ID   COBL_PSEAE              Reviewed;         415 AA.
AC   Q9HZU0;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Precorrin-6Y C(5,15)-methyltransferase [decarboxylating];
DE            Short=Precorrin-6 methyltransferase;
DE            Short=Precorrin-6Y methylase;
DE            EC=2.1.1.132;
GN   Name=cobL; OrderedLocusNames=PA2907;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the methylation of both C-5 and C-15 in precorrin-
CC       6Y to form precorrin-8X. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=precorrin-6B + 2 S-adenosyl-L-methionine = CO2 + 3 H(+) +
CC         precorrin-8X + 2 S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:17477,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:58532, ChEBI:CHEBI:58581, ChEBI:CHEBI:59789;
CC         EC=2.1.1.132;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 7/10.
CC   -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG06295.1; -; Genomic_DNA.
DR   PIR; E83281; E83281.
DR   RefSeq; NP_251597.1; NC_002516.2.
DR   RefSeq; WP_003114023.1; NZ_QZGE01000009.1.
DR   AlphaFoldDB; Q9HZU0; -.
DR   SMR; Q9HZU0; -.
DR   STRING; 287.DR97_5034; -.
DR   PaxDb; Q9HZU0; -.
DR   PRIDE; Q9HZU0; -.
DR   EnsemblBacteria; AAG06295; AAG06295; PA2907.
DR   GeneID; 882678; -.
DR   KEGG; pae:PA2907; -.
DR   PATRIC; fig|208964.12.peg.3048; -.
DR   PseudoCAP; PA2907; -.
DR   HOGENOM; CLU_031955_0_0_6; -.
DR   InParanoid; Q9HZU0; -.
DR   OMA; MPVTQWS; -.
DR   PhylomeDB; Q9HZU0; -.
DR   BioCyc; PAER208964:G1FZ6-2957-MON; -.
DR   UniPathway; UPA00148; UER00218.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0046025; F:precorrin-6Y C5,15-methyltransferase (decarboxylating) activity; IEA:UniProtKB-EC.
DR   GO; GO:0008276; F:protein methyltransferase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd11644; Precorrin-6Y-MT; 1.
DR   Gene3D; 3.40.1010.10; -; 1.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR012818; CbiE.
DR   InterPro; IPR006365; Cbl_synth_CobL.
DR   InterPro; IPR014008; Cbl_synth_MTase_CbiT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   Pfam; PF00590; TP_methylase; 1.
DR   PIRSF; PIRSF036428; CobL; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
DR   SUPFAM; SSF53790; SSF53790; 1.
DR   TIGRFAMs; TIGR02467; CbiE; 1.
DR   TIGRFAMs; TIGR02469; CbiT; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..415
FT                   /note="Precorrin-6Y C(5,15)-methyltransferase
FT                   [decarboxylating]"
FT                   /id="PRO_0000287737"
SQ   SEQUENCE   415 AA;  44128 MW;  BF3E02C043B47811 CRC64;
     MSSADSTPPW LTLVGIGEDG YPGLGKQARR ALLQASRIVG AARQLELLPP CIGAARETWP
     TPFSLEPLLA RRGQPTCVLA SGDPMLFGVG ASLARQLPAT ELRVLPAPSS LSLAAARLGW
     ALQEVACLSL VARPLAALQA QVHDGRRLLV LSNDGDSPAA IARLLNARGF GASRLSVLEH
     LGGPLERRLD GLAGDWSLPR AADLNLVAVE CRAGAGAVRL PLTPGLADEA YRHDGQLTKR
     DVRAVTLARL APCPGELLWD VGAGCGSIGI EWMRAHPSCR AIAIEANDER QEHIRHNRDA
     LGVPTLHLVA GSAPEALLEL PEPDAIFIGG GVTAEGVLET CWERLRPGGR LLANAVTLQS
     ERVLLDWQQR LGGDLTRLGV AHARPLGGFD TWRQALPITL LELRKPAARK PPRDA