COBI_PSEAE
ID COBI_PSEAE Reviewed; 250 AA.
AC Q9HZU3;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Precorrin-2 C(20)-methyltransferase;
DE EC=2.1.1.130;
DE AltName: Full=S-adenosyl-L-methionine--precorrin-2 methyltransferase;
DE Short=SP2MT;
GN Name=cobI; OrderedLocusNames=PA2904;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Methylates precorrin-2 at the C-20 position to produce
CC precorrin-3A. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=precorrin-2 + S-adenosyl-L-methionine = H(+) + precorrin-3A +
CC S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:16841, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:58561, ChEBI:CHEBI:58827,
CC ChEBI:CHEBI:59789; EC=2.1.1.130;
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 1/10.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG06292.1; -; Genomic_DNA.
DR PIR; G83283; G83283.
DR RefSeq; NP_251594.1; NC_002516.2.
DR RefSeq; WP_003091011.1; NZ_QZGE01000009.1.
DR AlphaFoldDB; Q9HZU3; -.
DR SMR; Q9HZU3; -.
DR STRING; 287.DR97_5037; -.
DR PaxDb; Q9HZU3; -.
DR PRIDE; Q9HZU3; -.
DR EnsemblBacteria; AAG06292; AAG06292; PA2904.
DR GeneID; 882688; -.
DR KEGG; pae:PA2904; -.
DR PATRIC; fig|208964.12.peg.3045; -.
DR PseudoCAP; PA2904; -.
DR HOGENOM; CLU_076014_1_1_6; -.
DR InParanoid; Q9HZU3; -.
DR OMA; MEYPVTT; -.
DR PhylomeDB; Q9HZU3; -.
DR BioCyc; PAER208964:G1FZ6-2954-MON; -.
DR UniPathway; UPA00148; UER00212.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0030788; F:precorrin-2 C20-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR CDD; cd11645; Precorrin_2_C20_MT; 1.
DR Gene3D; 3.30.950.10; -; 1.
DR Gene3D; 3.40.1010.10; -; 1.
DR InterPro; IPR000878; 4pyrrol_Mease.
DR InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR InterPro; IPR012382; CobI/CbiL.
DR InterPro; IPR006364; CobI/CbiL/CobIJ_dom.
DR Pfam; PF00590; TP_methylase; 1.
DR PIRSF; PIRSF036427; Precrrn-2_mtase; 1.
DR SUPFAM; SSF53790; SSF53790; 1.
DR TIGRFAMs; TIGR01467; cobI_cbiL; 1.
PE 3: Inferred from homology;
KW Cobalamin biosynthesis; Methyltransferase; Reference proteome;
KW S-adenosyl-L-methionine; Transferase.
FT CHAIN 1..250
FT /note="Precorrin-2 C(20)-methyltransferase"
FT /id="PRO_0000287826"
SQ SEQUENCE 250 AA; 27275 MW; 3AC4B437C77F477E CRC64;
MMSVVKGRLL GLGVGPGDPE LITLKALRLL RAAPVVGYFV AKGKKGNAFG IIEAHLDQAQ
VRLPLVYPVT TEKLEPPLCY ETIISDFYDT AAEQLAAHLD AGRDVAVICE GDPFFYGSYM
YLHDRLATRY ESEVVPGVCS MLGGAAVLGA PLVYRNQSLS VLSGVLPEEE LRRRLADADA
AVVMKLGRNF DKVRRVLVEL GLERRALYVE RATMANQRIV PLERVEPMAS PYFSLIVVPG
DKWQGGAGGE