ACM4_RAT
ID ACM4_RAT Reviewed; 478 AA.
AC P08485;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Muscarinic acetylcholine receptor M4;
GN Name=Chrm4; Synonyms=Chrm-4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3037705; DOI=10.1126/science.3037705;
RA Bonner T.I., Buckley N.J., Young A.C., Brann M.R.;
RT "Identification of a family of muscarinic acetylcholine receptor genes.";
RL Science 237:527-532(1987).
CC -!- FUNCTION: The muscarinic acetylcholine receptor mediates various
CC cellular responses, including inhibition of adenylate cyclase,
CC breakdown of phosphoinositides and modulation of potassium channels
CC through the action of G proteins. Primary transducing effect is
CC inhibition of adenylate cyclase.
CC -!- INTERACTION:
CC P08485; P11275: Camk2a; NbExp=10; IntAct=EBI-7946147, EBI-2640645;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Postsynaptic cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; M16409; AAA40663.1; ALT_SEQ; mRNA.
DR PIR; C94518; C29514.
DR RefSeq; NP_113735.1; NM_031547.1.
DR IntAct; P08485; 1.
DR MINT; P08485; -.
DR STRING; 10116.ENSRNOP00000023586; -.
DR BindingDB; P08485; -.
DR ChEMBL; CHEMBL317; -.
DR DrugCentral; P08485; -.
DR GuidetoPHARMACOLOGY; 16; -.
DR GlyGen; P08485; 2 sites.
DR iPTMnet; P08485; -.
DR PhosphoSitePlus; P08485; -.
DR PaxDb; P08485; -.
DR GeneID; 25111; -.
DR KEGG; rno:25111; -.
DR UCSC; RGD:2344; rat.
DR CTD; 1132; -.
DR RGD; 2344; Chrm4.
DR eggNOG; KOG4220; Eukaryota.
DR InParanoid; P08485; -.
DR OrthoDB; 1245472at2759; -.
DR PhylomeDB; P08485; -.
DR Reactome; R-RNO-390648; Muscarinic acetylcholine receptors.
DR Reactome; R-RNO-418594; G alpha (i) signalling events.
DR PRO; PR:P08485; -.
DR Proteomes; UP000002494; Unplaced.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0032279; C:asymmetric synapse; IDA:RGD.
DR GO; GO:0043679; C:axon terminus; IDA:RGD.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099061; C:integral component of postsynaptic density membrane; IDA:SynGO.
DR GO; GO:0099056; C:integral component of presynaptic membrane; IDA:SynGO.
DR GO; GO:0016020; C:membrane; IDA:RGD.
DR GO; GO:0043025; C:neuronal cell body; IDA:RGD.
DR GO; GO:0014069; C:postsynaptic density; IDA:RGD.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0016907; F:G protein-coupled acetylcholine receptor activity; ISO:RGD.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:RGD.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0007197; P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; ISO:RGD.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0040012; P:regulation of locomotion; IEA:InterPro.
DR GO; GO:0010033; P:response to organic substance; IEP:RGD.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR001432; Musac_Ach_M4_rcpt.
DR InterPro; IPR000995; Musac_Ach_rcpt.
DR PANTHER; PTHR24248:SF142; PTHR24248:SF142; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00243; MUSCARINICR.
DR PRINTS; PR00541; MUSCRINICM4R.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Postsynaptic cell membrane; Receptor;
KW Reference proteome; Synapse; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..478
FT /note="Muscarinic acetylcholine receptor M4"
FT /id="PRO_0000069039"
FT TOPO_DOM 1..30
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 31..53
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 54..67
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 68..88
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 89..105
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 106..127
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 128..147
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 148..170
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 171..192
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 193..215
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 216..400
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 401..421
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 422..435
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 436..455
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 456..478
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 249..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..288
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 289..333
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 458
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 462
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 476
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 13
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 104..184
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 478 AA; 52921 MW; 2551FF5F77B1A56C CRC64;
MXNFTPVNGS SANQSVRLVT AAHNHLETVE MVFIATVTGS LSLVTVVGNI LVMLSIKVNR
QLQTVNNYFL FSLGCADLII GAFSMNLYTL YIIKGYWPLG AVVCDLWLAL DYVVSNASVM
NLLIISFDRY FCVTKPLTYP ARRTTKMAGL MIAAAWVLSF VLWAPAILFW QFVVGKRTVP
DNQCFIQFLS NPAVTFGTAI AAFYLPVVIM TVLYIHISLA SRSRVHKHRP EGPKEKKAKT
LAFLKSPLMK PSIKKPPPGG ASREELRNGK LEEAPPPALP PPPRPVPDKD TSNESSSGSA
TQNTKERPPT ELSTAEATTP ALPAPTLQPR TLNPASKWSK IQIVTKQTGN ECVTAIEIVP
ATPAGMRPAA NVARKFASIA RNQVRKKRQM AARERKVTRT IFAILLAFIL TWTPYNVMVL
VNTFCQSCIP ERVWSIGYWL CYVNSTINPA CYALCNATFK KTFRHLLLCQ YRNIGTAR
