ACM1_HUMAN
ID ACM1_HUMAN Reviewed; 460 AA.
AC P11229; Q96RH1;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 2.
DT 03-AUG-2022, entry version 215.
DE RecName: Full=Muscarinic acetylcholine receptor M1;
GN Name=CHRM1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3697105; DOI=10.1093/nar/15.24.10604;
RA Allard W.J., Sigal I.S., Dixon R.A.F.;
RT "Sequence of the gene encoding the human M1 muscarinic acetylcholine
RT receptor.";
RL Nucleic Acids Res. 15:10604-10604(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2336407; DOI=10.1093/nar/18.8.2191;
RA Chapman C.G., Browne M.J.;
RT "Isolation of the human ml (Hml) muscarinic acetylcholine receptor gene by
RT PCR amplification.";
RL Nucleic Acids Res. 18:2191-2191(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3443095; DOI=10.1002/j.1460-2075.1987.tb02733.x;
RA Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J.,
RA Capon D.J.;
RT "Distinct primary structures, ligand-binding properties and tissue-specific
RT expression of four human muscarinic acetylcholine receptors.";
RL EMBO J. 6:3923-3929(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RA Arredondo J., Grando S.A.;
RT "Cloning Cholinergic Receptors in Human Keratinocytes.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16554811; DOI=10.1038/nature04632;
RA Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K.,
RA Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T.,
RA Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G.,
RA Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C.,
RA Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A.,
RA Hattori M., Rogers J., Lander E.S., Sakaki Y.;
RT "Human chromosome 11 DNA sequence and analysis including novel gene
RT identification.";
RL Nature 440:497-500(2006).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP MUTAGENESIS.
RX PubMed=1445347; DOI=10.1016/0006-291x(92)91346-r;
RA Arden J.R., Nagata O., Shockley M.S., Philip M., Lameh J., Sadee W.;
RT "Mutational analysis of third cytoplasmic loop domains in G-protein
RT coupling of the HM1 muscarinic receptor.";
RL Biochem. Biophys. Res. Commun. 188:1111-1115(1992).
RN [9]
RP INTERACTION WITH GPRASP2.
RX PubMed=15086532; DOI=10.1111/j.1471-4159.2004.02411.x;
RA Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.;
RT "Identification of a novel family of G protein-coupled receptor associated
RT sorting proteins.";
RL J. Neurochem. 89:766-775(2004).
RN [10]
RP INTERACTION WITH TMEM147.
RX PubMed=21056967; DOI=10.1124/mol.110.067363;
RA Rosemond E., Rossi M., McMillin S.M., Scarselli M., Donaldson J.G.,
RA Wess J.;
RT "Regulation of M(3) muscarinic receptor expression and function by
RT transmembrane protein 147.";
RL Mol. Pharmacol. 79:251-261(2011).
RN [11] {ECO:0000312|PDB:6WJC}
RP X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF 2-218 AND 355-460 IN COMPLEX WITH
RP SNAKE VENOM MUSCARINIC TOXIN 7, TOPOLOGY, AND DISULFIDE BONDS.
RX PubMed=32646996; DOI=10.1126/science.aax2517;
RA Maeda S., Xu J., Kadji F.M.N., Clark M.J., Zhao J., Tsutsumi N., Aoki J.,
RA Sunahara R.K., Inoue A., Garcia K.C., Kobilka B.K.;
RT "Structure and selectivity engineering of the M1 muscarinic receptor toxin
RT complex.";
RL Science 369:161-167(2020).
CC -!- FUNCTION: The muscarinic acetylcholine receptor mediates various
CC cellular responses, including inhibition of adenylate cyclase,
CC breakdown of phosphoinositides and modulation of potassium channels
CC through the action of G proteins. Primary transducing effect is Pi
CC turnover.
CC -!- SUBUNIT: Interacts with GPRASP2 (PubMed:15086532). Interacts with
CC TMEM147 (PubMed:21056967). {ECO:0000269|PubMed:15086532,
CC ECO:0000269|PubMed:21056967}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Postsynaptic cell membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P11229-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P11229-2; Sequence=VSP_056651;
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Muscarinic acetylcholine receptor subfamily. CHRM1 sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; Y00508; CAA68560.1; -; Genomic_DNA.
