COBIJ_MYCTO
ID COBIJ_MYCTO Reviewed; 508 AA.
AC P9WGB2; L0TB91; P66877; Q10677;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 38.
DE RecName: Full=Cobalamin biosynthesis protein CobIJ;
DE Includes:
DE RecName: Full=Precorrin-2 C(20)-methyltransferase;
DE EC=2.1.1.130;
DE AltName: Full=S-adenosyl-L-methionine--precorrin-2 methyltransferase;
DE Short=SP2MT;
DE Includes:
DE RecName: Full=Precorrin-3B C17-methyltransferase;
DE EC=2.1.1.131;
DE AltName: Full=S-adenosyl-L-methionine--precorrin-3B methyltransferase;
GN Name=cobIJ; Synonyms=cobI; OrderedLocusNames=MT2126;
OS Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83331;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT laboratory strains.";
RL J. Bacteriol. 184:5479-5490(2002).
CC -!- FUNCTION: Methylates precorrin-2 at the C-20 position to produce
CC precorrin-3A. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=precorrin-2 + S-adenosyl-L-methionine = H(+) + precorrin-3A +
CC S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:16841, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:58561, ChEBI:CHEBI:58827,
CC ChEBI:CHEBI:59789; EC=2.1.1.130;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=precorrin-3B + S-adenosyl-L-methionine = 3 H(+) + precorrin-4
CC + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:12761, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57769, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:77870; EC=2.1.1.131;
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 1/10.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 3/10.
CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AE000516; AAK46406.1; -; Genomic_DNA.
DR PIR; E70764; E70764.
DR RefSeq; WP_003410659.1; NZ_KK341227.1.
DR AlphaFoldDB; P9WGB2; -.
DR SMR; P9WGB2; -.
DR EnsemblBacteria; AAK46406; AAK46406; MT2126.
DR KEGG; mtc:MT2126; -.
DR PATRIC; fig|83331.31.peg.2294; -.
DR HOGENOM; CLU_029920_1_0_11; -.
DR UniPathway; UPA00148; UER00212.
DR UniPathway; UPA00148; UER00214.
DR Proteomes; UP000001020; Chromosome.
DR GO; GO:0030788; F:precorrin-2 C20-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030789; F:precorrin-3B C17-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd11645; Precorrin_2_C20_MT; 1.
DR CDD; cd11646; Precorrin_3B_C17_MT; 1.
DR Gene3D; 3.30.950.10; -; 2.
DR Gene3D; 3.40.1010.10; -; 2.
DR InterPro; IPR000878; 4pyrrol_Mease.
DR InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR InterPro; IPR006363; Cbl_synth_CobJ/CibH_dom.
DR InterPro; IPR012382; CobI/CbiL.
DR InterPro; IPR006364; CobI/CbiL/CobIJ_dom.
DR InterPro; IPR003043; Uropor_MeTrfase_CS.
DR Pfam; PF00590; TP_methylase; 2.
DR SUPFAM; SSF53790; SSF53790; 2.
DR TIGRFAMs; TIGR01467; cobI_cbiL; 1.
DR TIGRFAMs; TIGR01466; cobJ_cbiH; 1.
DR PROSITE; PS00839; SUMT_1; 1.
DR PROSITE; PS00840; SUMT_2; 1.
PE 3: Inferred from homology;
KW Cobalamin biosynthesis; Methyltransferase; Multifunctional enzyme;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..508
FT /note="Cobalamin biosynthesis protein CobIJ"
FT /id="PRO_0000428392"
FT REGION 1..243
FT /note="Precorrin-2 C20-methyltransferase"
FT REGION 244..508
FT /note="Precorrin-3 methylase"
FT REGION 489..508
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 508 AA; 53911 MW; 95AC066F022C4DC1 CRC64;
MSARGTLWGV GLGPGDPELV TVKAARVIGE ADVVAYHSAP HGHSIARGIA EPYLRPGQLE
EHLVYPVTTE ATNHPGGYAG ALEDFYADAT ERIATHLDAG RNVALLAEGD PLFYSSYMHL
HTRLTRRFNA VIVPGVTSVS AASAAVATPL VAGDQVLSVL PGTLPVGELT RRLADADAAV
VVKLGRSYHN VREALSASGL LGDAFYVERA STAGQRVLPA ADVDETSVPY FSLAMLPGGR
RRALLTGTVA VVGLGPGDSD WMTPQSRREL AAATDLIGYR GYLDRVEVRD GQRRHPSDNT
DEPARARLAC SLADQGRAVA VVSSGDPGVF AMATAVLEEA EQWPGVRVRV IPAMTAAQAV
ASRVGAPLGH DYAVISLSDR LKPWDVIAAR LTAAAAADLV LAIYNPASVT RTWQVGAMRE
LLLAHRDPGI PVVIGRNVSG PVSGPNEDVR VVKLADLNPA EIDMRCLLIV GSSQTRWYSV
DSQDRVFTPR RYPEAGRATA TKSSRHSD