ID   COBQ_DESHY              Reviewed;         514 AA.
AC   Q24Q41;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=DSY4062;
OS   Desulfitobacterium hafniense (strain Y51).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC   Desulfitobacterium.
OX   NCBI_TaxID=138119;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Y51;
RX   PubMed=16513756; DOI=10.1128/jb.188.6.2262-2274.2006;
RA   Nonaka H., Keresztes G., Shinoda Y., Ikenaga Y., Abe M., Naito K.,
RA   Inatomi K., Furukawa K., Inui M., Yukawa H.;
RT   "Complete genome sequence of the dehalorespiring bacterium
RT   Desulfitobacterium hafniense Y51 and comparison with Dehalococcoides
RT   ethenogenes 195.";
RL   J. Bacteriol. 188:2262-2274(2006).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; AP008230; BAE85851.1; -; Genomic_DNA.
DR   RefSeq; WP_005812308.1; NC_007907.1.
DR   AlphaFoldDB; Q24Q41; -.
DR   SMR; Q24Q41; -.
DR   STRING; 138119.DSY4062; -.
DR   EnsemblBacteria; BAE85851; BAE85851; DSY4062.
DR   KEGG; dsy:DSY4062; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_9; -.
DR   OMA; EIHHGVA; -.
DR   OrthoDB; 744477at2; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000001946; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..514
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000332337"
FT   DOMAIN          263..457
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        344
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        449
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   514 AA;  56682 MW;  66F498CF789DD675 CRC64;
     MSGKKAAARL MIQGTSSNVG KSVLAAAFCR IFYQEGYHVA PFKAQNMALN SFITRSGGEM
     GRAQVVQAQA AGLEPDVRMN PVLLKPTGHT GSQVIVLGKA QGTLSALKYH GDYQRKTWPI
     VEEALHELLE EHEIVVIEGA GSPAEVNLKQ NDIVNMRVAK EAQAPVLLVA DIDRGGALAS
     VVGTLELLEP EERQLVQGIV MNKFRGDIKL LQPALDFLEE RTGIPVVGVV PFYDQFKIPE
     EDSVVLEEQN TSKRDQGKSR DEALDVAVIR LPYLSNFTDF DPFEDEPDVI LRYVREPSEL
     GEPDCVIIPG SKNTLSDLRF LWESGLGEKI IKFWQEDVPI IGICGGYQML GRIVRDPLGT
     ESDLKEIAGL GILPMETEFE LDKHTVQSRG KVVGGELFLA RCQGTEITGY EIHMGRSQGE
     GHSLFEIEAQ ETAYGDGMSA GSAVGTYLHG IFDNDPLRTA LLEWLWERRG GTRPVGETMS
     QAAIREQSFN ELADWVRNSM DMEKIRAMMG LHKG