DR EMBL; X52068; CAA36291.1; -; Genomic_DNA.
DR EMBL; X15263; CAA33334.1; -; Genomic_DNA.
DR EMBL; AF385587; AAK68112.1; -; mRNA.
DR EMBL; AF498915; AAM18938.1; -; mRNA.
DR EMBL; AP000438; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC007740; AAH07740.1; -; mRNA.
DR EMBL; BC022984; AAH22984.1; -; mRNA.
DR CCDS; CCDS8040.1; -. [P11229-1]
DR PIR; S09508; S09508.
DR RefSeq; NP_000729.2; NM_000738.2. [P11229-1]
DR RefSeq; XP_011543044.1; XM_011544742.2. [P11229-1]
DR PDB; 5CXV; X-ray; 2.70 A; A=2-218, A=355-460.
DR PDB; 6OIJ; EM; 3.30 A; R=2-460.
DR PDB; 6WJC; X-ray; 2.55 A; A=2-218, A=355-460.
DR PDB; 6ZFZ; X-ray; 2.17 A; A=27-219, A=355-438.
DR PDB; 6ZG4; X-ray; 2.33 A; A=27-219, A=355-438.
DR PDB; 6ZG9; X-ray; 2.50 A; A=27-219, A=355-438.
DR PDBsum; 5CXV; -.
DR PDBsum; 6OIJ; -.
DR PDBsum; 6WJC; -.
DR PDBsum; 6ZFZ; -.
DR PDBsum; 6ZG4; -.
DR PDBsum; 6ZG9; -.
DR AlphaFoldDB; P11229; -.
DR SMR; P11229; -.
DR BioGRID; 107550; 4.
DR IntAct; P11229; 5.
DR MINT; P11229; -.
DR STRING; 9606.ENSP00000306490; -.
DR BindingDB; P11229; -.
DR ChEMBL; CHEMBL216; -.
DR DrugBank; DB03128; Acetylcholine.
DR DrugBank; DB08897; Aclidinium.
DR DrugBank; DB05766; ACP-104.
DR DrugBank; DB05752; ALKS 27.
DR DrugBank; DB00321; Amitriptyline.
DR DrugBank; DB00543; Amoxapine.
DR DrugBank; DB00517; Anisotropine methylbromide.
DR DrugBank; DB04365; Arecoline.
DR DrugBank; DB01238; Aripiprazole.
DR DrugBank; DB14185; Aripiprazole lauroxil.
DR DrugBank; DB00572; Atropine.
DR DrugBank; DB00245; Benzatropine.
DR DrugBank; DB00767; Benzquinamide.
DR DrugBank; DB01019; Bethanechol.
DR DrugBank; DB00810; Biperiden.
DR DrugBank; DB00835; Brompheniramine.
DR DrugBank; DB00354; Buclizine.
DR DrugBank; DB00411; Carbamoylcholine.
DR DrugBank; DB00185; Cevimeline.
DR DrugBank; DB00477; Chlorpromazine.
DR DrugBank; DB01239; Chlorprothixene.
DR DrugBank; DB00568; Cinnarizine.
DR DrugBank; DB00771; Clidinium.
DR DrugBank; DB00363; Clozapine.
DR DrugBank; DB00907; Cocaine.
DR DrugBank; DB00979; Cyclopentolate.
DR DrugBank; DB00942; Cycrimine.
DR DrugBank; DB00434; Cyproheptadine.
DR DrugBank; DB00496; Darifenacin.
DR DrugBank; DB01151; Desipramine.
DR DrugBank; DB00804; Dicyclomine.
DR DrugBank; DB01231; Diphenidol.
DR DrugBank; DB00280; Disopyramide.
DR DrugBank; DB09167; Dosulepin.
DR DrugBank; DB01142; Doxepin.
DR DrugBank; DB00366; Doxylamine.
DR DrugBank; DB01175; Escitalopram.
DR DrugBank; DB09194; Etoperidone.
DR DrugBank; DB06702; Fesoterodine.
