ACM2_HUMAN
ID ACM2_HUMAN Reviewed; 466 AA.
AC P08172; Q4VBK6; Q9P1X9;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 212.
DE RecName: Full=Muscarinic acetylcholine receptor M2;
GN Name=CHRM2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3037705; DOI=10.1126/science.3037705;
RA Bonner T.I., Buckley N.J., Young A.C., Brann M.R.;
RT "Identification of a family of muscarinic acetylcholine receptor genes.";
RL Science 237:527-532(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=3443095; DOI=10.1002/j.1460-2075.1987.tb02733.x;
RA Peralta E.G., Ashkenazi A., Winslow J.W., Smith D.H., Ramachandran J.,
RA Capon D.J.;
RT "Distinct primary structures, ligand-binding properties and tissue-specific
RT expression of four human muscarinic acetylcholine receptors.";
RL EMBO J. 6:3923-3929(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 27-466.
RX PubMed=15014171; DOI=10.1093/molbev/msh100;
RA Kitano T., Liu Y.-H., Ueda S., Saitou N.;
RT "Human-specific amino acid changes found in 103 protein-coding genes.";
RL Mol. Biol. Evol. 21:936-944(2004).
RN [6]
RP INTERACTION WITH ARRB1 AND ARRB2.
RX PubMed=7822302; DOI=10.1074/jbc.270.2.720;
RA Gurevich V.V., Dion S.B., Onorato J.J., Ptasienski J., Kim C.M.,
RA Sterne-Marr R., Hosey M.M., Benovic J.L.;
RT "Arrestin interactions with G protein-coupled receptors. Direct binding
RT studies of wild type and mutant arrestins with rhodopsin, beta 2-
RT adrenergic, and m2 muscarinic cholinergic receptors.";
RL J. Biol. Chem. 270:720-731(1995).
RN [7]
RP INDUCTION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=16548883; DOI=10.1111/j.1462-5822.2005.00644.x;
RA Leong W.F., Chow V.T.;
RT "Transcriptomic and proteomic analyses of rhabdomyosarcoma cells reveal
RT differential cellular gene expression in response to enterovirus 71
RT infection.";
RL Cell. Microbiol. 8:565-580(2006).
RN [8]
RP INVOLVEMENT IN SUSCEPTIBILITY TO ALCOHOLISM AND MAJOR DEPRESSIVE DISORDER.
RX PubMed=15229186; DOI=10.1093/hmg/ddh194;
RA Wang J.C., Hinrichs A.L., Stock H., Budde J., Allen R., Bertelsen S.,
RA Kwon J.M., Wu W., Dick D.M., Rice J., Jones K., Nurnberger J.I. Jr.,
RA Tischfield J., Porjesz B., Edenberg H.J., Hesselbrock V., Crowe R.,
RA Schuckit M., Begleiter H., Reich T., Goate A.M., Bierut L.J.;
RT "Evidence of common and specific genetic effects: association of the
RT muscarinic acetylcholine receptor M2 (CHRM2) gene with alcohol dependence
RT and major depressive syndrome.";
RL Hum. Mol. Genet. 13:1903-1911(2004).
RN [9]
RP INTERACTION WITH RACK1.
RX PubMed=20976005; DOI=10.1371/journal.pone.0013517;
RA Reiner C.L., McCullar J.S., Kow R.L., Le J.H., Goodlett D.R.,
RA Nathanson N.M.;
RT "RACK1 associates with muscarinic receptors and regulates M(2) receptor
RT trafficking.";
RL PLoS ONE 5:E13517-E13517(2010).
RN [10]
RP X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) IN COMPLEX WITH THE ANTAGONIST
RP 3-QUINUCLIDINYLBENZILATE, SUBCELLULAR LOCATION, TOPOLOGY, AND DISULFIDE
RP BOND.
RX PubMed=22278061; DOI=10.1038/nature10753;
RA Haga K., Kruse A.C., Asada H., Yurugi-Kobayashi T., Shiroishi M., Zhang C.,
RA Weis W.I., Okada T., Kobilka B.K., Haga T., Kobayashi T.;
RT "Structure of the human M2 muscarinic acetylcholine receptor bound to an
RT antagonist.";
RL Nature 482:547-551(2012).
