ACM5_HUMAN
ID ACM5_HUMAN Reviewed; 532 AA.
AC P08912; Q96RG7;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 2.
DT 03-AUG-2022, entry version 202.
DE RecName: Full=Muscarinic acetylcholine receptor M5;
GN Name=CHRM5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3272174; DOI=10.1016/0896-6273(88)90190-0;
RA Bonner T.I., Young A.C., Brann M.R., Buckley N.J.;
RT "Cloning and expression of the human and rat m5 muscarinic acetylcholine
RT receptor genes.";
RL Neuron 1:403-410(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lens epithelium;
RA Rae J.L., Shepard A.R.;
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RA Wang H., Yeomans J.;
RT "Human muscarinic acetylcholine receptor 5 is localized on 15q13.1 and its
RT expression in SH-SY5Y cells.";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RA Puhl H.L. III, Ikeda S.R., Aronstam R.S.;
RT "cDNA clones of human proteins involved in signal transduction sequenced by
RT the Guthrie cDNA resource center (www.cdna.org).";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-526.
RC TISSUE=Keratinocyte;
RA Arredondo J., Grando S.A.;
RT "Cloning cholinergic receptors in human keratinocytes.";
RL Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: The muscarinic acetylcholine receptor mediates various
CC cellular responses, including inhibition of adenylate cyclase,
CC breakdown of phosphoinositides and modulation of potassium channels
CC through the action of G proteins. Primary transducing effect is Pi
CC turnover.
CC -!- INTERACTION:
CC P08912; P20309: CHRM3; NbExp=7; IntAct=EBI-6655256, EBI-2687785;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Postsynaptic cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; M80333; AAA51569.1; -; Genomic_DNA.
DR EMBL; AF026263; AAB95158.1; -; mRNA.
DR EMBL; AB084282; BAB91222.1; -; mRNA.
DR EMBL; AF498919; AAM18942.1; -; mRNA.
DR EMBL; AF385591; AAK68116.1; -; mRNA.
DR CCDS; CCDS10031.1; -.
DR PIR; JT0530; JT0530.
DR RefSeq; NP_001307846.1; NM_001320917.1.
DR RefSeq; NP_036257.1; NM_012125.3.
DR PDB; 6OL9; X-ray; 2.54 A; A=21-224, A=431-532.
DR PDBsum; 6OL9; -.
DR AlphaFoldDB; P08912; -.
DR SMR; P08912; -.
DR BioGRID; 107555; 36.
DR IntAct; P08912; 30.
DR MINT; P08912; -.
DR STRING; 9606.ENSP00000372750; -.
DR BindingDB; P08912; -.
DR ChEMBL; CHEMBL2035; -.
DR DrugBank; DB08897; Aclidinium.
DR DrugBank; DB05752; ALKS 27.
DR DrugBank; DB00321; Amitriptyline.
DR DrugBank; DB00543; Amoxapine.
DR DrugBank; DB01238; Aripiprazole.
DR DrugBank; DB14185; Aripiprazole lauroxil.
DR DrugBank; DB00572; Atropine.
DR DrugBank; DB00767; Benzquinamide.
DR DrugBank; DB01019; Bethanechol.
DR DrugBank; DB00835; Brompheniramine.
DR DrugBank; DB01239; Chlorprothixene.
DR DrugBank; DB00568; Cinnarizine.
DR DrugBank; DB00363; Clozapine.
DR DrugBank; DB00496; Darifenacin.
DR DrugBank; DB01151; Desipramine.
DR DrugBank; DB09167; Dosulepin.
DR DrugBank; DB01142; Doxepin.
DR DrugBank; DB00366; Doxylamine.
DR DrugBank; DB09194; Etoperidone.
DR DrugBank; DB06702; Fesoterodine.
DR DrugBank; DB00483; Gallamine triethiodide.
DR DrugBank; DB00986; Glycopyrronium.
DR DrugBank; DB11181; Homatropine.
DR DrugBank; DB00725; Homatropine methylbromide.
DR DrugBank; DB00458; Imipramine.
DR DrugBank; DB01221; Ketamine.
DR DrugBank; DB00408; Loxapine.
DR DrugBank; DB00934; Maprotiline.
DR DrugBank; DB00454; Meperidine.
DR DrugBank; DB06709; Methacholine.
DR DrugBank; DB00940; Methantheline.
DR DrugBank; DB01403; Methotrimeprazine.
DR DrugBank; DB00340; Metixene.
DR DrugBank; DB00622; Nicardipine.
DR DrugBank; DB00540; Nortriptyline.
DR DrugBank; DB00715; Paroxetine.
DR DrugBank; DB01069; Promethazine.
DR DrugBank; DB00777; Propiomazine.
DR DrugBank; DB12278; Propiverine.
DR DrugBank; DB01224; Quetiapine.
DR DrugBank; DB11855; Revefenacin.
DR DrugBank; DB13581; Rociverine.
DR DrugBank; DB00747; Scopolamine.
DR DrugBank; DB01591; Solifenacin.
DR DrugBank; DB00342; Terfenadine.
DR DrugBank; DB11235; Thonzylamine.
DR DrugBank; DB01409; Tiotropium.
DR DrugBank; DB01036; Tolterodine.
DR DrugBank; DB00376; Trihexyphenidyl.
DR DrugBank; DB00726; Trimipramine.
DR DrugBank; DB09076; Umeclidinium.
DR DrugBank; DB00246; Ziprasidone.
DR DrugCentral; P08912; -.
DR GuidetoPHARMACOLOGY; 17; -.
DR GlyGen; P08912; 1 site.
DR iPTMnet; P08912; -.
