ACM1_CAEEL
ID ACM1_CAEEL Reviewed; 713 AA.
AC Q18007; Q9NJS7; Q9NJS8; Q9XTK1;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 3.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Probable muscarinic acetylcholine receptor gar-1;
DE AltName: Full=G-protein-linked acetylcholine receptor 1;
GN Name=gar-1; ORFNames=C15B12.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RC STRAIN=Bristol N2;
RX PubMed=9886054; DOI=10.1046/j.1471-4159.1999.0720058.x;
RA Lee Y.-S., Park Y.-S., Chang D.-J., Hwang J.M., Min C.K., Kaang B.-K.,
RA Cho N.J.;
RT "Cloning and expression of a G protein-linked acetylcholine receptor from
RT Caenorhabditis elegans.";
RL J. Neurochem. 72:58-65(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND ALTERNATIVE SPLICING (ISOFORMS B AND C).
RC STRAIN=Bristol N2;
RX PubMed=10679207; DOI=10.1006/bbrc.2000.2108;
RA Park Y.-S., Lee Y.-S., Cho N.J., Kaang B.-K.;
RT "Alternative splicing of gar-1, a Caenorhabditis elegans G-protein-linked
RT acetylcholine receptor gene.";
RL Biochem. Biophys. Res. Commun. 268:354-358(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=11032868; DOI=10.1046/j.1471-4159.2000.0751800.x;
RA Lee Y.-S., Park Y.-S., Nam S., Suh S.J., Lee J., Kaang B.-K., Cho N.J.;
RT "Characterization of GAR-2, a novel G protein-linked acetylcholine receptor
RT from Caenorhabditis elegans.";
RL J. Neurochem. 75:1800-1809(2000).
CC -!- FUNCTION: The muscarinic acetylcholine receptor mediates various
CC cellular responses, including inhibition of adenylate cyclase,
CC breakdown of phosphoinositides and modulation of potassium channels
CC through the action of G proteins. Primary transducing effect is Pi
CC turnover.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=B; Synonyms=A, Large;
CC IsoId=Q18007-1; Sequence=Displayed;
CC Name=A; Synonyms=B, Medium;
CC IsoId=Q18007-2; Sequence=VSP_001860;
CC Name=C; Synonyms=Small;
CC IsoId=Q18007-3; Sequence=VSP_001860, VSP_001861;
CC -!- TISSUE SPECIFICITY: Expressed in head region of the larva. In adults,
CC expression is seen in the periventricularis magnocellularis (PVM)
CC neuron. {ECO:0000269|PubMed:11032868}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development from embryonic to
CC adult stage, highest level seen in early larvae. Isoform A is the most
CC abundant and isoform C is the least.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Muscarinic acetylcholine receptor subfamily. {ECO:0000255|PROSITE-
CC ProRule:PRU00521}.
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DR EMBL; AF075245; AAD13747.1; -; mRNA.
DR EMBL; AF117300; AAF26201.1; -; mRNA.
DR EMBL; AF117301; AAF26202.1; -; mRNA.
DR EMBL; FO080547; CCD64566.1; -; Genomic_DNA.
DR EMBL; FO080547; CCD64567.1; -; Genomic_DNA.
DR EMBL; FO080547; CCD64568.1; -; Genomic_DNA.
DR PIR; T43292; T43292.
DR RefSeq; NP_001024401.1; NM_001029230.1. [Q18007-2]
DR RefSeq; NP_001024402.1; NM_001029231.1. [Q18007-1]
DR RefSeq; NP_001024403.1; NM_001029232.1. [Q18007-3]
DR AlphaFoldDB; Q18007; -.
DR SMR; Q18007; -.
DR STRING; 6239.C15B12.5b; -.
DR PaxDb; Q18007; -.
DR EnsemblMetazoa; C15B12.5a.1; C15B12.5a.1; WBGene00001517. [Q18007-2]
DR EnsemblMetazoa; C15B12.5b.1; C15B12.5b.1; WBGene00001517. [Q18007-1]
DR EnsemblMetazoa; C15B12.5c.1; C15B12.5c.1; WBGene00001517. [Q18007-3]
DR GeneID; 180922; -.
