ID   COA8_RAT                Reviewed;         193 AA.
AC   Q32Q90;
DT   04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Cytochrome c oxidase assembly factor 8;
DE            Short=COA8;
DE   AltName: Full=Apoptogenic protein 1, mitochondrial;
DE            Short=APOP-1;
DE   Flags: Precursor;
GN   Name=Coa8; Synonyms=Apop1, Apopt1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Required for cytochrome c complex (COX) IV assembly and
CC       function Protects COX assembly from oxidation-induced degradation, COX
CC       being the terminal component of the mitochondrial respiratory chain.
CC       {ECO:0000250|UniProtKB:Q96IL0, ECO:0000250|UniProtKB:Q9CQW7}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q9CQW7}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q96IL0}; Matrix side
CC       {ECO:0000250|UniProtKB:Q96IL0}.
CC   -!- INDUCTION: In conditions of increased oxidative stress, the protein is
CC       stabilized, increasing its mature intramitochondrial form and thereby
CC       protecting COX from oxidatively induced degradation.
CC       {ECO:0000250|UniProtKB:Q96IL0, ECO:0000250|UniProtKB:Q9CQW7}.
CC   -!- PTM: N-terminal mitochondrial targeting sequence is cleaved from the
CC       mature protein once in the mitochondrion.
CC       {ECO:0000250|UniProtKB:Q96IL0}.
CC   -!- PTM: In normal conditions, the cytoplasmic precursor protein is rapidly
CC       degraded by the ubiquitination-proteasome system (UPS). Oxidative
CC       stress induces protein stabilization and import into mitochondria where
CC       it protects COX from degradation. {ECO:0000250|UniProtKB:Q96IL0}.
CC   -!- SIMILARITY: Belongs to the COA8 family. {ECO:0000305}.
CC   -!- CAUTION: First thought to play a role in the regulation of apoptosis,
CC       mediating mitochondria-induced cell death in vascular smooth muscle
CC       cells through the release of cytochrome c (COX) from mitochondria and
CC       the activation of the caspase cascade. However, recent studies show
CC       that it is not directly involved in apoptosis regulation but in the
CC       protection of COX from oxidatively induced degradation.
CC       {ECO:0000250|UniProtKB:Q96IL0, ECO:0000250|UniProtKB:Q9CQW7}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC107661; AAI07662.1; -; mRNA.
DR   RefSeq; NP_001032858.1; NM_001037769.2.
DR   RefSeq; XP_017450002.1; XM_017594513.1.
DR   RefSeq; XP_017458789.1; XM_017603300.1.
DR   AlphaFoldDB; Q32Q90; -.
DR   SMR; Q32Q90; -.
DR   STRING; 10116.ENSRNOP00000015373; -.
DR   PaxDb; Q32Q90; -.
DR   PRIDE; Q32Q90; -.
DR   GeneID; 299341; -.
DR   KEGG; rno:299341; -.
DR   UCSC; RGD:1304719; rat.
DR   CTD; 84334; -.
DR   RGD; 1304719; Apopt1.
DR   VEuPathDB; HostDB:ENSRNOG00000011542; -.
DR   eggNOG; KOG4094; Eukaryota.
DR   HOGENOM; CLU_118274_0_0_1; -.
DR   InParanoid; Q32Q90; -.
DR   OMA; TRHNKRF; -.
DR   OrthoDB; 1410150at2759; -.
DR   PhylomeDB; Q32Q90; -.
DR   TreeFam; TF315168; -.
DR   PRO; PR:Q32Q90; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000011542; Expressed in pancreas and 19 other tissues.
DR   Genevisible; Q32Q90; RN.
DR   GO; GO:0099617; C:matrix side of mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0097193; P:intrinsic apoptotic signaling pathway; ISO:RGD.
DR   GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; ISS:UniProtKB.
DR   GO; GO:1903427; P:negative regulation of reactive oxygen species biosynthetic process; ISS:UniProtKB.
DR   GO; GO:1904960; P:positive regulation of cytochrome-c oxidase activity; ISS:UniProtKB.
DR   GO; GO:0050821; P:protein stabilization; ISS:UniProtKB.
DR   GO; GO:0000302; P:response to reactive oxygen species; ISS:UniProtKB.
DR   InterPro; IPR018796; COA8.
DR   PANTHER; PTHR31107; PTHR31107; 1.
DR   Pfam; PF10231; DUF2315; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Transit peptide; Ubl conjugation.
FT   TRANSIT         1..26
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..193
FT                   /note="Cytochrome c oxidase assembly factor 8"
FT                   /id="PRO_0000353109"
SQ   SEQUENCE   193 AA;  22769 MW;  36AB715828B7EDFF CRC64;
     MAALRPGSKA LRRLLCRSFS GGGVRLARER ATERRDAASS RVSRFCPPRQ SCHDWIGPPD
     KYSNLRPVHF HVPENESPLE QRLRELRQET QEWNQQFWAK QNLSFNKEKE EFIYSRLQAK
     GSGPRTESGQ RATLDAEEMA DFYKDFLSKN FQKHMCYNRD WYKRNFAITF FMGKVALERM
     WSKLKQKPKK TSG