ACOHC_DICDI
ID ACOHC_DICDI Reviewed; 894 AA.
AC Q54X73;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Probable cytoplasmic aconitate hydratase {ECO:0000305};
DE Short=Aconitase;
DE EC=4.2.1.3 {ECO:0000250|UniProtKB:P21399};
DE AltName: Full=Citrate hydro-lyase;
GN Name=aco1; Synonyms=acnA; ORFNames=DDB_G0279159;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000250|UniProtKB:P21399}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000250|UniProtKB:P21399};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000250|UniProtKB:P21399};
CC Note=Binds 1 [4Fe-4S] cluster per subunit.
CC {ECO:0000250|UniProtKB:P21399};
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:P21399}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000028; EAL67861.1; -; Genomic_DNA.
DR RefSeq; XP_641837.1; XM_636745.1.
DR AlphaFoldDB; Q54X73; -.
DR SMR; Q54X73; -.
DR STRING; 44689.DDB0229908; -.
DR PaxDb; Q54X73; -.
DR EnsemblProtists; EAL67861; EAL67861; DDB_G0279159.
DR GeneID; 8621900; -.
DR KEGG; ddi:DDB_G0279159; -.
DR dictyBase; DDB_G0279159; aco1.
DR eggNOG; KOG0452; Eukaryota.
DR HOGENOM; CLU_013476_2_1_1; -.
DR InParanoid; Q54X73; -.
DR OMA; NGGIMQY; -.
DR PhylomeDB; Q54X73; -.
DR Reactome; R-DDI-917937; Iron uptake and transport.
DR PRO; PR:Q54X73; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0003994; F:aconitate hydratase activity; IBA:GO_Central.
DR GO; GO:0047780; F:citrate dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0030350; F:iron-responsive element binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006101; P:citrate metabolic process; IBA:GO_Central.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 3.20.19.10; -; 1.
DR Gene3D; 3.30.499.10; -; 2.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR PANTHER; PTHR11670; PTHR11670; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; SSF53732; 1.
DR TIGRFAMs; TIGR01341; aconitase_1; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Cytoplasm; Iron; Iron-sulfur; Lyase; Metal-binding;
KW Reference proteome; Tricarboxylic acid cycle.
FT CHAIN 1..894
FT /note="Probable cytoplasmic aconitate hydratase"
FT /id="PRO_0000328573"
FT BINDING 87
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 207..209
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 438
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 504
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 507
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 537
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 542
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 781..782
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 894 AA; 97991 MW; 8CE9854114B55202 CRC64;
MTTNNPFDKV KDVLKSQDQT YNFYNLSKLQ DPRIEKLPYS IRILLESAVR NCDNFEVHEK
DVENILNWEN TANKVEIPFK PARVLLQDFT GVPAVVDLAA MRDAMKRLGG DPAKINPLVP
VDLVIDHSVQ VDVSRTVDAL EQNQKIEFHR NHERFSFLKW GAQAFDGLFI APPGSGIVHQ
VNLEYIAREV MNGTGNLLYP DSVVGTDSHT TMINGLGVCG WGVGGIEAEA VMLGQPMSMV
LPEVVGYKFV GKLPDIATAT DLVLTVTNEL RKKGVVGKFV EFYGEGVSTL SVQDRATISN
MAPEYGATMG FFPADENTID YLASTGRSNT KIEYIKNYLS SQGLMCNYKS QSHPIFTTTM
ELDLSTVVPS LSGPKRPHDR ISLNSMKQDF NSCLSSPVGF KGFGLTADQI QKKATFTFKD
KQYTIGHGAV TIAAITSCTN TSNPSVMLGA GLLAKNAVEH GLEVAPYIKT SLSPGSGVVT
EYFSHSGLQE PLNKLGFDLT GYGCMTCIGN SGELAEPLAE AITKEDLVVA GVLSGNRNFE
GRIHPLLRAN YLASPPLVVA YALAGTVDID FETTPLGVSK KTGQPVFLRD IWPSKDLIQQ
TIKSSVLPDM YERVYSNVND GNKSWNELKV PTGLLYPWDE KSTYIHNPPF FKTMELTVSK
RPAITNAYCL LNLGDSITTD HISPAGNINR KSSAARYLES KGVKPEDFNT YGSRRGNDEI
MVRGTFANTR IVNKLAPAVG PQTTYVPTGE LMFISDAAEK YQSEGHQLIV LAGSDYGSGS
SRDWAAKGPY LQGIKCVIAI SFERIHRSNL VGMGIIPLQF QPGQNASTLG LTGKEQFNIE
LPTDKSLIKT GQTVKVTTNC GKSFETILRF DTPIEVEYWA NNGILSYVLR KLLH
