ID   COBQ_ARCFU              Reviewed;         464 AA.
AC   O28931;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Probable cobyric acid synthase;
GN   Name=cobQ; Synonyms=cbiP; OrderedLocusNames=AF_1338;
OS   Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS   100126 / VC-16).
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC   Archaeoglobus.
OX   NCBI_TaxID=224325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX   PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA   Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA   Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA   Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA   Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA   Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA   Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA   Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA   Smith H.O., Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT   archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation (By
CC       similarity). {ECO:0000250}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AE000782; AAB89906.1; -; Genomic_DNA.
DR   PIR; A69417; A69417.
DR   RefSeq; WP_010878835.1; NC_000917.1.
DR   AlphaFoldDB; O28931; -.
DR   STRING; 224325.AF_1338; -.
DR   EnsemblBacteria; AAB89906; AAB89906; AF_1338.
DR   GeneID; 24794950; -.
DR   KEGG; afu:AF_1338; -.
DR   eggNOG; arCOG00105; Archaea.
DR   HOGENOM; CLU_019250_2_2_2; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 34382at2157; -.
DR   PhylomeDB; O28931; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000002199; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..464
FT                   /note="Probable cobyric acid synthase"
FT                   /id="PRO_0000141344"
FT   DOMAIN          245..427
FT                   /note="GATase cobBQ-type"
FT   ACT_SITE        321
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        419
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   464 AA;  51312 MW;  7DDC6D0A8193E0C4 CRC64;
     MPSLMVGGTT SSAGKSLLAA AFCRILARRG YDVAPFKAQN MSLNSFVTSK GKEIAIAQAY
     QAFAAGIEPD ERMNPVLLKP KGNFVSQLVV MGEAVGDVDS RKYYGVKVEW LKRVVEEAYL
     SLAEEYDFVV IEGAGGMAEI NLYERDLPNI HIARFARPDI LIVGDIDRGG VFASLYGTYA
     LLPDDVKPLV KGFVINRLRG REDVLESGIR ELERLTGIRV LGVLPYLDYN FPSEDSLNIE
     EWGAEGTVGI VRLPRVSNFT DFEPLREHAR FLSLNSSLNG CEVVIIPGSK DTIADLKALK
     SSKLGEEIVR KAGEIPVIGI CGGYQIMCRE LVDMGVEHGR IRAKGLGLLD AVTEFREYRK
     RTVQVEKRVN GNAVILDRIR GEKVWGYEIH KGITRASNPI FEDDGCASED GMCWGTYLHG
     LFWNENVLRA LGGYLGIKFR QKEDWADLIA DEVEGRLDLG VLGL