DR DrugBank; DB01148; Flavoxate.
DR DrugBank; DB00875; Flupentixol.
DR DrugBank; DB00483; Gallamine triethiodide.
DR DrugBank; DB00986; Glycopyrronium.
DR DrugBank; DB06787; Hexocyclium.
DR DrugBank; DB11181; Homatropine.
DR DrugBank; DB00725; Homatropine methylbromide.
DR DrugBank; DB00424; Hyoscyamine.
DR DrugBank; DB09262; Imidafenacin.
DR DrugBank; DB00458; Imipramine.
DR DrugBank; DB00332; Ipratropium.
DR DrugBank; DB01221; Ketamine.
DR DrugBank; DB00408; Loxapine.
DR DrugBank; DB00934; Maprotiline.
DR DrugBank; DB04843; Mepenzolate.
DR DrugBank; DB00454; Meperidine.
DR DrugBank; DB06709; Methacholine.
DR DrugBank; DB00940; Methantheline.
DR DrugBank; DB01403; Methotrimeprazine.
DR DrugBank; DB00462; Methscopolamine bromide.
DR DrugBank; DB00340; Metixene.
DR DrugBank; DB01233; Metoclopramide.
DR DrugBank; DB00805; Minaprine.
DR DrugBank; DB01618; Molindone.
DR DrugBank; DB05152; NGX267.
DR DrugBank; DB00622; Nicardipine.
DR DrugBank; DB00540; Nortriptyline.
DR DrugBank; DB00334; Olanzapine.
DR DrugBank; DB01062; Oxybutynin.
DR DrugBank; DB00383; Oxyphencyclimine.
DR DrugBank; DB00219; Oxyphenonium.
DR DrugBank; DB00715; Paroxetine.
DR DrugBank; DB01085; Pilocarpine.
DR DrugBank; DB00670; Pirenzepine.
DR DrugBank; DB06153; Pizotifen.
DR DrugBank; DB00387; Procyclidine.
DR DrugBank; DB00392; Profenamine.
DR DrugBank; DB00420; Promazine.
DR DrugBank; DB01069; Promethazine.
DR DrugBank; DB00782; Propantheline.
DR DrugBank; DB00777; Propiomazine.
DR DrugBank; DB12278; Propiverine.
DR DrugBank; DB11156; Pyrantel.
DR DrugBank; DB01224; Quetiapine.
DR DrugBank; DB11855; Revefenacin.
DR DrugBank; DB13581; Rociverine.
DR DrugBank; DB00747; Scopolamine.
DR DrugBank; DB01591; Solifenacin.
DR DrugBank; DB02010; Staurosporine.
DR DrugBank; DB00342; Terfenadine.
DR DrugBank; DB11235; Thonzylamine.
DR DrugBank; DB01409; Tiotropium.
DR DrugBank; DB01036; Tolterodine.
DR DrugBank; DB00193; Tramadol.
DR DrugBank; DB00505; Tridihexethyl.
DR DrugBank; DB00508; Triflupromazine.
DR DrugBank; DB00376; Trihexyphenidyl.
DR DrugBank; DB09089; Trimebutine.
DR DrugBank; DB00726; Trimipramine.
DR DrugBank; DB00809; Tropicamide.
DR DrugBank; DB00209; Trospium.
DR DrugBank; DB09076; Umeclidinium.
DR DrugBank; DB00246; Ziprasidone.
DR DrugCentral; P11229; -.
DR GuidetoPHARMACOLOGY; 13; -.
DR GlyGen; P11229; 2 sites.
DR iPTMnet; P11229; -.
DR PhosphoSitePlus; P11229; -.
DR BioMuta; CHRM1; -.
DR DMDM; 113118; -.
DR MassIVE; P11229; -.
DR PaxDb; P11229; -.
DR PeptideAtlas; P11229; -.
DR PRIDE; P11229; -.
DR ProteomicsDB; 52724; -. [P11229-1]
DR ProteomicsDB; 77961; -.
DR ABCD; P11229; 12 sequenced antibodies.
DR Antibodypedia; 2948; 326 antibodies from 37 providers.
DR DNASU; 1128; -.
