2AB2E_ARATH
ID 2AB2E_ARATH Reviewed; 529 AA.
AC Q84JI6; F4I2K5; Q9ZWB6;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Probable serine/threonine protein phosphatase 2A regulatory subunit B''epsilon;
DE Short=AtB''epsilon;
GN Name=B''EPSILON; OrderedLocusNames=At1g03960; ORFNames=F21M11.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=21478440; DOI=10.1105/tpc.110.074278;
RA Leivar P., Antolin-Llovera M., Ferrero S., Closa M., Arro M., Ferrer A.,
RA Boronat A., Campos N.;
RT "Multilevel control of Arabidopsis 3-hydroxy-3-methylglutaryl coenzyme A
RT reductase by protein phosphatase 2A.";
RL Plant Cell 23:1494-1511(2011).
CC -!- FUNCTION: Probable regulatory subunit of type 2A protein phosphatase.
CC {ECO:0000250}.
CC -!- SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed
CC of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant
CC regulatory subunit (PR65 or subunit A), that associates with a variety
CC of regulatory subunits. Proteins that associate with the core dimer
CC include three families of regulatory subunits B (the R2/B/PR55/B55,
CC R3/B''/PR72/PR130/PR59 and R5/B'/B56 families) and cell signaling
CC molecules (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q84JI6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q84JI6-2; Sequence=VSP_046804;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD10674.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC003027; AAD10674.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27638.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE27639.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM57805.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM57808.1; -; Genomic_DNA.
DR EMBL; BT004045; AAO42077.1; -; mRNA.
DR EMBL; BT004945; AAO50478.1; -; mRNA.
DR PIR; E86170; E86170.
DR RefSeq; NP_001320288.1; NM_001331448.1. [Q84JI6-2]
DR RefSeq; NP_001320291.1; NM_001331449.1. [Q84JI6-2]
DR RefSeq; NP_171892.2; NM_100277.5. [Q84JI6-1]
DR RefSeq; NP_973757.1; NM_202028.3. [Q84JI6-2]
DR AlphaFoldDB; Q84JI6; -.
DR SMR; Q84JI6; -.
DR BioGRID; 24596; 5.
DR STRING; 3702.AT1G03960.1; -.
DR iPTMnet; Q84JI6; -.
DR PaxDb; Q84JI6; -.
DR PRIDE; Q84JI6; -.
DR EnsemblPlants; AT1G03960.1; AT1G03960.1; AT1G03960. [Q84JI6-1]
DR EnsemblPlants; AT1G03960.2; AT1G03960.2; AT1G03960. [Q84JI6-2]
DR EnsemblPlants; AT1G03960.3; AT1G03960.3; AT1G03960. [Q84JI6-2]
DR EnsemblPlants; AT1G03960.4; AT1G03960.4; AT1G03960. [Q84JI6-2]
DR GeneID; 839361; -.
DR Gramene; AT1G03960.1; AT1G03960.1; AT1G03960. [Q84JI6-1]
DR Gramene; AT1G03960.2; AT1G03960.2; AT1G03960. [Q84JI6-2]
DR Gramene; AT1G03960.3; AT1G03960.3; AT1G03960. [Q84JI6-2]
DR Gramene; AT1G03960.4; AT1G03960.4; AT1G03960. [Q84JI6-2]
DR KEGG; ath:AT1G03960; -.
DR Araport; AT1G03960; -.
DR TAIR; locus:2024254; AT1G03960.
DR eggNOG; KOG2562; Eukaryota.
DR HOGENOM; CLU_019589_3_0_1; -.
DR InParanoid; Q84JI6; -.
DR OMA; ITANEMQ; -.
DR PhylomeDB; Q84JI6; -.
DR PRO; PR:Q84JI6; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84JI6; baseline and differential.
DR Genevisible; Q84JI6; AT.
DR GO; GO:0000159; C:protein phosphatase type 2A complex; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central.
DR GO; GO:0006470; P:protein dephosphorylation; IBA:GO_Central.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR041534; EF-hand_13.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF17958; EF-hand_13; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR SUPFAM; SSF47473; SSF47473; 2.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Calcium; Metal-binding; Reference proteome.
FT CHAIN 1..529
FT /note="Probable serine/threonine protein phosphatase 2A
FT regulatory subunit B''epsilon"
FT /id="PRO_0000422791"
FT DOMAIN 381..416
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 58..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 507..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 394
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 396
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 398
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 405
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VAR_SEQ 1..140
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046804"
SQ SEQUENCE 529 AA; 61239 MW; AA71B4C3720ED05A CRC64;
MDIDGVDDDL HILDPELLQL PGLSPSPLKP TSLIADDLFS QWLSLPETAT LVKSLIDDAK
SGTPTNKSKN LPSVFLSSST PPLSPRSSSG SPRFSRQRTS PPSLHSPLRS LKEPKRQLIP
QFYYQHGRPP AKELKEQCLS MVDQVFSNYI DGLHVDEFKS ITKQVCKLPS FLSPALFRKI
DPNCTDIVTR DAFIKYWIDG NMLTMDTASQ IYNILRQQGC SYLRQADFKP VLDELLATHP
GLEFLRTISE FQERYAETVI YRIFYYINRS GTGCLTLREL RRGNLIAAMQ QLDEEDDINK
IIRYFSYEHF YVIYCKFWEL DGDHDCFIDK DNLIKYGNNA LTYRIVDRIF SQIPRKFTSK
VEGKMSYEDF VYFILAEEDK SSEPSLEYWF KCVDLDGNGV ITSNEMQFFF EEQLHRMECI
TQEAVLFSDI LCQIIDMIGP EKENCITLQD LKGSKLSANV FNILFNLNKF MAFETRDPFL
IRQEREDPNL TEWDRFAQRE YARLSMEEDV DEVSNGSADV WDEPLEPPF
