ID   ACNR_MYCTU              Reviewed;         187 AA.
AC   O53165; F2GES5; I6Y6M9; Q7D8D9;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 151.
DE   RecName: Full=HTH-type transcriptional repressor Rv1474c {ECO:0000305};
GN   OrderedLocusNames=Rv1474c {ECO:0000312|EMBL:CCP44234.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, ACTIVITY REGULATION, SUBUNIT, INDUCTION, AND MUTAGENESIS OF
RP   ARG-45; HIS-50 AND LYS-55.
RX   PubMed=28237036; DOI=10.1016/j.tube.2017.01.003;
RA   Balakrishnan K., Mohareer K., Banerjee S.;
RT   "Mycobacterium tuberculosis Rv1474c is a TetR-like transcriptional
RT   repressor that regulates aconitase, an essential enzyme and RNA-binding
RT   protein, in an iron-responsive manner.";
RL   Tuberculosis 103:71-82(2017).
CC   -!- FUNCTION: Represses the expression of the aconitase gene acn and its
CC       own expression, in an iron-responsive manner. Binds to the inverted
CC       repeat element present in the upstream region of acn (Rv1475c)-Rv1474c
CC       operon. Preferentially binds to major groove of the DNA.
CC       {ECO:0000269|PubMed:28237036}.
CC   -!- ACTIVITY REGULATION: Binding to DNA is abolished in the presence of
CC       high concentration of iron. Specifically binds to tetracycline, which
CC       leads to a conformational change in the structure of the protein and
CC       inhibits the DNA binding activity. {ECO:0000269|PubMed:28237036}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:28237036}.
CC   -!- INDUCTION: Negatively autoregulated. {ECO:0000269|PubMed:28237036}.
CC   -!- MISCELLANEOUS: Its association with indispensable iron homeostasis
CC       makes Rv1474c an attractive target for designing novel anti-
CC       mycobacterials. {ECO:0000269|PubMed:28237036}.
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DR   EMBL; AL123456; CCP44234.1; -; Genomic_DNA.
DR   RefSeq; NP_215990.1; NC_000962.3.
DR   RefSeq; WP_003407514.1; NZ_NVQJ01000004.1.
DR   AlphaFoldDB; O53165; -.
DR   SMR; O53165; -.
DR   STRING; 83332.Rv1474c; -.
DR   PaxDb; O53165; -.
DR   PRIDE; O53165; -.
DR   GeneID; 886543; -.
DR   KEGG; mtu:Rv1474c; -.
DR   PATRIC; fig|83332.111.peg.1641; -.
DR   TubercuList; Rv1474c; -.
DR   eggNOG; COG1309; Bacteria.
DR   OMA; PEFSRGW; -.
DR   PhylomeDB; O53165; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..187
FT                   /note="HTH-type transcriptional repressor Rv1474c"
FT                   /id="PRO_0000447327"
FT   DOMAIN          10..70
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        33..52
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   MUTAGEN         45
FT                   /note="R->A: Cannot bind DNA; when associated with A-50 and
FT                   A-55."
FT                   /evidence="ECO:0000269|PubMed:28237036"
FT   MUTAGEN         50
FT                   /note="H->A: Cannot bind DNA; when associated with A-45 and
FT                   A-55."
FT                   /evidence="ECO:0000269|PubMed:28237036"
FT   MUTAGEN         55
FT                   /note="K->A: Cannot bind DNA; when associated with A-45 and
FT                   A-50."
FT                   /evidence="ECO:0000269|PubMed:28237036"
SQ   SEQUENCE   187 AA;  21269 MW;  B0FEE0360661172F CRC64;
     MPKVSEDHLA ARRRQILDGA RRCFAEYGYD KATVRRLEQA IGMSRGAIFH HFRDKDALFF
     ALAREDTERM AAVASREGLI GVMRDMLAAP DQFDWLATRL EIARKLRNDP DFSRGWAERS
     AELAAATTDR LRRQKQANRV RDDVPSDVLR CYLDLVLDGL LARLASGEDP QRLAAVLDLV
     ENSVRRS