DR Ensembl; ENST00000306960.4; ENSP00000306490.3; ENSG00000168539.4. [P11229-1]
DR Ensembl; ENST00000543973.1; ENSP00000441188.1; ENSG00000168539.4. [P11229-2]
DR GeneID; 1128; -.
DR KEGG; hsa:1128; -.
DR MANE-Select; ENST00000306960.4; ENSP00000306490.3; NM_000738.3; NP_000729.2.
DR UCSC; uc058cqg.1; human. [P11229-1]
DR CTD; 1128; -.
DR DisGeNET; 1128; -.
DR GeneCards; CHRM1; -.
DR HGNC; HGNC:1950; CHRM1.
DR HPA; ENSG00000168539; Group enriched (brain, prostate, salivary gland).
DR MIM; 118510; gene.
DR neXtProt; NX_P11229; -.
DR OpenTargets; ENSG00000168539; -.
DR PharmGKB; PA26484; -.
DR VEuPathDB; HostDB:ENSG00000168539; -.
DR eggNOG; KOG4220; Eukaryota.
DR GeneTree; ENSGT00940000162301; -.
DR HOGENOM; CLU_009579_11_2_1; -.
DR InParanoid; P11229; -.
DR OMA; RCCRTPR; -.
DR PhylomeDB; P11229; -.
DR TreeFam; TF320495; -.
DR PathwayCommons; P11229; -.
DR Reactome; R-HSA-390648; Muscarinic acetylcholine receptors.
DR Reactome; R-HSA-416476; G alpha (q) signalling events.
DR SignaLink; P11229; -.
DR SIGNOR; P11229; -.
DR BioGRID-ORCS; 1128; 15 hits in 1073 CRISPR screens.
DR ChiTaRS; CHRM1; human.
DR GeneWiki; Muscarinic_acetylcholine_receptor_M1; -.
DR GenomeRNAi; 1128; -.
DR Pharos; P11229; Tclin.
DR PRO; PR:P11229; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; P11229; protein.
DR Bgee; ENSG00000168539; Expressed in prefrontal cortex and 91 other tissues.
DR ExpressionAtlas; P11229; baseline and differential.
DR Genevisible; P11229; HS.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0043679; C:axon terminus; IEA:Ensembl.
DR GO; GO:0098981; C:cholinergic synapse; IEA:Ensembl.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0098978; C:glutamatergic synapse; IEA:Ensembl.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099061; C:integral component of postsynaptic density membrane; IEA:Ensembl.
DR GO; GO:0099056; C:integral component of presynaptic membrane; IEA:Ensembl.
DR GO; GO:0016020; C:membrane; TAS:ProtInc.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0098685; C:Schaffer collateral - CA1 synapse; IEA:Ensembl.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0016907; F:G protein-coupled acetylcholine receptor activity; IBA:GO_Central.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0099529; F:neurotransmitter receptor activity involved in regulation of postsynaptic membrane potential; IEA:Ensembl.
DR GO; GO:0004435; F:phosphatidylinositol phospholipase C activity; TAS:ProtInc.
DR GO; GO:0007197; P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0050890; P:cognition; IEA:InterPro.
DR GO; GO:0007213; P:G protein-coupled acetylcholine receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0007399; P:nervous system development; TAS:ProtInc.
DR GO; GO:0007274; P:neuromuscular synaptic transmission; IEA:Ensembl.
DR GO; GO:0007207; P:phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0090316; P:positive regulation of intracellular protein transport; IEA:Ensembl.
DR GO; GO:0043270; P:positive regulation of ion transport; IGI:MGI.
DR GO; GO:0007205; P:protein kinase C-activating G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0060251; P:regulation of glial cell proliferation; TAS:GO_Central.
DR GO; GO:0040012; P:regulation of locomotion; IEA:Ensembl.
DR GO; GO:0046541; P:saliva secretion; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; TAS:GO_Central.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR002228; Musac_Ach_M1_rcpt.
DR InterPro; IPR000995; Musac_Ach_rcpt.
DR PANTHER; PTHR24248:SF155; PTHR24248:SF155; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00243; MUSCARINICR.