RN [11]
RP X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) IN COMPLEXES WITH THE AGONIST
RP IPEROXO AND THE ALLOSTERIC EFFECTOR LY2119620, FUNCTION, SUBCELLULAR
RP LOCATION, TOPOLOGY, DISULFIDE BOND, MOTIF, AND MUTAGENESIS OF ASN-58;
RP ASP-103; TYR-206 AND ASN-404.
RX PubMed=24256733; DOI=10.1038/nature12735;
RA Kruse A.C., Ring A.M., Manglik A., Hu J., Hu K., Eitel K., Hubner H.,
RA Pardon E., Valant C., Sexton P.M., Christopoulos A., Felder C.C.,
RA Gmeiner P., Steyaert J., Weis W.I., Garcia K.C., Wess J., Kobilka B.K.;
RT "Activation and allosteric modulation of a muscarinic acetylcholine
RT receptor.";
RL Nature 504:101-106(2013).
CC -!- FUNCTION: The muscarinic acetylcholine receptor mediates various
CC cellular responses, including inhibition of adenylate cyclase,
CC breakdown of phosphoinositides and modulation of potassium channels
CC through the action of G proteins. Primary transducing effect is
CC adenylate cyclase inhibition. Signaling promotes phospholipase C
CC activity, leading to the release of inositol trisphosphate (IP3); this
CC then triggers calcium ion release into the cytosol.
CC {ECO:0000269|PubMed:24256733, ECO:0000269|PubMed:3443095}.
CC -!- SUBUNIT: Interacts with ARRB1 and ARRB2. Interacts with RACK1; the
CC interaction regulates CHRM2 internalization.
CC {ECO:0000269|PubMed:20976005, ECO:0000269|PubMed:22278061,
CC ECO:0000269|PubMed:7822302}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22278061,
CC ECO:0000269|PubMed:24256733, ECO:0000269|PubMed:3443095}; Multi-pass
CC membrane protein {ECO:0000269|PubMed:22278061,
CC ECO:0000269|PubMed:24256733}. Postsynaptic cell membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}. Note=Phosphorylation in
CC response to agonist binding promotes receptor internalization.
CC {ECO:0000250|UniProtKB:P06199}.
CC -!- INDUCTION: Up-regulated in response to enterovirus 71 (EV71) infection.
CC {ECO:0000269|PubMed:16548883}.
CC -!- PTM: Phosphorylated in response to agonist treatment. {ECO:0000250}.
CC -!- POLYMORPHISM: Genetic variations in CHRM2 can influence susceptibility
CC to alcoholism [MIM:103780]. {ECO:0000305|PubMed:15229186}.
CC -!- DISEASE: Major depressive disorder (MDD) [MIM:608516]: A common
CC psychiatric disorder. It is a complex trait characterized by one or
CC more major depressive episodes without a history of manic, mixed, or
CC hypomanic episodes. A major depressive episode is characterized by at
CC least 2 weeks during which there is a new onset or clear worsening of
CC either depressed mood or loss of interest or pleasure in nearly all
CC activities. Four additional symptoms must also be present including
CC changes in appetite, weight, sleep, and psychomotor activity; decreased
CC energy; feelings of worthlessness or guilt; difficulty thinking,
CC concentrating, or making decisions; or recurrent thoughts of death or
CC suicidal ideation, plans, or attempts. The episode must be accompanied
CC by distress or impairment in social, occupational, or other important
CC areas of functioning. {ECO:0000269|PubMed:15229186}. Note=Disease
CC susceptibility is associated with variants affecting the gene
CC represented in this entry.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; M16404; AAA51570.1; -; Genomic_DNA.
DR EMBL; X15264; CAA33335.1; -; Genomic_DNA.
DR EMBL; AF498916; AAM18939.1; -; mRNA.
DR EMBL; BC095547; AAH95547.1; -; mRNA.
DR EMBL; BC106741; AAI06742.1; -; mRNA.
DR EMBL; BC106742; AAI06743.1; -; mRNA.
