ID   COBH_MYCTO              Reviewed;         208 AA.
AC   P9WP86; L0TBD9; P63839; Q10676;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Precorrin-8X methylmutase;
DE            EC=5.4.99.61;
DE   AltName: Full=HBA synthase;
DE   AltName: Full=Precorrin isomerase;
GN   Name=cobH; OrderedLocusNames=MT2125;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Catalyzes the conversion of precorrin-8X to
CC       hydrogenobyrinate. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=3 H(+) + precorrin-8X = hydrogenobyrinate;
CC         Xref=Rhea:RHEA:22512, ChEBI:CHEBI:15378, ChEBI:CHEBI:58581,
CC         ChEBI:CHEBI:77873; EC=5.4.99.61;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 8/10.
CC   -!- SIMILARITY: Belongs to the CobH/CbiC family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK46405.1; -; Genomic_DNA.
DR   PIR; D70764; D70764.
DR   RefSeq; WP_003410658.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WP86; -.
DR   SMR; P9WP86; -.
DR   EnsemblBacteria; AAK46405; AAK46405; MT2125.
DR   KEGG; mtc:MT2125; -.
DR   PATRIC; fig|83331.31.peg.2293; -.
DR   HOGENOM; CLU_084703_0_0_11; -.
DR   UniPathway; UPA00148; UER00219.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016993; F:precorrin-8X methylmutase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.10230; -; 1.
DR   InterPro; IPR003722; Cbl_synth_CobH/CbiC.
DR   InterPro; IPR036588; CobH/CbiC_sf.
DR   Pfam; PF02570; CbiC; 1.
DR   SUPFAM; SSF63965; SSF63965; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Isomerase.
FT   CHAIN           1..208
FT                   /note="Precorrin-8X methylmutase"
FT                   /id="PRO_0000426995"
FT   ACT_SITE        41
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         15
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         38
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   208 AA;  21614 MW;  975925A6C669D497 CRC64;
     MLDYLRDAAE IYRRSFAVIR AEADLARFPA DVARVVVRLI HTCGQVDVAE HVAYTDDVVA
     RAGAALAAGA PVLCDSSMVA AGITTSRLPA DNQIVSLVAD PRATELAARR QTTRSAAGVE
     LCAERLPGAV LAIGNAPTAL FRLLELVDEG APPPAAVLGG PVGFVGSAQA KEELIERPRG
     MSYLVVRGRR GGSAMAAAAV NAIASDRE