ID   COBQ_CHLPD              Reviewed;         505 AA.
AC   A1BFI0;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028};
GN   OrderedLocusNames=Cpha266_1117;
OS   Chlorobium phaeobacteroides (strain DSM 266).
OC   Bacteria; Chlorobi; Chlorobia; Chlorobiales; Chlorobiaceae;
OC   Chlorobium/Pelodictyon group; Chlorobium.
OX   NCBI_TaxID=290317;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 266;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T.,
RA   Overmann J., Bryant D.A., Richardson P.;
RT   "Complete sequence of Chlorobium phaeobacteroides DSM 266.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; CP000492; ABL65157.1; -; Genomic_DNA.
DR   RefSeq; WP_011744983.1; NC_008639.1.
DR   AlphaFoldDB; A1BFI0; -.
DR   SMR; A1BFI0; -.
DR   STRING; 290317.Cpha266_1117; -.
DR   EnsemblBacteria; ABL65157; ABL65157; Cpha266_1117.
DR   KEGG; cph:Cpha266_1117; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_10; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 744477at2; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000008701; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..505
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000332327"
FT   DOMAIN          260..453
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        341
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        445
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   505 AA;  54532 MW;  ABF20459E2CAB5C2 CRC64;
     MVNSSPVPGA LAIFGTASDV GKSIVATALC RMFSNAGIDV APYKAQNMSN NSGVTPDGCE
     IGRAQIAQAE AARVVPTADM NPVLLKPNSD TGAQIVLQGK VCSTETAKGY FLDTSLWAEA
     ARKSLDQLMQ RHELVVIEGA GSCAEMNLYD RDFVNFRTAR ISGAPVILVA DIDRGGVFAQ
     VVGTLAVLPP EDRALVKGVI INRFRGDIDL FRDGVKLIET LAGIPVLGVI PYFRGFRIDA
     EDAVPLSSKV DPSGAPEKGR IAVAAIYFPH ISNFTDLSPL ELDPKVELHY LHFPRSLRGY
     QALILPGTKN VRGDLDWLTS LGWAEKIREF RRDGGLIMGI CGGYQMLGAT IADPSGVEGE
     PGESAGLGML PVHTVLEEEK CLSNAIGNIQ GESIAVSGYE IHMGRTTSNG DCSSFLRVTA
     RNNRPADDVD GVITPDGKVI GTYFHGIIDE PEVRCWFLRQ IDPAYTPDAE ERGRQESYDL
     LADHFSGYLD IPKLYEIIQR PCPNP