COA3_YEAST
ID COA3_YEAST Reviewed; 85 AA.
AC Q3E7B2; D6VWB9;
DT 11-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Cytochrome c oxidase assembly factor 3, mitochondrial;
DE AltName: Full=Cytochrome c oxidase protein 25;
DE AltName: Full=Required for respiratory growth protein 10;
GN Name=COA3; Synonyms=COX25, RRG10; OrderedLocusNames=YJL062W-A;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP GENOME REANNOTATION.
RX PubMed=11152879; DOI=10.1016/s0014-5793(00)02275-4;
RA Blandin G., Durrens P., Tekaia F., Aigle M., Bolotin-Fukuhara M., Bon E.,
RA Casaregola S., de Montigny J., Gaillardin C., Lepingle A., Llorente B.,
RA Malpertuy A., Neuveglise C., Ozier-Kalogeropoulos O., Perrin A., Potier S.,
RA Souciet J.-L., Talla E., Toffano-Nioche C., Wesolowski-Louvel M., Marck C.,
RA Dujon B.;
RT "Genomic exploration of the hemiascomycetous yeasts: 4. The genome of
RT Saccharomyces cerevisiae revisited.";
RL FEBS Lett. 487:31-36(2000).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP FUNCTION.
RX PubMed=16510898; DOI=10.1101/gr.4355406;
RA Kastenmayer J.P., Ni L., Chu A., Kitchen L.E., Au W.-C., Yang H.,
RA Carter C.D., Wheeler D., Davis R.W., Boeke J.D., Snyder M.A., Basrai M.A.;
RT "Functional genomics of genes with small open reading frames (sORFs) in S.
RT cerevisiae.";
RL Genome Res. 16:365-373(2006).
RN [7]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=16823961; DOI=10.1021/pr050477f;
RA Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.;
RT "Toward the complete yeast mitochondrial proteome: multidimensional
RT separation techniques for mitochondrial proteomics.";
RL J. Proteome Res. 5:1543-1554(2006).
RN [8]
RP FUNCTION.
RX PubMed=19751518; DOI=10.1186/gb-2009-10-9-r95;
RA Merz S., Westermann B.;
RT "Genome-wide deletion mutant analysis reveals genes required for
RT respiratory growth, mitochondrial genome maintenance and mitochondrial
RT protein synthesis in Saccharomyces cerevisiae.";
RL Genome Biol. 10:R95.1-R95.18(2009).
RN [9]
RP IDENTIFICATION IN COA COMPLEXES, IDENTIFICATION BY MASS SPECTROMETRY,
RP INTERACTION WITH SHY1; COX1 AND MSS51, SUBCELLULAR LOCATION, TOPOLOGY, AND
RP FUNCTION.
RX PubMed=20876281; DOI=10.1083/jcb.201007026;
RA Mick D.U., Vukotic M., Piechura H., Meyer H.E., Warscheid B., Deckers M.,
RA Rehling P.;
RT "Coa3 and Cox14 are essential for negative feedback regulation of COX1
RT translation in mitochondria.";
RL J. Cell Biol. 191:141-154(2010).
RN [10]
RP IDENTIFICATION IN COA COMPLEXES, IDENTIFICATION BY MASS SPECTROMETRY,
RP INTERACTION WITH COX1, SUBCELLULAR LOCATION, TOPOLOGY, AND FUNCTION.
RX PubMed=21068384; DOI=10.1074/jbc.m110.188805;
RA Fontanesi F., Clemente P., Barrientos A.;
RT "Cox25 teams up with Mss51, Ssc1, and Cox14 to regulate mitochondrial
RT cytochrome c oxidase subunit 1 expression and assembly in Saccharomyces
RT cerevisiae.";
RL J. Biol. Chem. 286:555-566(2011).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for assembly of cytochrome c oxidase (complex IV).
CC With COX14, regulates negatively COX1 translation and is involved in
CC MSS51 association with newly synthesized COX1.
CC {ECO:0000269|PubMed:16510898, ECO:0000269|PubMed:19751518,
CC ECO:0000269|PubMed:20876281, ECO:0000269|PubMed:21068384}.
CC -!- SUBUNIT: Component of 250-400 kDa complexes called cytochrome oxidase
CC assembly intermediates or COA complexes composed at least COA3, COX14,
CC COX5A, SHY1 and SSC1. Interacts with COX1 and MSS51.
CC {ECO:0000269|PubMed:20876281, ECO:0000269|PubMed:21068384}.
CC -!- INTERACTION:
CC Q3E7B2; P32335: MSS51; NbExp=4; IntAct=EBI-3680840, EBI-11318;
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:16823961,
CC ECO:0000269|PubMed:20876281, ECO:0000269|PubMed:21068384}; Single-pass
CC membrane protein {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:16823961, ECO:0000269|PubMed:20876281,
CC ECO:0000269|PubMed:21068384}.
CC -!- MISCELLANEOUS: Present with 2530 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the COA3 family. {ECO:0000305}.
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DR EMBL; Z49338; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BK006943; DAA08735.1; -; Genomic_DNA.
DR RefSeq; NP_076894.3; NM_001184477.3.
DR AlphaFoldDB; Q3E7B2; -.
DR BioGRID; 33691; 205.
DR ComplexPortal; CPX-1423; COX1 pre-assembly complex.
DR IntAct; Q3E7B2; 5.
DR MINT; Q3E7B2; -.
DR STRING; 4932.YJL062W-A; -.
DR MaxQB; Q3E7B2; -.
DR PaxDb; Q3E7B2; -.
DR PRIDE; Q3E7B2; -.
DR EnsemblFungi; YJL062W-A_mRNA; YJL062W-A; YJL062W-A.
DR GeneID; 853383; -.
DR KEGG; sce:YJL062W-A; -.
DR SGD; S000007611; COA3.
DR VEuPathDB; FungiDB:YJL062W-A; -.
DR eggNOG; ENOG502S440; Eukaryota.
DR HOGENOM; CLU_153999_0_0_1; -.
DR InParanoid; Q3E7B2; -.
DR OMA; WKMTPAM; -.
DR BioCyc; YEAST:G3O-31802-MON; -.
DR PRO; PR:Q3E7B2; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; Q3E7B2; protein.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; IMP:SGD.
DR GO; GO:0070130; P:negative regulation of mitochondrial translation; IMP:SGD.
DR GO; GO:0050821; P:protein stabilization; IC:ComplexPortal.
DR InterPro; IPR041752; Coa3.
DR PANTHER; PTHR15642; PTHR15642; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..85
FT /note="Cytochrome c oxidase assembly factor 3,
FT mitochondrial"
FT /id="PRO_0000245412"
FT TOPO_DOM 1..26
FT /note="Mitochondrial matrix"
FT TRANSMEM 27..49
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 50..85
FT /note="Mitochondrial intermembrane"
SQ SEQUENCE 85 AA; 9882 MW; 41403CD21F898C18 CRC64;
MVLNPSKYQD TRTWKMTPAM IRARKPFFKG NMLGLTLLLG VTGSVYYYTY HFLHKDNDFA
DVPIPPIDPQ ELEALKKEYE AKKKA
