ID   COBL_MYCTO              Reviewed;         390 AA.
AC   P9WGA8; L0TA32; Q10671;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Precorrin-6Y C(5,15)-methyltransferase [decarboxylating];
DE            Short=Precorrin-6 methyltransferase;
DE            Short=Precorrin-6Y methylase;
DE            EC=2.1.1.132;
GN   Name=cobL; OrderedLocusNames=MT2132;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Catalyzes the methylation of both C-5 and C-15 in precorrin-
CC       6Y to form precorrin-8X. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=precorrin-6B + 2 S-adenosyl-L-methionine = CO2 + 3 H(+) +
CC         precorrin-8X + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:17477,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:58532, ChEBI:CHEBI:58581, ChEBI:CHEBI:59789;
CC         EC=2.1.1.132;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 7/10.
CC   -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000516; AAK46412.1; -; Genomic_DNA.
DR   PIR; C70765; C70765.
DR   RefSeq; WP_010924485.1; NC_002755.2.
DR   AlphaFoldDB; P9WGA8; -.
DR   SMR; P9WGA8; -.
DR   EnsemblBacteria; AAK46412; AAK46412; MT2132.
DR   KEGG; mtc:MT2132; -.
DR   HOGENOM; CLU_031955_0_0_11; -.
DR   UniPathway; UPA00148; UER00218.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0046025; F:precorrin-6Y C5,15-methyltransferase (decarboxylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008276; F:protein methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd11644; Precorrin-6Y-MT; 1.
DR   Gene3D; 3.40.1010.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR012818; CbiE.
DR   InterPro; IPR006365; Cbl_synth_CobL.
DR   InterPro; IPR014008; Cbl_synth_MTase_CbiT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF00590; TP_methylase; 1.
DR   PIRSF; PIRSF036428; CobL; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF53790; SSF53790; 1.
DR   TIGRFAMs; TIGR02467; CbiE; 1.
DR   TIGRFAMs; TIGR02469; CbiT; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN           1..390
FT                   /note="Precorrin-6Y C(5,15)-methyltransferase
FT                   [decarboxylating]"
FT                   /id="PRO_0000428394"
SQ   SEQUENCE   390 AA;  41860 MW;  E04EB8B046637DFF CRC64;
     MIIVVGIGAD GMTGLSEHSR SELRRATVIY GSKRQLALLD DTVTAERWEW PTPMLPAVQG
     LSPDGADLHV VASGDPLLHG IGSTLIRLFG HDNVTVLPHV SAVTLACARM GWNVYDTEVI
     SLVTAQPHTA VRRGGRAIVL SGDRSTPQAL AVLLTEHGRG DSKFSVLEQL GGPAERRRDG
     TARAWACDPP LDVDELNVIA VRYLPDERTS WAPDEAFAHD GQITKHPIRV LTLAALAPRP
     GQRLWDVGAG SGAIAVQWCR SWPGCTAVAF ERDERRRRNI GFNAAAFGVS VDVRGDAPDA
     FDDAARPSVI FLGGGVTQPG LLEACLHSLP AGGNLVANAV TVESEAALAH AYSRLGGELR
     RFQHYLGEPL GGFTGWRPQL PVTQWSVTKR