKKVRAIKR GDPLDTETMV GAQASQQQYE KILSYLDIAQ
     QEGAELLAGG SVEKLEGNLA SGYYIQPTLL KGHNGMRVFQ EEIFGPVVGV TTFKDEAEAL
     AIANDTEYGL GAGLWTRDIN RAYRMGRGIK AGRVWTNCYH LYPAHAAFGG YKKSGVGRET
     HKMMLDHYQQ TKNLLVSYDI DPLGFF