ID   COBQ_DEIRA              Reviewed;         474 AA.
AC   Q9RZU9;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 2.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=DR_B0012;
OS   Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG
OS   4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).
OG   Plasmid megaplasmid MP1.
OC   Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae;
OC   Deinococcus.
OX   NCBI_TaxID=243230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA   White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA   Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA   Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA   Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA   Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA   Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA   Fraser C.M.;
RT   "Genome sequence of the radioresistant bacterium Deinococcus radiodurans
RT   R1.";
RL   Science 286:1571-1577(1999).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF12574.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE001826; AAF12574.1; ALT_INIT; Genomic_DNA.
DR   PIR; D75619; D75619.
DR   RefSeq; NP_051555.1; NC_000958.1.
DR   RefSeq; WP_027480394.1; NC_000958.1.
DR   AlphaFoldDB; Q9RZU9; -.
DR   SMR; Q9RZU9; -.
DR   PRIDE; Q9RZU9; -.
DR   EnsemblBacteria; AAF12574; AAF12574; DR_B0012.
DR   KEGG; dra:DR_B0012; -.
DR   PATRIC; fig|243230.17.peg.10; -.
DR   HOGENOM; CLU_019250_2_2_0; -.
DR   InParanoid; Q9RZU9; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 744477at2; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000002524; Plasmid megaplasmid MP1.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Plasmid;
KW   Reference proteome.
FT   CHAIN           1..474
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000141301"
FT   DOMAIN          251..431
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        328
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        423
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   474 AA;  50363 MW;  B671B11DBFA8E3C8 CRC64;
     MVQGCTSNAG KSYLCAALCR MLSDEGLRVA PFKAQNMSNN AGVTPPESSA PGLEMGRAQL
     VQARAARVVP DVRMNPVLLK PEADTSSQVV LLGRAAPELT ALPWRERKPR LWPFVKGALH
     SLLDEFDVVV IEGAGSPAEV NLRPSDIVNM RVAREARAAV LLAADIDRGG AFAHLLGTWH
     CLMPEERALM RGFILNRFRG DASLLAPAPE WLEEQTGVPT LGVVPWLNVA LPEEDGVAVE
     APAAIIPSPS TGFVAIPRLP RVSNLDEFAP LGELARWVTS PQELAGARAV ILPGSKSTAA
     DLAWLRATGL AGAVTRLAVQ GVPVLGICGG LQMLGQTLSD PQGVEGGSAA SGGKVYGLGL
     LDLHTAFAAD KTTRLSEVRD PETGLRLQGY EIHHGQTRSG PGVQTLVPGL LWRSGNVRGT
     YLHGLLENSA YLEHFLRWAD LPVPACLDSL DARLDAIAAQ VQAGLSWDRV RALL