COB23_ARATH
ID COB23_ARATH Reviewed; 909 AA.
AC Q8L828; Q9LW87;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 148.
DE RecName: Full=Coatomer subunit beta'-3;
DE AltName: Full=Beta'-coat protein 3;
DE Short=Beta'-COP 3;
GN OrderedLocusNames=At3g15980; ORFNames=MSL1.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
CC -!- FUNCTION: The coatomer is a cytosolic protein complex that binds to
CC dilysine motifs and reversibly associates with Golgi non-clathrin-
CC coated vesicles, which further mediate biosynthetic protein transport
CC from the ER, via the Golgi up to the trans Golgi network. Coatomer
CC complex is required for budding from Golgi membranes, and is essential
CC for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
CC (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Oligomeric complex that consists of at least the alpha, beta,
CC beta', gamma, delta, epsilon and zeta subunits. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Golgi apparatus membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Cytoplasmic vesicle, COPI-coated vesicle membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=The coatomer is cytoplasmic or polymerized on
CC the cytoplasmic side of the Golgi, as well as on the vesicles/buds
CC originating from it. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8L828-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8L828-2; Sequence=VSP_024872;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the WD repeat COPB2 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02664.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB012247; BAB02664.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75755.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75756.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75757.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75758.2; -; Genomic_DNA.
DR EMBL; CP002686; ANM65912.1; -; Genomic_DNA.
DR EMBL; AY120786; AAM53344.1; -; mRNA.
DR EMBL; BT001201; AAN65088.1; -; mRNA.
DR RefSeq; NP_001319563.1; NM_001338195.1. [Q8L828-2]
DR RefSeq; NP_001327848.1; NM_001338196.1. [Q8L828-2]
DR RefSeq; NP_188219.2; NM_112468.3. [Q8L828-2]
DR RefSeq; NP_850592.1; NM_180261.2. [Q8L828-1]
DR RefSeq; NP_850593.1; NM_180262.2. [Q8L828-2]
DR AlphaFoldDB; Q8L828; -.
DR SMR; Q8L828; -.
DR BioGRID; 6176; 2.
DR STRING; 3702.AT3G15980.5; -.
DR iPTMnet; Q8L828; -.
DR PaxDb; Q8L828; -.
DR ProteomicsDB; 241137; -. [Q8L828-1]
DR EnsemblPlants; AT3G15980.1; AT3G15980.1; AT3G15980. [Q8L828-1]
DR EnsemblPlants; AT3G15980.2; AT3G15980.2; AT3G15980. [Q8L828-2]
DR EnsemblPlants; AT3G15980.3; AT3G15980.3; AT3G15980. [Q8L828-2]
DR EnsemblPlants; AT3G15980.4; AT3G15980.4; AT3G15980. [Q8L828-2]
DR EnsemblPlants; AT3G15980.6; AT3G15980.6; AT3G15980. [Q8L828-2]
DR GeneID; 820842; -.
DR Gramene; AT3G15980.1; AT3G15980.1; AT3G15980. [Q8L828-1]
DR Gramene; AT3G15980.2; AT3G15980.2; AT3G15980. [Q8L828-2]
DR Gramene; AT3G15980.3; AT3G15980.3; AT3G15980. [Q8L828-2]
DR Gramene; AT3G15980.4; AT3G15980.4; AT3G15980. [Q8L828-2]
DR Gramene; AT3G15980.6; AT3G15980.6; AT3G15980. [Q8L828-2]
DR KEGG; ath:AT3G15980; -.
DR Araport; AT3G15980; -.
DR eggNOG; KOG0276; Eukaryota.
DR HOGENOM; CLU_005507_1_0_1; -.
DR PhylomeDB; Q8L828; -.
DR PRO; PR:Q8L828; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8L828; baseline and differential.
DR Genevisible; Q8L828; AT.
DR GO; GO:0030126; C:COPI vesicle coat; IBA:GO_Central.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR GO; GO:0006888; P:endoplasmic reticulum to Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR006692; Coatomer_WD-assoc_reg.
DR InterPro; IPR016453; COPB2.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF04053; Coatomer_WDAD; 1.
DR Pfam; PF00400; WD40; 4.
DR PIRSF; PIRSF005567; Coatomer_beta'_subunit; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Cytoplasmic vesicle; ER-Golgi transport;
KW Golgi apparatus; Membrane; Protein transport; Reference proteome; Repeat;
KW Transport; WD repeat.
FT CHAIN 1..909
FT /note="Coatomer subunit beta'-3"
FT /id="PRO_0000285606"
FT REPEAT 13..52
FT /note="WD 1"
FT REPEAT 55..94
FT /note="WD 2"
FT REPEAT 97..136
FT /note="WD 3"
FT REPEAT 140..180
FT /note="WD 4"
FT REPEAT 183..224
FT /note="WD 5"
FT REPEAT 227..266
FT /note="WD 6"
FT REPEAT 269..309
FT /note="WD 7"
FT REPEAT 351..390
FT /note="WD 8"
FT REPEAT 461..501
FT /note="WD 9"
FT REGION 862..909
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 866..890
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 904..909
FT /note="VLTPHP -> GEEEWGTNNKGNPSA (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_024872"
SQ SEQUENCE 909 AA; 102476 MW; E4BB1C729901D595 CRC64;
MPLRLDIKRK FAQRSERVKS VDLHPTEPWI LASLYSGTVC IWNYQTQTIT KSFEVTELPV
RSAKFIPRKQ WVVAGADDMY IRVYNYNTMD KVKVFEAHSD YIRCVAVHPT LPYVLSSSDD
MLIKLWDWEN GWACTQIFEG HSHYVMQVVF NPKDTNTFAS ASLDRTIKIW NLGSPDPNFT
LDAHQKGVNC VDYFTGGDKP YLITGSDDHT AKVWDYQTKS CVQTLDGHTH NVSAVCFHPE
LPIIITGSED GTVRIWHATT YRLENTLNYG LERVWAIGYI KSSRRVVIGY DEGTIMVKLG
REIPVASMDS SGKIIWAKHN EIQTANIKSI GAGYEATDGE RLPLSVKELG TCDLYPQSLK
HNPNGRFVVV CGDGEYIIYT ALAWRNRSFG SGLEFVWSSE GECAVRESSS KIKIFSKNFQ
ERKSIRPTFS AEKIFGGTLL AMCSNDFICF YDWAECRLIQ QIDVTVKNLY WAESGDLVAI
ASDTSFYILK YNRELVSSHF DSGRPTDEEG VEDAFEVLHE NDERVRTGIW VGDCFIYNNS
SWKLNYCVGG EVTTMYHLDR PMYLLGYIAN QSRVYLVDKE FNVIGYTLLL SLIEYKTLVM
RGDLDRANQI LPTIPKEQHN NVAHFLESRG MIEDALEIAT DPDYKFDLAI QLGRLEIAKE
IAEEVQSESK WKQLGELAMS SGKLQLAEDC MKYAMDLSGL LLLYSSLGDA EGVSKLACLA
KEQGKNNVAF LCLFTLGRLE DCLQLLVESN RIPEAALMAR SYLPSKVSEI VALWREDLSK
VNPKAAESLA DPEEYSNLFE DWQVALSVEA NTAETRGVYT AAENYPSHAD KPSITLVEAF
RNLQVEAEES LENGNIDHEV AEENGHVENE GDEEEQQEEE VNEEEGVVDA DSTDGAVLVN
GSEVLTPHP