DR EMBL; AB041391; BAA94476.1; -; Genomic_DNA.
DR CCDS; CCDS5843.1; -.
DR PIR; S10126; S10126.
DR RefSeq; NP_000730.1; NM_000739.2.
DR RefSeq; NP_001006627.1; NM_001006626.1.
DR RefSeq; NP_001006628.1; NM_001006627.1.
DR RefSeq; NP_001006629.1; NM_001006628.1.
DR RefSeq; NP_001006630.1; NM_001006629.1.
DR RefSeq; NP_001006631.1; NM_001006630.1.
DR RefSeq; NP_001006632.1; NM_001006631.1.
DR RefSeq; NP_001006633.1; NM_001006632.1.
DR RefSeq; XP_011514071.1; XM_011515769.2.
DR PDB; 3UON; X-ray; 3.00 A; A=1-217, A=377-466.
DR PDB; 4MQS; X-ray; 3.50 A; A=1-466.
DR PDB; 4MQT; X-ray; 3.70 A; A=1-466.
DR PDB; 5YC8; X-ray; 2.50 A; A=10-466.
DR PDB; 5ZK3; X-ray; 2.60 A; A=10-466.
DR PDB; 5ZK8; X-ray; 3.00 A; A=10-466.
DR PDB; 5ZKB; X-ray; 2.95 A; A=10-466.
DR PDB; 5ZKC; X-ray; 2.30 A; A=10-466.
DR PDB; 6OIK; EM; 3.60 A; R=3-466.
DR PDB; 6U1N; EM; 4.00 A; R=2-466.
DR PDBsum; 3UON; -.
DR PDBsum; 4MQS; -.
DR PDBsum; 4MQT; -.
DR PDBsum; 5YC8; -.
DR PDBsum; 5ZK3; -.
DR PDBsum; 5ZK8; -.
DR PDBsum; 5ZKB; -.
DR PDBsum; 5ZKC; -.
DR PDBsum; 6OIK; -.
DR PDBsum; 6U1N; -.
DR AlphaFoldDB; P08172; -.
DR SMR; P08172; -.
DR BioGRID; 107551; 19.
DR DIP; DIP-56282N; -.
DR IntAct; P08172; 23.
DR MINT; P08172; -.
DR STRING; 9606.ENSP00000399745; -.
DR BindingDB; P08172; -.
DR ChEMBL; CHEMBL211; -.
DR DrugBank; DB03128; Acetylcholine.
DR DrugBank; DB08897; Aclidinium.
DR DrugBank; DB05752; ALKS 27.
DR DrugBank; DB00321; Amitriptyline.
DR DrugBank; DB00543; Amoxapine.
DR DrugBank; DB00517; Anisotropine methylbromide.
DR DrugBank; DB01238; Aripiprazole.
DR DrugBank; DB14185; Aripiprazole lauroxil.
DR DrugBank; DB00572; Atropine.
DR DrugBank; DB00767; Benzquinamide.
DR DrugBank; DB01019; Bethanechol.
DR DrugBank; DB00835; Brompheniramine.
DR DrugBank; DB09300; Butylscopolamine.
DR DrugBank; DB00411; Carbamoylcholine.
DR DrugBank; DB01239; Chlorprothixene.
DR DrugBank; DB00568; Cinnarizine.
DR DrugBank; DB00363; Clozapine.
DR DrugBank; DB00907; Cocaine.
DR DrugBank; DB00785; Cryptenamine.
DR DrugBank; DB00434; Cyproheptadine.
DR DrugBank; DB00496; Darifenacin.
DR DrugBank; DB01151; Desipramine.
DR DrugBank; DB00804; Dicyclomine.
DR DrugBank; DB08801; Dimetindene.
DR DrugBank; DB01075; Diphenhydramine.
DR DrugBank; DB01231; Diphenidol.
DR DrugBank; DB00280; Disopyramide.
DR DrugBank; DB09167; Dosulepin.
DR DrugBank; DB01135; Doxacurium.
DR DrugBank; DB01142; Doxepin.
DR DrugBank; DB00366; Doxylamine.
