COAD_YEAST
ID COAD_YEAST Reviewed; 305 AA.
AC P53332; D6VV54;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Phosphopantetheine adenylyltransferase;
DE Short=PPAT;
DE EC=2.7.7.3;
DE AltName: Full=Coenzyme A biosynthesis protein 4;
DE AltName: Full=Dephospho-CoA pyrophosphorylase;
DE AltName: Full=Pantetheine-phosphate adenylyltransferase;
GN Name=CAB4; OrderedLocusNames=YGR277C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=9090054;
RX DOI=10.1002/(sici)1097-0061(19970315)13:3<251::aid-yea63>3.0.co;2-r;
RA Volckaert G., Voet M., Robben J.;
RT "Sequence analysis of a near-subtelomeric 35.4 kb DNA segment on the right
RT arm of chromosome VII from Saccharomyces cerevisiae carrying the MAL1 locus
RT reveals 15 complete open reading frames, including ZUO1, BGL2 and BIO2
RT genes and an ABC transporter gene.";
RL Yeast 13:251-259(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 94-305.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9133740;
RX DOI=10.1002/(sici)1097-0061(19970330)13:4<365::aid-yea78>3.0.co;2-f;
RA Ruzzi M., Marconi A., Saliola M., Fabiani L., Montebove F., Frontali L.;
RT "The sequence of a 8 kb segment on the right arm of yeast chromosome VII
RT identifies four new open reading frames and the genes for yTAFII145.";
RL Yeast 13:365-368(1997).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP FUNCTION.
RX PubMed=19266201; DOI=10.1007/s00294-009-0234-1;
RA Olzhausen J., Schuebbe S., Schueller H.-J.;
RT "Genetic analysis of coenzyme A biosynthesis in the yeast Saccharomyces
RT cerevisiae: identification of a conditional mutation in the pantothenate
RT kinase gene CAB1.";
RL Curr. Genet. 55:163-173(2009).
CC -!- FUNCTION: Reversibly transfers an adenylyl group from ATP to 4'-
CC phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate
CC (By similarity). Plays a role in the physiological regulation of the
CC intracellular CoA concentration. {ECO:0000250,
CC ECO:0000269|PubMed:19266201}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA +
CC diphosphate; Xref=Rhea:RHEA:19801, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57328,
CC ChEBI:CHEBI:61723; EC=2.7.7.3;
CC -!- INTERACTION:
CC P53332; P40506: CAB2; NbExp=6; IntAct=EBI-23648, EBI-25089;
CC P53332; P36076: CAB3; NbExp=5; IntAct=EBI-23648, EBI-26778;
CC P53332; Q03941: CAB5; NbExp=6; IntAct=EBI-23648, EBI-22174;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14690591}. Nucleus
CC {ECO:0000269|PubMed:14690591}.
CC -!- MISCELLANEOUS: Present with 3610 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the eukaryotic CoaD family. {ECO:0000305}.
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DR EMBL; Z73062; CAA97307.1; -; Genomic_DNA.
DR EMBL; AY692609; AAT92628.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA08365.1; -; Genomic_DNA.
DR PIR; S64612; S64612.
DR RefSeq; NP_011793.3; NM_001181406.3.
DR AlphaFoldDB; P53332; -.
DR SMR; P53332; -.
DR BioGRID; 33527; 255.
DR ComplexPortal; CPX-396; Coenzyme A-synthesizing protein complex.
DR DIP; DIP-4904N; -.
DR IntAct; P53332; 6.
DR MINT; P53332; -.
DR STRING; 4932.YGR277C; -.
DR iPTMnet; P53332; -.
DR MaxQB; P53332; -.
DR PaxDb; P53332; -.
DR PRIDE; P53332; -.
DR EnsemblFungi; YGR277C_mRNA; YGR277C; YGR277C.
DR GeneID; 853194; -.
DR KEGG; sce:YGR277C; -.
DR SGD; S000003509; CAB4.
DR VEuPathDB; FungiDB:YGR277C; -.
DR eggNOG; KOG3351; Eukaryota.
DR HOGENOM; CLU_035272_0_1_1; -.
DR InParanoid; P53332; -.
DR OMA; KDVCGPT; -.
DR BioCyc; MetaCyc:MON3O-487; -.
DR BioCyc; YEAST:MON3O-487; -.
DR Reactome; R-SCE-196783; Coenzyme A biosynthesis.
DR PRO; PR:P53332; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53332; protein.
DR GO; GO:1990143; C:CoA-synthesizing protein complex; IDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004140; F:dephospho-CoA kinase activity; IBA:GO_Central.
DR GO; GO:0004595; F:pantetheine-phosphate adenylyltransferase activity; ISS:SGD.
DR GO; GO:0015937; P:coenzyme A biosynthetic process; IGI:SGD.
DR Gene3D; 3.40.50.620; -; 1.
DR InterPro; IPR004821; Cyt_trans-like.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR Pfam; PF01467; CTP_transf_like; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Coenzyme A biosynthesis; Cytoplasm; Nucleotide-binding;
KW Nucleotidyltransferase; Nucleus; Reference proteome; Transferase.
FT CHAIN 1..305
FT /note="Phosphopantetheine adenylyltransferase"
FT /id="PRO_0000202870"
SQ SEQUENCE 305 AA; 34307 MW; BEB43BEFC54D123C CRC64;
MVEENSRVLI VLPYTPPSAT LQRIIGQTIP FLRECQSQLD IVIVPEFKTS FQLDSALGKM
YSITRDVLLG YGMINSGINI IFNNIHFVES NLQWKVVLLP QESTFETWKL ELGQGQYHSI
EHYALHDNIM EEIEGPKDAN KFHVTALGGT FDHIHDGHKI LLSVSTFITS QRLICGITCD
ELLQNKKYKE LIEPYDTRCR HVHQFIKLLK PDLSVELVPL RDVCGPTGKV PEIECLVVSR
ETVSGAETVN KTRIEKGMSP LAVHVVNVLG GREEDGWSEK LSSTEIRRLL KSSASPTCTP
QNPCV
