ID   COA8_BOVIN              Reviewed;         192 AA.
AC   Q148E1;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   03-AUG-2022, entry version 82.
DE   RecName: Full=Cytochrome c oxidase assembly factor 8 {ECO:0000305};
DE            Short=COA8;
DE   AltName: Full=Apoptogenic protein 1, mitochondrial;
DE            Short=APOP-1;
DE   Flags: Precursor;
GN   Name=COA8; Synonyms=APOP1, APOPT1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal cerebellum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Required for cytochrome c complex (COX) IV assembly and
CC       function Protects COX assembly from oxidation-induced degradation, COX
CC       being the terminal component of the mitochondrial respiratory chain.
CC       {ECO:0000250|UniProtKB:Q96IL0}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q9CQW7}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q96IL0}; Matrix side
CC       {ECO:0000250|UniProtKB:Q96IL0}.
CC   -!- INDUCTION: In conditions of increased oxidative stress, the protein is
CC       stabilized, increasing its mature intramitochondrial form and thereby
CC       protecting COX from oxidatively induced degradation.
CC       {ECO:0000250|UniProtKB:Q96IL0, ECO:0000250|UniProtKB:Q9CQW7}.
CC   -!- PTM: N-terminal mitochondrial targeting sequence is cleaved from the
CC       mature protein once in the mitochondrion.
CC       {ECO:0000250|UniProtKB:Q96IL0}.
CC   -!- PTM: In normal conditions, the cytoplasmic precursor protein is rapidly
CC       degraded by the ubiquitination-proteasome system (UPS). Oxidative
CC       stress induces protein stabilization and import into mitochondria where
CC       it protects COX from degradation. {ECO:0000250|UniProtKB:Q96IL0}.
CC   -!- SIMILARITY: Belongs to the COA8 family. {ECO:0000305}.
CC   -!- CAUTION: First thought to play a role in the regulation of apoptosis,
CC       mediating mitochondria-induced cell death in vascular smooth muscle
CC       cells through the release of cytochrome c (COX) from mitochondria and
CC       the activation of the caspase cascade. However, recent studies show
CC       that it is not directly involved in apoptosis regulation but in the
CC       protection of COX from oxidatively induced degradation.
CC       {ECO:0000250|UniProtKB:Q96IL0, ECO:0000250|UniProtKB:Q9CQW7}.
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DR   EMBL; BC118424; AAI18425.1; -; mRNA.
DR   RefSeq; NP_001069927.1; NM_001076459.2.
DR   AlphaFoldDB; Q148E1; -.
DR   STRING; 9913.ENSBTAP00000028246; -.
DR   PaxDb; Q148E1; -.
DR   Ensembl; ENSBTAT00000028246; ENSBTAP00000028246; ENSBTAG00000021199.
DR   GeneID; 617441; -.
DR   KEGG; bta:617441; -.
DR   CTD; 84334; -.
DR   VEuPathDB; HostDB:ENSBTAG00000021199; -.
DR   VGNC; VGNC:26037; COA8.
DR   eggNOG; KOG4094; Eukaryota.
DR   GeneTree; ENSGT00390000008212; -.
DR   HOGENOM; CLU_118274_0_0_1; -.
DR   InParanoid; Q148E1; -.
DR   OMA; TRHNKRF; -.
DR   OrthoDB; 1410150at2759; -.
DR   TreeFam; TF315168; -.
DR   Proteomes; UP000009136; Chromosome 21.
DR   Bgee; ENSBTAG00000021199; Expressed in semen and 105 other tissues.
DR   GO; GO:0099617; C:matrix side of mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0097193; P:intrinsic apoptotic signaling pathway; IEA:Ensembl.
DR   GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; ISS:UniProtKB.
DR   GO; GO:1903427; P:negative regulation of reactive oxygen species biosynthetic process; ISS:UniProtKB.
DR   GO; GO:1904960; P:positive regulation of cytochrome-c oxidase activity; ISS:UniProtKB.
DR   GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR   GO; GO:0000302; P:response to reactive oxygen species; ISS:UniProtKB.
DR   InterPro; IPR018796; COA8.
DR   PANTHER; PTHR31107; PTHR31107; 1.
DR   Pfam; PF10231; DUF2315; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Ubl conjugation.
FT   TRANSIT         1..10
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           11..192
FT                   /note="Cytochrome c oxidase assembly factor 8"
FT                   /id="PRO_0000353107"
SQ   SEQUENCE   192 AA;  22911 MW;  79AC207C5641BD72 CRC64;
     MAPRRPAKRL VTALCRAFSG RGCQLAPKRG AERRDAAPSR VSRFCPPRKS RHDWIGPPDK
     YSNLRPIHFY IPENESPLEQ KLRELRQETQ EWNQQFWADQ NLTFHKEKEE FVRSRLKAKG
     LDLRTASGQK ATLNAEEMAE FYKEFLSKNF QKHMYYNRDW YKRNFAITFF MGKVALERIW
     NKLRPKQKKT SS