DR DrugBank; DB09194; Etoperidone.
DR DrugBank; DB06702; Fesoterodine.
DR DrugBank; DB01148; Flavoxate.
DR DrugBank; DB00483; Gallamine triethiodide.
DR DrugBank; DB00986; Glycopyrronium.
DR DrugBank; DB06787; Hexocyclium.
DR DrugBank; DB11181; Homatropine.
DR DrugBank; DB00725; Homatropine methylbromide.
DR DrugBank; DB00424; Hyoscyamine.
DR DrugBank; DB09262; Imidafenacin.
DR DrugBank; DB00458; Imipramine.
DR DrugBank; DB00332; Ipratropium.
DR DrugBank; DB01221; Ketamine.
DR DrugBank; DB00408; Loxapine.
DR DrugBank; DB00934; Maprotiline.
DR DrugBank; DB00454; Meperidine.
DR DrugBank; DB06709; Methacholine.
DR DrugBank; DB00940; Methantheline.
DR DrugBank; DB01403; Methotrimeprazine.
DR DrugBank; DB00462; Methscopolamine bromide.
DR DrugBank; DB00340; Metixene.
DR DrugBank; DB01336; Metocurine.
DR DrugBank; DB01226; Mivacurium.
DR DrugBank; DB00622; Nicardipine.
DR DrugBank; DB00540; Nortriptyline.
DR DrugBank; DB00334; Olanzapine.
DR DrugBank; DB01062; Oxybutynin.
DR DrugBank; DB00383; Oxyphencyclimine.
DR DrugBank; DB01337; Pancuronium.
DR DrugBank; DB00715; Paroxetine.
DR DrugBank; DB01085; Pilocarpine.
DR DrugBank; DB01338; Pipecuronium.
DR DrugBank; DB06153; Pizotifen.
DR DrugBank; DB00387; Procyclidine.
DR DrugBank; DB00392; Profenamine.
DR DrugBank; DB01069; Promethazine.
DR DrugBank; DB00777; Propiomazine.
DR DrugBank; DB12278; Propiverine.
DR DrugBank; DB01224; Quetiapine.
DR DrugBank; DB04834; Rapacuronium.
DR DrugBank; DB11855; Revefenacin.
DR DrugBank; DB13581; Rociverine.
DR DrugBank; DB00728; Rocuronium.
DR DrugBank; DB00747; Scopolamine.
DR DrugBank; DB01591; Solifenacin.
DR DrugBank; DB00202; Succinylcholine.
DR DrugBank; DB00342; Terfenadine.
DR DrugBank; DB11235; Thonzylamine.
DR DrugBank; DB01409; Tiotropium.
DR DrugBank; DB01036; Tolterodine.
DR DrugBank; DB00505; Tridihexethyl.
DR DrugBank; DB00508; Triflupromazine.
DR DrugBank; DB00376; Trihexyphenidyl.
DR DrugBank; DB09089; Trimebutine.
DR DrugBank; DB00726; Trimipramine.
DR DrugBank; DB00809; Tropicamide.
DR DrugBank; DB09076; Umeclidinium.
DR DrugBank; DB00246; Ziprasidone.
DR DrugCentral; P08172; -.
DR GuidetoPHARMACOLOGY; 14; -.
DR GlyGen; P08172; 4 sites.
DR iPTMnet; P08172; -.
DR PhosphoSitePlus; P08172; -.
DR SwissPalm; P08172; -.
DR BioMuta; CHRM2; -.
DR DMDM; 113122; -.
DR MassIVE; P08172; -.
DR PaxDb; P08172; -.
DR PeptideAtlas; P08172; -.
DR PRIDE; P08172; -.
DR ProteomicsDB; 52076; -.
DR ABCD; P08172; 3 sequenced antibodies.
DR Antibodypedia; 18171; 566 antibodies from 39 providers.
DR DNASU; 1129; -.
DR Ensembl; ENST00000320658.9; ENSP00000319984.5; ENSG00000181072.12.
DR Ensembl; ENST00000401861.1; ENSP00000384401.1; ENSG00000181072.12.
