ID   COBQ_BRADU              Reviewed;         482 AA.
AC   Q89Q71;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=bll3259;
OS   Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS   NBRC 14792 / USDA 110).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=224911;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX   PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA   Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA   Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA   Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT   "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT   Bradyrhizobium japonicum USDA110.";
RL   DNA Res. 9:189-197(2002).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; BA000040; BAC48524.1; -; Genomic_DNA.
DR   RefSeq; NP_769899.1; NC_004463.1.
DR   RefSeq; WP_011086043.1; NZ_CP011360.1.
DR   AlphaFoldDB; Q89Q71; -.
DR   SMR; Q89Q71; -.
DR   STRING; 224911.27351518; -.
DR   EnsemblBacteria; BAC48524; BAC48524; BAC48524.
DR   GeneID; 64023009; -.
DR   KEGG; bja:bll3259; -.
DR   PATRIC; fig|224911.44.peg.2904; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_5; -.
DR   InParanoid; Q89Q71; -.
DR   OMA; EIHHGVA; -.
DR   PhylomeDB; Q89Q71; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000002526; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..482
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000141289"
FT   DOMAIN          249..436
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        331
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        428
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   482 AA;  51280 MW;  D455C166E384AA05 CRC64;
     MARALMIQGA GSDVGKSLIV AGLARAFTRR GLRVLPFKPQ NMSNNAAVTV DGGEIGRAQA
     LQALAAGVEP HTDMNPVLLK PETDVGAQVV VHGKRIATAR AREYAAMKPS LMGAVLESFE
     RLKARADLVL VEGAGSPAEV NLRKADIANM GFARKADVPV VLIGDIDRGG VIAQLVGIKT
     VIDPDDAAMI QGFVINKFRG DPTLFDDGYK LIEAKTAWRG FGVLPWFARA GELPAEDALG
     LSDARKPGQC KIACLALSRI ANFDDLDPLK LEAAVDLVMV RPGEAIPGDV RLVIIPGSKS
     TRGDLAFLRA QGWDIDLLAH YRRGGHVLGL CGGYQMLGRS VADPDGIEGP AGDTPGLGLL
     DVQTVMSPQK TLTRVTAVHA ATNQPIQAYE IHIGRTDGPD RARPFAKLNG EPEGAISSDG
     RVQGSYLHGL FTSDDFRKAF LTKLDIPAGD EPYHSRVESA LDALADHIEK HLDVEGLLSL
     AR