DR KEGG; cel:CELE_C15B12.5; -.
DR UCSC; C15B12.5c; c. elegans. [Q18007-1]
DR CTD; 180922; -.
DR WormBase; C15B12.5a; CE29666; WBGene00001517; gar-1. [Q18007-2]
DR WormBase; C15B12.5b; CE29667; WBGene00001517; gar-1. [Q18007-1]
DR WormBase; C15B12.5c; CE29668; WBGene00001517; gar-1. [Q18007-3]
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT00940000172795; -.
DR InParanoid; Q18007; -.
DR OMA; PYVNMGM; -.
DR OrthoDB; 1245472at2759; -.
DR PhylomeDB; Q18007; -.
DR PRO; PR:Q18007; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00001517; Expressed in larva and 3 other tissues.
DR ExpressionAtlas; Q18007; baseline and differential.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IDA:WormBase.
DR GO; GO:0016907; F:G protein-coupled acetylcholine receptor activity; IDA:WormBase.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0007213; P:G protein-coupled acetylcholine receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0007271; P:synaptic transmission, cholinergic; IDA:WormBase.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000995; Musac_Ach_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00243; MUSCARINICR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..713
FT /note="Probable muscarinic acetylcholine receptor gar-1"
FT /id="PRO_0000069048"
FT TOPO_DOM 1..20
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 21..41
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 42..66
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 67..87
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 88..101
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 102..122
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 123..144
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 145..165
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 166..189
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 190..210
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 211..633
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 634..656
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 657..670
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 671..693
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 694..713
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 256..350
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 381..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 427..475
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 515..585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 290..315
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 553..567
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 99..180
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 275..305
FT /note="Missing (in isoform A and isoform C)"
FT /evidence="ECO:0000303|PubMed:9886054"
FT /id="VSP_001860"
FT VAR_SEQ 533..593
FT /note="DDSSSEVEGEEKPEVRNNGLKIPQLTVNNENRGETSSQPGRDRLAPPNKTDT
FT FLSASGVSR -> E (in isoform C)"
FT /evidence="ECO:0000305"
FT /id="VSP_001861"
SQ SEQUENCE 713 AA; 80404 MW; 114EA5065876DEBF CRC64;
MPNYTVPPDP ADTSWDSPYS IPVQIVVWII IIVLSLETII GNAMVVMAYR IERNISKQVS
NRYIVSLAIS DLIIGIEGFP FFTVYVLNGD RWPLGWVACQ TWLFLDYTLC LVSILTVLLI
TADRYLSVCH TAKYLKWQSP TKTQLLIVMS WLLPAIIFGI MIYGWQAMTG QSTSMSGAEC
SAPFLSNPYV NMGMYVAYYW TTLVAMLILY KGIHQAAKNL EKKAKAKERR HIALILSQRL
GTQVGVSLML QSKAEKEKAE EAQKDSGYTS NQAGDANNLR RFGFSEPETS QFRVDPNSNN
NLNVEGSLNT ENDQNLGVIE EERSGFLSRR ESNESYYPGP HPTAANSRRC SEMEKVSLLS
ESDGVPSTRP AKSYGRLSLR SRYSASESIT TTHENDEKEV EKADSLQKLF ADDELGSVLN
FKEEKLKNTD SNNDSDTTSV ILQRSRKYKK NKRPRSSRRS EHSTPRQIAK VKQAEGTAAQ
LIEESVPDDD QTETIEVKRT DRWVVSMKKR IARALIRRRS TTRPERGSSS NSDDSSSEVE
GEEKPEVRNN GLKIPQLTVN NENRGETSSQ PGRDRLAPPN KTDTFLSASG VSRKISTIST
VITREKVISS IFAPIAVFNR GRKQTKAEKR AHKAFRTITF IVGFFAILWS PYYIMATVYG
FCKGECIPSF LYTLSYYMCY LNSSGNPFAY ALANRQFRSA FMRMFRGNFN KVA