DR Ensembl; ENST00000445907.6; ENSP00000399745.2; ENSG00000181072.12.
DR Ensembl; ENST00000453373.5; ENSP00000415386.1; ENSG00000181072.12.
DR Ensembl; ENST00000680005.1; ENSP00000505686.1; ENSG00000181072.12.
DR GeneID; 1129; -.
DR KEGG; hsa:1129; -.
DR MANE-Select; ENST00000680005.1; ENSP00000505686.1; NM_001006630.2; NP_001006631.1.
DR UCSC; uc003vtg.2; human.
DR CTD; 1129; -.
DR DisGeNET; 1129; -.
DR GeneCards; CHRM2; -.
DR HGNC; HGNC:1951; CHRM2.
DR HPA; ENSG00000181072; Tissue enhanced (heart muscle, intestine).
DR MIM; 103780; phenotype.
DR MIM; 118493; gene.
DR MIM; 608516; phenotype.
DR neXtProt; NX_P08172; -.
DR OpenTargets; ENSG00000181072; -.
DR PharmGKB; PA111; -.
DR VEuPathDB; HostDB:ENSG00000181072; -.
DR eggNOG; KOG4220; Eukaryota.
DR GeneTree; ENSGT00940000158940; -.
DR HOGENOM; CLU_009579_11_2_1; -.
DR InParanoid; P08172; -.
DR OMA; TSERQNH; -.
DR OrthoDB; 1025450at2759; -.
DR PhylomeDB; P08172; -.
DR TreeFam; TF320495; -.
DR PathwayCommons; P08172; -.
DR Reactome; R-HSA-390648; Muscarinic acetylcholine receptors.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR Reactome; R-HSA-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-HSA-8856828; Clathrin-mediated endocytosis.
DR SignaLink; P08172; -.
DR SIGNOR; P08172; -.
DR BioGRID-ORCS; 1129; 12 hits in 1067 CRISPR screens.
DR ChiTaRS; CHRM2; human.
DR GeneWiki; Muscarinic_acetylcholine_receptor_M2; -.
DR GenomeRNAi; 1129; -.
DR Pharos; P08172; Tclin.
DR PRO; PR:P08172; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; P08172; protein.
DR Bgee; ENSG00000181072; Expressed in stromal cell of endometrium and 69 other tissues.
DR ExpressionAtlas; P08172; baseline and differential.
DR Genevisible; P08172; HS.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0032279; C:asymmetric synapse; IEA:Ensembl.
DR GO; GO:0043679; C:axon terminus; IEA:Ensembl.
DR GO; GO:0098981; C:cholinergic synapse; IEA:Ensembl.
DR GO; GO:0030669; C:clathrin-coated endocytic vesicle membrane; TAS:Reactome.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0098978; C:glutamatergic synapse; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; IDA:CAFA.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:UniProtKB.
DR GO; GO:0099055; C:integral component of postsynaptic membrane; IEA:Ensembl.
DR GO; GO:0099056; C:integral component of presynaptic membrane; IEA:Ensembl.
DR GO; GO:0043025; C:neuronal cell body; IEA:Ensembl.
DR GO; GO:0005886; C:plasma membrane; IDA:LIFEdb.
DR GO; GO:0032280; C:symmetric synapse; IEA:Ensembl.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:1990763; F:arrestin family protein binding; IPI:CAFA.
DR GO; GO:0016907; F:G protein-coupled acetylcholine receptor activity; IDA:UniProtKB.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0007197; P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0007213; P:G protein-coupled acetylcholine receptor signaling pathway; IDA:UniProtKB.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0007399; P:nervous system development; TAS:ProtInc.
DR GO; GO:0007207; P:phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0008016; P:regulation of heart contraction; TAS:ProtInc.
DR GO; GO:0006940; P:regulation of smooth muscle contraction; IBA:GO_Central.
DR GO; GO:2000300; P:regulation of synaptic vesicle exocytosis; IEA:Ensembl.
DR GO; GO:0009615; P:response to virus; IEP:UniProtKB.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001065; Musac_Ach_M2_rcpt.
DR InterPro; IPR000995; Musac_Ach_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00243; MUSCARINICR.
