ID   COBL_MYCTU              Reviewed;         390 AA.
AC   P9WGA9; L0TA32; Q10671;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Precorrin-6Y C(5,15)-methyltransferase [decarboxylating];
DE            Short=Precorrin-6 methyltransferase;
DE            Short=Precorrin-6Y methylase;
DE            EC=2.1.1.132;
GN   Name=cobL; OrderedLocusNames=Rv2072c; ORFNames=MTCY49.11c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Catalyzes the methylation of both C-5 and C-15 in precorrin-
CC       6Y to form precorrin-8X. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=precorrin-6B + 2 S-adenosyl-L-methionine = CO2 + 3 H(+) +
CC         precorrin-8X + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:17477,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:58532, ChEBI:CHEBI:58581, ChEBI:CHEBI:59789;
CC         EC=2.1.1.132;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 7/10.
CC   -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP44846.1; -; Genomic_DNA.
DR   PIR; C70765; C70765.
DR   RefSeq; NP_216588.1; NC_000962.3.
DR   RefSeq; WP_003911740.1; NZ_NVQJ01000047.1.
DR   AlphaFoldDB; P9WGA9; -.
DR   SMR; P9WGA9; -.
DR   STRING; 83332.Rv2072c; -.
DR   PaxDb; P9WGA9; -.
DR   DNASU; 888321; -.
DR   GeneID; 888321; -.
DR   KEGG; mtu:Rv2072c; -.
DR   TubercuList; Rv2072c; -.
DR   eggNOG; COG2241; Bacteria.
DR   eggNOG; COG2242; Bacteria.
DR   OMA; MPVTQWS; -.
DR   PhylomeDB; P9WGA9; -.
DR   UniPathway; UPA00148; UER00218.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0046025; F:precorrin-6Y C5,15-methyltransferase (decarboxylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008276; F:protein methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd11644; Precorrin-6Y-MT; 1.
DR   Gene3D; 3.40.1010.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR012818; CbiE.
DR   InterPro; IPR006365; Cbl_synth_CobL.
DR   InterPro; IPR014008; Cbl_synth_MTase_CbiT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF00590; TP_methylase; 1.
DR   PIRSF; PIRSF036428; CobL; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF53790; SSF53790; 1.
DR   TIGRFAMs; TIGR02467; CbiE; 1.
DR   TIGRFAMs; TIGR02469; CbiT; 1.
PE   1: Evidence at protein level;
KW   Cobalamin biosynthesis; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..390
FT                   /note="Precorrin-6Y C(5,15)-methyltransferase
FT                   [decarboxylating]"
FT                   /id="PRO_0000150409"
SQ   SEQUENCE   390 AA;  41854 MW;  FB42EFF7562F21F3 CRC64;
     MIIVVGIGAD GMTGLSEHSR SELRRATVIY GSKRQLALLD DTVTAERWEW PTPMLPAVQG
     LSPDGADLHV VASGDPLLHG IGSTLIRLFG HDNVTVLPHV SAVTLACARM GWNVYDTEVI
     SLVTAQPHTA VRRGGRAIVL SGDRSTPQAL AVLLTEHGRG DSKFSVLEQL GGPAERRRDG
     TARAWACDPP LDVDELNVIA VRYLLDERTS WAPDEAFAHD GQITKHPIRV LTLAALAPRP
     GQRLWDVGAG SGAIAVQWCR SWPGCTAVAF ERDERRRRNI GFNAAAFGVS VDVRGDAPDA
     FDDAARPSVI FLGGGVTQPG LLEACLDSLP AGGNLVANAV TVESEAALAH AYSRLGGELR
     RFQHYLGEPL GGFTGWRPQL PVTQWSVTKR