ID   ACLN_ASPOR              Reviewed;         238 AA.
AC   Q2UPB6;
DT   30-AUG-2017, integrated into UniProtKB/Swiss-Prot.
DT   24-JAN-2006, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=Aspirochlorine biosynthesis protein N {ECO:0000303|PubMed:25302411};
GN   Name=aclN {ECO:0000303|PubMed:25302411}; ORFNames=AO090001000036;
OS   Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=510516;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 42149 / RIB 40;
RX   PubMed=16372010; DOI=10.1038/nature04300;
RA   Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA   Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA   Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA   Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA   Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA   Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA   Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA   Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA   Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA   Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA   Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA   Kikuchi H.;
RT   "Genome sequencing and analysis of Aspergillus oryzae.";
RL   Nature 438:1157-1161(2005).
RN   [2]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=25302411; DOI=10.1002/anie.201407624;
RA   Chankhamjon P., Boettger-Schmidt D., Scherlach K., Urbansky B., Lackner G.,
RA   Kalb D., Dahse H.M., Hoffmeister D., Hertweck C.;
RT   "Biosynthesis of the halogenated mycotoxin aspirochlorine in koji mold
RT   involves a cryptic amino acid conversion.";
RL   Angew. Chem. Int. Ed. 53:13409-13413(2014).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       aspirochlorine (or antibiotic A30641), an unusual halogenated spiro
CC       compound with distinctive antifungal properties due to selective
CC       inhibition of protein biosynthesis, and which is also active against
CC       bacteria, viruses, and murine tumor cells (PubMed:25302411). The non-
CC       ribosomal peptide synthetase (NRPS) aclP is responsible the formation
CC       of the diketopiperazine (DKP) core from the condensation of 2
CC       phenylalanine residues (PubMed:25302411). One Phe residue is tailored
CC       into chlorotyrosine by hydroxylation and chlorination, whereas the
CC       second Phe undergoes an unprecedented C-C bond cleavage to be converted
CC       into glycine (PubMed:25302411). After formation of the DKP, sulfur is
CC       incorporated into the DKP by conjugation with glutathione by aclG,
CC       followed by its stepwise degradation to the thiol by aclI, aclJ and
CC       aclK, and the dithiol oxidation by aclT (PubMed:25302411). In addition,
CC       oxygenases (aclB, aclC, aclL and aclO) and O-methyltransferases (aclM
CC       and aclU) act as tailoring enzymes to produce the intermediate
CC       dechloroaspirochlorine (PubMed:25302411). Ultimately, chlorination of
CC       dechloroaspirochlorine by the halogenase aclH is the last step in the
CC       aspirochlorine pathway (PubMed:25302411).
CC       {ECO:0000269|PubMed:25302411}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:25302411}.
CC   -!- SIMILARITY: Belongs to the asaB hydroxylase/desaturase family.
CC       {ECO:0000305}.
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DR   EMBL; AP007154; BAE56599.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q2UPB6; -.
DR   SMR; Q2UPB6; -.
DR   EnsemblFungi; BAE56599; BAE56599; AO090001000036.
DR   HOGENOM; CLU_042688_0_1_1; -.
DR   Proteomes; UP000006564; Chromosome 2.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR   InterPro; IPR044053; AsaB-like.
DR   PANTHER; PTHR34598; PTHR34598; 1.
PE   3: Inferred from homology;
KW   Reference proteome.
FT   CHAIN           1..238
FT                   /note="Aspirochlorine biosynthesis protein N"
FT                   /id="PRO_0000441194"
SQ   SEQUENCE   238 AA;  26992 MW;  D3487959B2948B3A CRC64;
     MSRSTMLAHS AIPSSLIRMD LLSPWILTAT APKFLRLFAP ATRRRSARST IPSWSRSSSG
     KQAHHAWLSS ITPCDGARSP WLARIPMAAS SLQAQSPLGA RRRVYQHLPE EADRLLKGRA
     QIVNVWRPLR GPVQDWPLAV MDCSTLAQAH IHPTKLYRNR FELRGETVSI SHDESQRWYY
     LDGQQTDECT LIKIWDSKEG ISGHMCAHCA FQHPNTPVDA PLRESVEVRC LVFYENQE