DR PRINTS; PR00539; MUSCRINICM2R.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Membrane; Phosphoprotein; Postsynaptic cell membrane;
KW Receptor; Reference proteome; Synapse; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..466
FT /note="Muscarinic acetylcholine receptor M2"
FT /id="PRO_0000069021"
FT TOPO_DOM 1..22
FT /note="Extracellular"
FT TRANSMEM 23..45
FT /note="Helical; Name=1"
FT TOPO_DOM 46..59
FT /note="Cytoplasmic"
FT TRANSMEM 60..80
FT /note="Helical; Name=2"
FT TOPO_DOM 81..97
FT /note="Extracellular"
FT TRANSMEM 98..119
FT /note="Helical; Name=3"
FT TOPO_DOM 120..139
FT /note="Cytoplasmic"
FT TRANSMEM 140..162
FT /note="Helical; Name=4"
FT TOPO_DOM 163..184
FT /note="Extracellular"
FT TRANSMEM 185..209
FT /note="Helical; Name=5"
FT TOPO_DOM 210..387
FT /note="Cytoplasmic"
FT TRANSMEM 388..410
FT /note="Helical; Name=6"
FT TOPO_DOM 411..418
FT /note="Extracellular"
FT TRANSMEM 419..442
FT /note="Helical; Name=7"
FT TOPO_DOM 443..466
FT /note="Cytoplasmic"
FT REGION 218..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 120..122
FT /note="Important for signaling"
FT MOTIF 436..440
FT /note="Important for signaling"
FT COMPBIAS 230..256
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 280..297
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 305..355
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 232
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ERZ4"
FT MOD_RES 446
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 450
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 465
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 2
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 3
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 6
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 9
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 96..176
FT DISULFID 413..416
FT MUTAGEN 58
FT /note="N->A: Nearly abolishes signaling via downstream
FT effectors."
FT /evidence="ECO:0000269|PubMed:24256733"
FT MUTAGEN 103
FT /note="D->E: Reduced affinity for acetylcholine. Abolishes
FT signaling via downstream effectors."
FT /evidence="ECO:0000269|PubMed:24256733"
FT MUTAGEN 206
FT /note="Y->F: Abolishes signaling via downstream effectors."
FT /evidence="ECO:0000269|PubMed:24256733"
FT MUTAGEN 404
FT /note="N->Q: Reduced affinity for acetylcholine. Reduces
FT signaling via downstream effectors."
FT /evidence="ECO:0000269|PubMed:24256733"
FT HELIX 17..50
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 52..54
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 57..74
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 76..86
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 93..126
FT /evidence="ECO:0007829|PDB:5ZKC"
FT TURN 128..131
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 132..134
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 137..166
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 179..182
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 184..194
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 196..213
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 382..410
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 414..416
FT /evidence="ECO:0007829|PDB:5YC8"
FT HELIX 419..440
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 441..443
FT /evidence="ECO:0007829|PDB:5ZKC"
FT HELIX 445..455
FT /evidence="ECO:0007829|PDB:5ZKC"
SQ SEQUENCE 466 AA; 51715 MW; 2FC2FD7748C22BEC CRC64;
MNNSTNSSNN SLALTSPYKT FEVVFIVLVA GSLSLVTIIG NILVMVSIKV NRHLQTVNNY
FLFSLACADL IIGVFSMNLY TLYTVIGYWP LGPVVCDLWL ALDYVVSNAS VMNLLIISFD
RYFCVTKPLT YPVKRTTKMA GMMIAAAWVL SFILWAPAIL FWQFIVGVRT VEDGECYIQF
FSNAAVTFGT AIAAFYLPVI IMTVLYWHIS RASKSRIKKD KKEPVANQDP VSPSLVQGRI
VKPNNNNMPS SDDGLEHNKI QNGKAPRDPV TENCVQGEEK ESSNDSTSVS AVASNMRDDE
ITQDENTVST SLGHSKDENS KQTCIRIGTK TPKSDSCTPT NTTVEVVGSS GQNGDEKQNI
VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW APYNVMVLIN TFCAPCIPNT
VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR
