ID   COBK_RHOER              Reviewed;         248 AA.
AC   Q53139;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Precorrin-6A reductase;
DE            EC=1.3.1.54;
DE   AltName: Full=Precorrin-6X reductase;
GN   Name=cobK;
OS   Rhodococcus erythropolis (Arthrobacter picolinophilus).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus;
OC   Rhodococcus erythropolis group.
OX   NCBI_TaxID=1833;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NI86/21;
RX   PubMed=8200543; DOI=10.1016/0378-1119(94)90610-6;
RA   de Mot R., Nagy I., Schoofs G., Vanderleyden J.;
RT   "Sequences of the cobalamin biosynthetic genes cobK, cobL and cobM from
RT   Rhodococcus sp. NI86/21.";
RL   Gene 143:91-93(1994).
CC   -!- FUNCTION: Catalyzes the reduction of the macrocycle of precorrin-6X
CC       into precorrin-6Y.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + precorrin-6B = 2 H(+) + NADPH + precorrin-6A;
CC         Xref=Rhea:RHEA:23408, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58349, ChEBI:CHEBI:58532, ChEBI:CHEBI:77872; EC=1.3.1.54;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 6/10.
CC   -!- SIMILARITY: Belongs to the precorrin-6x reductase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00356}.
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DR   EMBL; L21196; AAC37131.1; -; Genomic_DNA.
DR   PIR; T46837; T46837.
DR   AlphaFoldDB; Q53139; -.
DR   SMR; Q53139; -.
DR   UniPathway; UPA00148; UER00217.
DR   GO; GO:0016994; F:precorrin-6A reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003723; Precorrin-6x_reduct.
DR   PANTHER; PTHR36925; PTHR36925; 1.
DR   Pfam; PF02571; CbiJ; 1.
DR   TIGRFAMs; TIGR00715; precor6x_red; 1.
DR   PROSITE; PS51014; COBK_CBIJ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; NADP; Oxidoreductase.
FT   CHAIN           1..248
FT                   /note="Precorrin-6A reductase"
FT                   /id="PRO_0000135922"
SQ   SEQUENCE   248 AA;  26142 MW;  2BA2B2D6810C4623 CRC64;
     MTRILILGGT GEARALAAAL ADVPGVEAVS SLAGRVRDPR LPVGDVRIGG FGGADGLAEC
     VRAHPVDAIV DATHPFAAQI TRNAADAAHR RGIPLVVLRR PEWSPRPGEH WHGAADLADA
     AELLPDLGTR IFLTIGRQGV DAFADLQALW FLIRAIDPPD VAMPPHSTLL LARGPFAVAD
     ETALMREHRI DVLVTKNSGG GQTDAKLDAA RALGIPVLMI RRPPLPPATE TVDDVAGAIA
     WVGTLTRR