ACOHC_ORYSJ
ID ACOHC_ORYSJ Reviewed; 898 AA.
AC Q6YZX6;
DT 25-JUL-2006, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Putative aconitate hydratase, cytoplasmic;
DE Short=Aconitase;
DE EC=4.2.1.3;
DE AltName: Full=Citrate hydro-lyase;
GN OrderedLocusNames=Os08g0191100, LOC_Os08g09200; ORFNames=P0610E02.32;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster per subunit. {ECO:0000250};
CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate and dicarboxylate
CC metabolism.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000305}.
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DR EMBL; AP005505; BAD05751.1; -; Genomic_DNA.
DR EMBL; AP014964; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; Q6YZX6; -.
DR SMR; Q6YZX6; -.
DR STRING; 39947.Q6YZX6; -.
DR CarbonylDB; Q6YZX6; -.
DR PaxDb; Q6YZX6; -.
DR PRIDE; Q6YZX6; -.
DR InParanoid; Q6YZX6; -.
DR PlantReactome; R-OSA-1119533; TCA cycle (plant).
DR PlantReactome; R-OSA-1119540; Leucine biosynthesis.
DR UniPathway; UPA00227; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IBA:GO_Central.
DR GO; GO:0003994; F:aconitate hydratase activity; IBA:GO_Central.
DR GO; GO:0047780; F:citrate dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0030350; F:iron-responsive element binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006101; P:citrate metabolic process; IBA:GO_Central.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IBA:GO_Central.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 3.20.19.10; -; 1.
DR Gene3D; 3.30.499.10; -; 2.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR PANTHER; PTHR11670; PTHR11670; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; SSF53732; 1.
DR TIGRFAMs; TIGR01341; aconitase_1; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Cytoplasm; Glyoxylate bypass; Iron; Iron-sulfur; Lyase;
KW Metal-binding; Reference proteome.
FT CHAIN 1..898
FT /note="Putative aconitate hydratase, cytoplasmic"
FT /id="PRO_0000247306"
FT BINDING 90
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 209..211
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 441
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 507
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 510
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 540
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 545
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 703
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 784..785
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 898 AA; 98083 MW; BB808EB283E695A8 CRC64;
MAAEHPFKNI LTTLPKPGGG EYGKFYSLPA LNDPRIDKLP YSIRILLESA IRNCDNFQVN
QNDVEKIIDW ENTSPKLAEI PFKPARVLLQ DFTGVPAVVD LAAMRDAMAK LGSDANKINP
LVPVDLVIDH SVQVDVARSP NAVQSNMELE FKRNNERFGF LKWGSTAFHN MLVVPPGSGI
VHQVNLEYLG RVVFNTDGIM YPDSVVGTDS HTTMIDGLGV AGWGVGGIEA EATMLGQPMS
MVLPGVVGFK LTGKLQNGVT ATDLVLTVTQ MLRKHGVVGK FVEFYGEGMG KLSLADRATI
ANMSPEYGAT MGFFPVDHVT LDYLKLTGRS DETVAMIEAY LRANKMFVDY NEPQTERVYS
SYLELDLNEV EPCISGPKRP HDRVLLKEMK SDWHSCLDNR VGFKGFAVPK EQQDKVVKFD
FHGQPAELKH GSVVIAAITS CTNTSNPSVM LGAALVAKKA CELGLEVKPW VKTSLAPGSG
VVTKYLLQSG LQEYLNKQGF HVVGYGCTTC IGNSGDLDES VSAAISENDV VAAAVLSGNR
NFEGRVHPLT RANYLASPPL VVAYALAGTV DIDFEKEPIG VGKDGKEVFF RDIWPSTEEI
AEVVQSSVLP DMFKSTYEAI TKGNPMWNQL TVPEASLYSW DPNSTYIHEP PYFKDMTMSP
PGPHGVKNAY CLLNFGDSIT TDHISPAGSI HKDSPAAKYL LERGVDRKDF NSYGSRRGND
EVMARGTFAN IRIVNKFLNG EVGPKTVHVP TGEKLYVFDA ALKYKSEGHD TIVLAGAEYG
SGSSRDWAAK GPMLLGVKAV IAKSFERIHR SNLVGMGIIP LCFKAGEDAD SLGLTGHERY
TIDLPTNVSE IRPGQDITVT TDNGKSFTCT LRFDTEVELA YFNHGGILPY VIRNLAQN
