ACM5_MOUSE
ID ACM5_MOUSE Reviewed; 532 AA.
AC Q920H4; A2AHU3; Q0VBI9;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Muscarinic acetylcholine receptor M5;
GN Name=Chrm5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Yamada M., Wess J.;
RT "Isolation, sequence and functional expression of the mouse M1-M5
RT muscarinic acetylcholine receptor genes.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: The muscarinic acetylcholine receptor mediates various
CC cellular responses, including inhibition of adenylate cyclase,
CC breakdown of phosphoinositides and modulation of potassium channels
CC through the action of G proteins. Primary transducing effect is Pi
CC turnover (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC Postsynaptic cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC Muscarinic acetylcholine receptor subfamily. CHRM5 sub-subfamily.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; AF264051; AAL26028.1; -; Genomic_DNA.
DR EMBL; BC120615; AAI20616.1; -; mRNA.
DR EMBL; AL731840; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS16556.1; -.
DR RefSeq; NP_991352.2; NM_205783.2.
DR RefSeq; XP_006499162.1; XM_006499099.3.
DR AlphaFoldDB; Q920H4; -.
DR SMR; Q920H4; -.
DR IntAct; Q920H4; 1.
DR STRING; 10090.ENSMUSP00000097185; -.
DR BindingDB; Q920H4; -.
DR ChEMBL; CHEMBL2097162; -.
DR DrugCentral; Q920H4; -.
DR GlyGen; Q920H4; 1 site.
DR iPTMnet; Q920H4; -.
DR PhosphoSitePlus; Q920H4; -.
DR PaxDb; Q920H4; -.
DR PRIDE; Q920H4; -.
DR ProteomicsDB; 285587; -.
DR Antibodypedia; 2963; 253 antibodies from 28 providers.
DR DNASU; 213788; -.
DR Ensembl; ENSMUST00000099589; ENSMUSP00000097185; ENSMUSG00000074939.
DR GeneID; 213788; -.
DR KEGG; mmu:213788; -.
DR UCSC; uc008lpd.2; mouse.
DR CTD; 1133; -.
DR MGI; MGI:109248; Chrm5.
DR VEuPathDB; HostDB:ENSMUSG00000074939; -.
DR eggNOG; KOG4220; Eukaryota.
DR GeneTree; ENSGT00940000158450; -.
DR HOGENOM; CLU_009579_11_2_1; -.
DR InParanoid; Q920H4; -.
DR OMA; RHGLWEV; -.
DR OrthoDB; 1245472at2759; -.
DR PhylomeDB; Q920H4; -.
DR TreeFam; TF320495; -.
DR Reactome; R-MMU-390648; Muscarinic acetylcholine receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR BioGRID-ORCS; 213788; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Chrm5; mouse.
DR PRO; PR:Q920H4; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q920H4; protein.
DR Bgee; ENSMUSG00000074939; Expressed in ventromedial nucleus of hypothalamus and 41 other tissues.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0016934; F:extracellularly glycine-gated chloride channel activity; TAS:MGI.
DR GO; GO:0016907; F:G protein-coupled acetylcholine receptor activity; ISO:MGI.
DR GO; GO:0004993; F:G protein-coupled serotonin receptor activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:0007197; P:adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:0015872; P:dopamine transport; IDA:MGI.
DR GO; GO:0007187; P:G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger; IBA:GO_Central.
DR GO; GO:0001696; P:gastric acid secretion; IEA:InterPro.
DR GO; GO:0060304; P:regulation of phosphatidylinositol dephosphorylation; ISO:MGI.
DR GO; GO:0019226; P:transmission of nerve impulse; IDA:MGI.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR000502; Musac_Ach_M5_rcpt.
DR InterPro; IPR000995; Musac_Ach_rcpt.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR00243; MUSCARINICR.
DR PRINTS; PR00542; MUSCRINICM5R.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Postsynaptic cell membrane; Receptor;
KW Reference proteome; Synapse; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..532
FT /note="Muscarinic acetylcholine receptor M5"
FT /id="PRO_0000069044"
FT TOPO_DOM 1..29
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 30..53
FT /note="Helical; Name=1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 54..66
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 67..87
FT /note="Helical; Name=2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 88..104
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 105..126
FT /note="Helical; Name=3"
FT /evidence="ECO:0000250"
FT TOPO_DOM 127..146
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 147..169
FT /note="Helical; Name=4"
FT /evidence="ECO:0000250"
FT TOPO_DOM 170..191
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 192..214
FT /note="Helical; Name=5"
FT /evidence="ECO:0000250"
FT TOPO_DOM 215..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 444..464
FT /note="Helical; Name=6"
FT /evidence="ECO:0000250"
FT TOPO_DOM 465..478
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 479..498
FT /note="Helical; Name=7"
FT /evidence="ECO:0000250"
FT TOPO_DOM 499..532
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 265..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 265..288
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 501
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT MOD_RES 505
FT /note="Phosphothreonine"
FT /evidence="ECO:0000255"
FT CARBOHYD 8
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 103..183
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 265
FT /note="V -> E (in Ref. 1; AAL26028 and 3; AAI20616)"
FT /evidence="ECO:0000305"
FT CONFLICT 325
FT /note="C -> Y (in Ref. 1; AAL26028 and 3; AAI20616)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 532 AA; 60253 MW; 02AA5498F44C1DA6 CRC64;
MEGESYHNET TVNGTPVNHQ ALERHGLWEV ITIAAVTAVV SLMTIVGNVL VMISFKVNSQ
LKTVNNYYLL SLACADLIIG IFSMNLYTTY ILMGRWVLGS LACDLWLALD YVASNASVMN
LLVISFDRYF SITRPLTYRA KRTPKRAGIM IGLAWLVSFI LWAPAILCWQ YLVGKRTVPP
DECQIQFLSE PTITFGTAIA AFYIPVSVMT ILYCRIYRET EKRTKDLADL QGSDSVAEVK
KRKPAHRTLL RSFFSCPRPS LAQRVRNQAS WSSSRRSTST TGKPTQATDL SADWEKAEQV
TNCSSCPSSE DEAKATTDPV FQVVCKNEAK ESPGKEFNTQ ETKETFVSPR TENNDYDTPK
YFLSPGAAHR LKSQKCVAYK FRLVVKADGT QETNNGCRKV KIMPCSFPVS KDPSTKGLDP
HLSHQMTKRK RMVLVKERKA AQTLSAILLA FIITWTPYNI MVLVSTFCDK CVPVTLWHLG
YWLCYVNSTI NPICYALCNR TFRKTFKLLL LCRWKKKKVE EKLYWQGNSK LP