DR PRINTS; PR00538; MUSCRINICM1R.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cell membrane; Disulfide bond;
KW G-protein coupled receptor; Glycoprotein; Membrane; Phosphoprotein;
KW Postsynaptic cell membrane; Receptor; Reference proteome; Synapse;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..460
FT /note="Muscarinic acetylcholine receptor M1"
FT /id="PRO_0000069015"
FT TOPO_DOM 1..22
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TRANSMEM 23..48
FT /note="Helical; Name=1"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TOPO_DOM 49..62
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TRANSMEM 63..84
FT /note="Helical; Name=2"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TOPO_DOM 85..95
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TRANSMEM 96..121
FT /note="Helical; Name=3"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TOPO_DOM 122..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TRANSMEM 143..164
FT /note="Helical; Name=4"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TOPO_DOM 165..185
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TRANSMEM 186..209
FT /note="Helical; Name=5"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TOPO_DOM 210..366
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TRANSMEM 367..390
FT /note="Helical; Name=6"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TOPO_DOM 391..397
FT /note="Extracellular"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TRANSMEM 398..420
FT /note="Helical; Name=7"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT TOPO_DOM 421..460
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:32646996,
FT ECO:0007744|PDB:6WJC"
FT REGION 225..256
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 274..297
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 310..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 231..248
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 170
FT /note="Subtype-specific residue that binds to snake venom
FT muscarinic toxin 7"
FT /evidence="ECO:0000269|PubMed:32646996"
FT SITE 172
FT /note="Binds to snake venom muscarinic toxin 7"
FT /evidence="ECO:0000269|PubMed:32646996"
FT SITE 174
FT /note="Subtype-specific residue that binds to snake venom
FT muscarinic toxin 7"
FT /evidence="ECO:0000269|PubMed:32646996"
FT SITE 397
FT /note="Subtype-specific residue that binds to snake venom
FT muscarinic toxin 7"
FT /evidence="ECO:0000269|PubMed:32646996"
FT SITE 401
FT /note="Subtype-specific residue that binds to snake venom
FT muscarinic toxin 7"
FT /evidence="ECO:0000269|PubMed:32646996"
FT MOD_RES 230
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:P12657"
FT MOD_RES 428
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 451
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT MOD_RES 455
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 457
FT /note="Phosphoserine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 12
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 98..178
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VAR_SEQ 455..460
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_056651"
FT CONFLICT 173
FT /note="V -> M (in Ref. 3; CAA33334)"
FT /evidence="ECO:0000305"
FT HELIX 27..52
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 59..76
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 78..88
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 95..128
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT TURN 130..132
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 133..135
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 139..168
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 182..184
FT /evidence="ECO:0007829|PDB:6ZG4"
FT HELIX 186..196
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 198..219
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 362..390
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT STRAND 391..393
FT /evidence="ECO:0007829|PDB:6WJC"
FT HELIX 397..408
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 410..421
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 423..437
FT /evidence="ECO:0007829|PDB:6ZFZ"
FT HELIX 439..441
FT /evidence="ECO:0007829|PDB:6OIJ"
SQ SEQUENCE 460 AA; 51421 MW; 567C20F63541C8D0 CRC64;
MNTSAPPAVS PNITVLAPGK GPWQVAFIGI TTGLLSLATV TGNLLVLISF KVNTELKTVN
NYFLLSLACA DLIIGTFSMN LYTTYLLMGH WALGTLACDL WLALDYVASN ASVMNLLLIS
FDRYFSVTRP LSYRAKRTPR RAALMIGLAW LVSFVLWAPA ILFWQYLVGE RTVLAGQCYI
QFLSQPIITF GTAMAAFYLP VTVMCTLYWR IYRETENRAR ELAALQGSET PGKGGGSSSS
SERSQPGAEG SPETPPGRCC RCCRAPRLLQ AYSWKEEEEE DEGSMESLTS SEGEEPGSEV
VIKMPMVDPE AQAPTKQPPR SSPNTVKRPT KKGRDRAGKG QKPRGKEQLA KRKTFSLVKE
KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STINPMCYAL
CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC
