位置:首页 > 蛋白库 > COBJ_SINSX
COBJ_SINSX
ID   COBJ_SINSX              Reviewed;         254 AA.
AC   P21640;
DT   01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1991, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Precorrin-3B C(17)-methyltransferase;
DE            Short=Precorrin-3 methylase;
DE            Short=Precorrin-3 methyltransferase;
DE            EC=2.1.1.131;
GN   Name=cobJ;
OS   Sinorhizobium sp.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=42445;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SC510;
RX   PubMed=2211521; DOI=10.1128/jb.172.10.5980-5990.1990;
RA   Crouzet J., Cameron B., Cauchois L., Rigault S., Rouyez M.-C., Blanche F.,
RA   Thibaut D., Debussche L.;
RT   "Genetic and sequence analysis of an 8.7-kilobase Pseudomonas denitrificans
RT   fragment carrying eight genes involved in transformation of precorrin-2 to
RT   cobyrinic acid.";
RL   J. Bacteriol. 172:5980-5990(1990).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=8226690; DOI=10.1128/jb.175.22.7430-7440.1993;
RA   Debussche L., Thibaut D., Cameron B., Crouzet J., Blanche F.J.;
RT   "Biosynthesis of the corrin macrocycle of coenzyme B12 in Pseudomonas
RT   denitrificans.";
RL   J. Bacteriol. 175:7430-7440(1993).
CC   -!- FUNCTION: Methyltransferase that catalyzes the methylation of C-17 in
CC       precorrin-3B to form precorrin-4.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=precorrin-3B + S-adenosyl-L-methionine = 3 H(+) + precorrin-4
CC         + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:12761, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57769, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:77870; EC=2.1.1.131;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 3/10.
CC   -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to originate from Pseudomonas
CC       denitrificans, but similarity searches show that the sequence is much
CC       closer to Sinorhizobium. The entry's taxonomy has been changed.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; M59301; AAA25798.1; -; Genomic_DNA.
DR   AlphaFoldDB; P21640; -.
DR   SMR; P21640; -.
DR   KEGG; ag:AAA25798; -.
DR   BioCyc; MetaCyc:MON-85; -.
DR   UniPathway; UPA00148; UER00214.
DR   GO; GO:0030789; F:precorrin-3B C17-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   CDD; cd11646; Precorrin_3B_C17_MT; 1.
DR   Gene3D; 3.30.950.10; -; 1.
DR   Gene3D; 3.40.1010.10; -; 1.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR   InterPro; IPR006363; Cbl_synth_CobJ/CibH_dom.
DR   Pfam; PF00590; TP_methylase; 1.
DR   SUPFAM; SSF53790; SSF53790; 1.
DR   TIGRFAMs; TIGR01466; cobJ_cbiH; 1.
PE   1: Evidence at protein level;
KW   Cobalamin biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..254
FT                   /note="Precorrin-3B C(17)-methyltransferase"
FT                   /id="PRO_0000150402"
SQ   SEQUENCE   254 AA;  27105 MW;  A30B027F4AFBE23F CRC64;
     MTGTLYVVGT GPGSAKQMTP ETAEAVAAAQ EFYGYFPYLD RLNLRPDQIR VASDNREELD
     RAQVALTRAA AGVKVCMVSG GDPGVFAMAA AVCEAIDKGP AEWKSVELVI TPGVTAMLAV
     AARIGAPLGH DFCAISLSDN LKPWEVITRR LRLAAEAGFV IALYNPISKA RPWQLGEAFE
     LLRSVLPASV PVIFGRAAGR PDERIAVMPL GEADANRADM ATCVIIGSPE TRIVERDGQP
     DLVYTPRFYA GASQ
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024