ID   COBK_MYCTO              Reviewed;         244 AA.
AC   P9WP88; L0TBE4; Q10680;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 31.
DE   RecName: Full=Precorrin-6A reductase;
DE            EC=1.3.1.54;
DE   AltName: Full=Precorrin-6X reductase;
GN   Name=cobK; OrderedLocusNames=MT2130;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Catalyzes the reduction of the macrocycle of precorrin-6X
CC       into precorrin-6Y. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + precorrin-6B = 2 H(+) + NADPH + precorrin-6A;
CC         Xref=Rhea:RHEA:23408, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:58532, ChEBI:CHEBI:77872; EC=1.3.1.54;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 6/10.
CC   -!- SIMILARITY: Belongs to the precorrin-6x reductase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00356}.
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DR   EMBL; AE000516; AAK46410.1; -; Genomic_DNA.
DR   PIR; A70765; A70765.
DR   RefSeq; WP_003900455.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WP88; -.
DR   SMR; P9WP88; -.
DR   EnsemblBacteria; AAK46410; AAK46410; MT2130.
DR   KEGG; mtc:MT2130; -.
DR   PATRIC; fig|83331.31.peg.2298; -.
DR   HOGENOM; CLU_068627_1_0_11; -.
DR   UniPathway; UPA00148; UER00217.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016994; F:precorrin-6A reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003723; Precorrin-6x_reduct.
DR   PANTHER; PTHR36925; PTHR36925; 1.
DR   Pfam; PF02571; CbiJ; 1.
DR   TIGRFAMs; TIGR00715; precor6x_red; 1.
DR   PROSITE; PS51014; COBK_CBIJ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; NADP; Oxidoreductase.
FT   CHAIN           1..244
FT                   /note="Precorrin-6A reductase"
FT                   /id="PRO_0000426994"
SQ   SEQUENCE   244 AA;  25737 MW;  946A3F5E7740807C CRC64;
     MTRVLLLGGT AEGRALAKEL HPHVEIVSSL AGRVPNPALP IGPVRIGGFG GVEGLRGWLR
     EERIDAVVDA THPFAVTITA HAAQVCGELG LPYLVLARPP WDPGTAIIAV SDIEAADVVA
     EQGYSRVFLT TGRSGIAAFA NSDAWFLIRV VTAPDGTALP RRHKLVLSRG PYGYHDEFAL
     LREQRIDALV TKNSGGKMTR AKLDAAAALG ISVVMIARPL LPAGVAAVDS VHRAAMWVAG
     LPSR