ID   COBM_PSEAE              Reviewed;         250 AA.
AC   Q9HZP9;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Precorrin-4 C(11)-methyltransferase;
DE            EC=2.1.1.133;
DE   AltName: Full=Precorrin-3 methylase;
GN   Name=cobM; OrderedLocusNames=PA2948;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Catalyzes the methylation of C-11 in precorrin-4 to form
CC       precorrin-5. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=precorrin-4 + S-adenosyl-L-methionine = precorrin-5 + S-
CC         adenosyl-L-homocysteine; Xref=Rhea:RHEA:22012, ChEBI:CHEBI:57769,
CC         ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:77871;
CC         EC=2.1.1.133;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from precorrin-2 (aerobic route): step 4/10.
CC   -!- SIMILARITY: Belongs to the precorrin methyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG06336.1; -; Genomic_DNA.
DR   PIR; G83276; G83276.
DR   RefSeq; NP_251638.1; NC_002516.2.
DR   RefSeq; WP_003102926.1; NZ_QZGE01000009.1.
DR   AlphaFoldDB; Q9HZP9; -.
DR   SMR; Q9HZP9; -.
DR   STRING; 287.DR97_4991; -.
DR   PaxDb; Q9HZP9; -.
DR   PRIDE; Q9HZP9; -.
DR   EnsemblBacteria; AAG06336; AAG06336; PA2948.
DR   GeneID; 882854; -.
DR   KEGG; pae:PA2948; -.
DR   PATRIC; fig|208964.12.peg.3094; -.
DR   PseudoCAP; PA2948; -.
DR   HOGENOM; CLU_011276_7_1_6; -.
DR   InParanoid; Q9HZP9; -.
DR   OMA; WSAMGEQ; -.
DR   PhylomeDB; Q9HZP9; -.
DR   BioCyc; PAER208964:G1FZ6-2999-MON; -.
DR   UniPathway; UPA00148; UER00215.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0046026; F:precorrin-4 C11-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   CDD; cd11641; Precorrin-4_C11-MT; 1.
DR   Gene3D; 3.30.950.10; -; 1.
DR   Gene3D; 3.40.1010.10; -; 1.
DR   InterPro; IPR000878; 4pyrrol_Mease.
DR   InterPro; IPR035996; 4pyrrol_Methylase_sf.
DR   InterPro; IPR014777; 4pyrrole_Mease_sub1.
DR   InterPro; IPR014776; 4pyrrole_Mease_sub2.
DR   InterPro; IPR006362; Cbl_synth_CobM/CibF.
DR   InterPro; IPR003043; Uropor_MeTrfase_CS.
DR   Pfam; PF00590; TP_methylase; 1.
DR   SUPFAM; SSF53790; SSF53790; 1.
DR   TIGRFAMs; TIGR01465; cobM_cbiF; 1.
DR   PROSITE; PS00839; SUMT_1; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Methyltransferase; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..250
FT                   /note="Precorrin-4 C(11)-methyltransferase"
FT                   /id="PRO_0000287735"
SQ   SEQUENCE   250 AA;  27040 MW;  6E40E1D1F6F38FBD CRC64;
     MTVYFIGAGP GDPDLITVKG QRLIRQCPVI LYAGSLVPQA LLEGHQAGQV VNTAELDLEQ
     IVELLAQAHR RGLDVARVHS GDPSLYGAIG EQIRHLRELG IPYEIVPGVT ATAACAALLG
     CELTLPEVSQ TLILTRYAAR TKMPEGESLG DLARHRATLA IHLGVAHLAK IVEELLPHYG
     ADCPVAVIHR ASWPDQEQVR GTLGDILPKV AARNFRRTAL ILVGEVLAAE GFADSSLYRA
     EHRHLYRPGE