COBH_MYCTU
ID COBH_MYCTU Reviewed; 208 AA.
AC P9WP87; L0TBD9; P63839; Q10676;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 33.
DE RecName: Full=Precorrin-8X methylmutase;
DE EC=5.4.99.61;
DE AltName: Full=HBA synthase;
DE AltName: Full=Precorrin isomerase;
GN Name=cobH; OrderedLocusNames=Rv2065; ORFNames=MTCY49.04;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Catalyzes the conversion of precorrin-8X to
CC hydrogenobyrinate. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3 H(+) + precorrin-8X = hydrogenobyrinate;
CC Xref=Rhea:RHEA:22512, ChEBI:CHEBI:15378, ChEBI:CHEBI:58581,
CC ChEBI:CHEBI:77873; EC=5.4.99.61;
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 8/10.
CC -!- SIMILARITY: Belongs to the CobH/CbiC family. {ECO:0000305}.
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DR EMBL; AL123456; CCP44839.1; -; Genomic_DNA.
DR PIR; D70764; D70764.
DR RefSeq; NP_216581.1; NC_000962.3.
DR RefSeq; WP_003410658.1; NZ_NVQJ01000047.1.
DR AlphaFoldDB; P9WP87; -.
DR SMR; P9WP87; -.
DR STRING; 83332.Rv2065; -.
DR PaxDb; P9WP87; -.
DR DNASU; 888464; -.
DR GeneID; 888464; -.
DR KEGG; mtu:Rv2065; -.
DR TubercuList; Rv2065; -.
DR eggNOG; COG2082; Bacteria.
DR OMA; GAPIFCD; -.
DR PhylomeDB; P9WP87; -.
DR UniPathway; UPA00148; UER00219.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0016993; F:precorrin-8X methylmutase activity; IEA:UniProtKB-EC.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.40.50.10230; -; 1.
DR InterPro; IPR003722; Cbl_synth_CobH/CbiC.
DR InterPro; IPR036588; CobH/CbiC_sf.
DR Pfam; PF02570; CbiC; 1.
DR SUPFAM; SSF63965; SSF63965; 1.
PE 1: Evidence at protein level;
KW Cobalamin biosynthesis; Isomerase; Reference proteome.
FT CHAIN 1..208
FT /note="Precorrin-8X methylmutase"
FT /id="PRO_0000135924"
FT ACT_SITE 41
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250"
FT BINDING 15
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 38
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 208 AA; 21614 MW; 975925A6C669D497 CRC64;
MLDYLRDAAE IYRRSFAVIR AEADLARFPA DVARVVVRLI HTCGQVDVAE HVAYTDDVVA
RAGAALAAGA PVLCDSSMVA AGITTSRLPA DNQIVSLVAD PRATELAARR QTTRSAAGVE
LCAERLPGAV LAIGNAPTAL FRLLELVDEG APPPAAVLGG PVGFVGSAQA KEELIERPRG
MSYLVVRGRR GGSAMAAAAV NAIASDRE