DR PhosphoSitePlus; P08912; -.
DR BioMuta; CHRM5; -.
DR DMDM; 543761; -.
DR PaxDb; P08912; -.
DR PeptideAtlas; P08912; -.
DR PRIDE; P08912; -.
DR Antibodypedia; 2963; 253 antibodies from 28 providers.
DR DNASU; 1133; -.
DR Ensembl; ENST00000383263.7; ENSP00000372750.5; ENSG00000184984.10.
DR Ensembl; ENST00000557872.1; ENSP00000453745.1; ENSG00000184984.10.
DR GeneID; 1133; -.
DR KEGG; hsa:1133; -.
DR MANE-Select; ENST00000383263.7; ENSP00000372750.5; NM_012125.4; NP_036257.1.
DR UCSC; uc001zhk.2; human.
DR CTD; 1133; -.
DR DisGeNET; 1133; -.
DR GeneCards; CHRM5; -.
DR HGNC; HGNC:1954; CHRM5.
DR HPA; ENSG00000184984; Tissue enriched (brain).
DR MIM; 118496; gene.
DR neXtProt; NX_P08912; -.
DR OpenTargets; ENSG00000184984; -.
DR PharmGKB; PA26486; -.
DR VEuPathDB; HostDB:ENSG00000184984; -.
DR eggNOG; KOG4220; Eukaryota.
DR GeneTree; ENSGT00940000158450; -.
DR HOGENOM; CLU_009579_11_2_1; -.
DR InParanoid; P08912; -.
DR OMA; RHGLWEV; -.
DR OrthoDB; 1245472at2759; -.
DR PhylomeDB; P08912; -.
DR TreeFam; TF320495; -.
DR PathwayCommons; P08912; -.
DR Reactome; R-HSA-390648; Muscarinic acetylcholine receptors.
DR Reactome; R-HSA-416476; G alpha (q) signalling events.
DR SignaLink; P08912; -.
DR SIGNOR; P08912; -.
DR BioGRID-ORCS; 1133; 12 hits in 1073 CRISPR screens.
DR GeneWiki; Muscarinic_acetylcholine_receptor_M5; -.
DR GenomeRNAi; 1133; -.
DR Pharos; P08912; Tclin.
DR PRO; PR:P08912; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; P08912; protein.
DR Bgee; ENSG00000184984; Expressed in corpus callosum and 79 other tissues.
DR ExpressionAtlas; P08912; baseline and differential.
DR Genevisible; P08912; HS.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0016907; F:G protein-coupled acetylcholine receptor activity; IBA:GO_Central.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0004435; F:phosphatidylinositol phospholipase C activity; TAS:ProtInc.
DR GO; GO:0007197; P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0015872; P:dopamine transport; IEA:Ensembl.
DR GO; GO:0007213; P:G protein-coupled acetylcholine receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0001696; P:gastric acid secretion; IEA:InterPro.
DR GO; GO:0060304; P:regulation of phosphatidylinositol dephosphorylation; IEA:Ensembl.
DR GO; GO:0019226; P:transmission of nerve impulse; IEA:Ensembl.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000502; Musac_Ach_M5_rcpt.
DR InterPro; IPR000995; Musac_Ach_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00243; MUSCARINICR.
DR PRINTS; PR00542; MUSCRINICM5R.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Membrane; Phosphoprotein; Postsynaptic cell membrane;
KW Receptor; Reference proteome; Synapse; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..532
FT /note="Muscarinic acetylcholine receptor M5"
FT /id="PRO_0000069042"
FT TOPO_DOM 1..29
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 30..53
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 54..66
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 67..87
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 88..104
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 105..126
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 127..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 147..169
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 170..191
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 192..214
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 215..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 444..464
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 465..478
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 479..498
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 499..532
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 262..294
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 263..294
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 501
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 505
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 103..183
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 526
FT /note="Q -> P (in Ref. 5)"
FT /evidence="ECO:0000305"
FT HELIX 27..57
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 59..61
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 64..81
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 83..93
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 100..133
FT /evidence="ECO:0007829|PDB:6OL9"
FT TURN 135..138
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 139..141
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 144..173
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 187..189
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 191..201
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 203..218
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 431..466
FT /evidence="ECO:0007829|PDB:6OL9"
FT STRAND 468..470
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 474..498
FT /evidence="ECO:0007829|PDB:6OL9"
FT HELIX 500..511
FT /evidence="ECO:0007829|PDB:6OL9"
SQ SEQUENCE 532 AA; 60074 MW; EE846186F645DD9B CRC64;
MEGDSYHNAT TVNGTPVNHQ PLERHRLWEV ITIAAVTAVV SLITIVGNVL VMISFKVNSQ
LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWALGS LACDLWLALD YVASNASVMN
LLVISFDRYF SITRPLTYRA KRTPKRAGIM IGLAWLISFI LWAPAILCWQ YLVGKRTVPL
DECQIQFLSE PTITFGTAIA AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVTKAE
KRKPAHRALF RSCLRCPRPT LAQRERNQAS WSSSRRSTST TGKPSQATGP SANWAKAEQL
TTCSSYPSSE DEDKPATDPV LQVVYKSQGK ESPGEEFSAE ETEETFVKAE TEKSDYDTPN
YLLSPAAAHR PKSQKCVAYK FRLVVKADGN QETNNGCHKV KIMPCPFPVA KEPSTKGLNP
NPSHQMTKRK RVVLVKERKA AQTLSAILLA FIITWTPYNI MVLVSTFCDK CVPVTLWHLG
YWLCYVNSTV NPICYALCNR TFRKTFKMLL LCRWKKKKVE EKLYWQGNSK LP
