ID   ACOA_CUPNH              Reviewed;         333 AA.
AC   P27745; Q0K4X4;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha;
DE            Short=Acetoin:DCPIP oxidoreductase-alpha;
DE            Short=Ao:DCPIP OR;
DE            EC=1.1.1.-;
GN   Name=acoA; OrderedLocusNames=H16_B0144;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-32.
RX   PubMed=2061286; DOI=10.1128/jb.173.13.4056-4071.1991;
RA   Priefert H., Hein S., Krueger N., Zeh K., Schmidt B., Steinbuechel A.;
RT   "Identification and molecular characterization of the Alcaligenes eutrophus
RT   H16 aco operon genes involved in acetoin catabolism.";
RL   J. Bacteriol. 173:4056-4071(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- FUNCTION: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage
CC       of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit
CC       is probably the catalytic subunit of the enzyme.
CC   -!- COFACTOR:
CC       Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937;
CC   -!- PATHWAY: Ketone degradation; acetoin degradation.
CC   -!- SUBUNIT: Tetramer of 2 alpha and 2 beta subunits.
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DR   EMBL; M66060; AAA21948.1; -; Genomic_DNA.
DR   EMBL; AM260480; CAJ94950.1; -; Genomic_DNA.
DR   PIR; B42462; DEALXE.
DR   RefSeq; WP_011616379.1; NZ_CP039288.1.
DR   AlphaFoldDB; P27745; -.
DR   SMR; P27745; -.
DR   STRING; 381666.H16_B0144; -.
DR   EnsemblBacteria; CAJ94950; CAJ94950; H16_B0144.
DR   GeneID; 57646023; -.
DR   KEGG; reh:H16_B0144; -.
DR   eggNOG; COG1071; Bacteria.
DR   HOGENOM; CLU_029393_5_0_4; -.
DR   OMA; FGMPGVT; -.
DR   OrthoDB; 1248201at2; -.
DR   BRENDA; 2.3.1.190; 11493.
DR   UniPathway; UPA00040; -.
DR   Proteomes; UP000008210; Chromosome 2.
DR   GO; GO:0016624; F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor; IEA:InterPro.
DR   GO; GO:0045150; P:acetoin catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR001017; DH_E1.
DR   InterPro; IPR029061; THDP-binding.
DR   Pfam; PF00676; E1_dh; 1.
DR   SUPFAM; SSF52518; SSF52518; 1.
PE   1: Evidence at protein level;
KW   Acetoin catabolism; Direct protein sequencing; Oxidoreductase;
KW   Reference proteome; Thiamine pyrophosphate.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2061286"
FT   CHAIN           2..333
FT                   /note="Acetoin:2,6-dichlorophenolindophenol oxidoreductase
FT                   subunit alpha"
FT                   /id="PRO_0000162307"
SQ   SEQUENCE   333 AA;  35375 MW;  06156073C9BC869F CRC64;
     MTARASQDSA ALPLDKETLL TVYRKMRTIR DFEERLHVDF GRGDIPGFVH LYAGEEAAGV
     GILHHLNDGD RIASTHRGHG HCIAKGVDPV AMMKEIYGKK GGSCNGKGGS MHIADLSKGM
     MGANGILGAG APLICGAALA AKFRGKGEVG ITFCGDGASN QGTFLESLNL AAVWNLPVIF
     VIENNGYAES TSRDYGTAVD SYVDRAAGFG IPGVTVDGTD FFAVHEAAGE VIRRAREGGG
     PSLLECKMVR FYGHFEGDAQ TYRAAGELDD IRANKDCLKL FGRAVTQAGV VAREELDTID
     REVAALIEHA VQEAKAAPQP GPEDLLTDVY VSY