ID   COBH_MYCBO              Reviewed;         208 AA.
AC   P63840; A0A1R3Y023; Q10676; X2BJM5;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Precorrin-8X methylmutase;
DE            EC=5.4.99.61;
DE   AltName: Full=HBA synthase;
DE   AltName: Full=Precorrin isomerase;
GN   Name=cobH; OrderedLocusNames=BQ2027_MB2091;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Catalyzes the conversion of precorrin-8X to
CC       hydrogenobyrinate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 H(+) + precorrin-8X = hydrogenobyrinate;
CC         Xref=Rhea:RHEA:22512, ChEBI:CHEBI:15378, ChEBI:CHEBI:58581,
CC         ChEBI:CHEBI:77873; EC=5.4.99.61;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 8/10.
CC   -!- SIMILARITY: Belongs to the CobH/CbiC family. {ECO:0000305}.
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DR   EMBL; LT708304; SIU00698.1; -; Genomic_DNA.
DR   RefSeq; NP_855741.1; NC_002945.3.
DR   RefSeq; WP_003410658.1; NC_002945.4.
DR   AlphaFoldDB; P63840; -.
DR   SMR; P63840; -.
DR   EnsemblBacteria; SIU00698; SIU00698; BQ2027_MB2091.
DR   PATRIC; fig|233413.5.peg.2299; -.
DR   OMA; GAPIFCD; -.
DR   UniPathway; UPA00148; UER00219.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0016993; F:precorrin-8X methylmutase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.10230; -; 1.
DR   InterPro; IPR003722; Cbl_synth_CobH/CbiC.
DR   InterPro; IPR036588; CobH/CbiC_sf.
DR   Pfam; PF02570; CbiC; 1.
DR   SUPFAM; SSF63965; SSF63965; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Isomerase.
FT   CHAIN           1..208
FT                   /note="Precorrin-8X methylmutase"
FT                   /id="PRO_0000135925"
FT   ACT_SITE        41
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         15
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         38
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   208 AA;  21614 MW;  975925A6C669D497 CRC64;
     MLDYLRDAAE IYRRSFAVIR AEADLARFPA DVARVVVRLI HTCGQVDVAE HVAYTDDVVA
     RAGAALAAGA PVLCDSSMVA AGITTSRLPA DNQIVSLVAD PRATELAARR QTTRSAAGVE
     LCAERLPGAV LAIGNAPTAL FRLLELVDEG APPPAAVLGG PVGFVGSAQA KEELIERPRG
     MSYLVVRGRR GGSAMAAAAV NAIASDRE