ID   COBQ_DESVH              Reviewed;         543 AA.
AC   Q72DW3;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; OrderedLocusNames=DVU_0816;
OS   Desulfovibrio vulgaris (strain ATCC 29579 / DSM 644 / NCIMB 8303 / VKM
OS   B-1760 / Hildenborough).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfovibrionales;
OC   Desulfovibrionaceae; Desulfovibrio.
OX   NCBI_TaxID=882;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29579 / DSM 644 / NCIMB 8303 / VKM B-1760 / Hildenborough;
RX   PubMed=15077118; DOI=10.1038/nbt959;
RA   Heidelberg J.F., Seshadri R., Haveman S.A., Hemme C.L., Paulsen I.T.,
RA   Kolonay J.F., Eisen J.A., Ward N.L., Methe B.A., Brinkac L.M.,
RA   Daugherty S.C., DeBoy R.T., Dodson R.J., Durkin A.S., Madupu R.,
RA   Nelson W.C., Sullivan S.A., Fouts D.E., Haft D.H., Selengut J.,
RA   Peterson J.D., Davidsen T.M., Zafar N., Zhou L., Radune D., Dimitrov G.,
RA   Hance M., Tran K., Khouri H.M., Gill J., Utterback T.R., Feldblyum T.V.,
RA   Wall J.D., Voordouw G., Fraser C.M.;
RT   "The genome sequence of the anaerobic, sulfate-reducing bacterium
RT   Desulfovibrio vulgaris Hildenborough.";
RL   Nat. Biotechnol. 22:554-559(2004).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; AE017285; AAS95296.1; -; Genomic_DNA.
DR   RefSeq; WP_010938117.1; NC_002937.3.
DR   RefSeq; YP_010037.1; NC_002937.3.
DR   AlphaFoldDB; Q72DW3; -.
DR   STRING; 882.DVU_0816; -.
DR   PaxDb; Q72DW3; -.
DR   EnsemblBacteria; AAS95296; AAS95296; DVU_0816.
DR   KEGG; dvu:DVU_0816; -.
DR   PATRIC; fig|882.5.peg.766; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_7; -.
DR   OMA; EIHHGVA; -.
DR   PhylomeDB; Q72DW3; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000002194; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..543
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000141302"
FT   DOMAIN          260..483
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        346
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        475
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   543 AA;  57058 MW;  DE4636EDA903B941 CRC64;
     MKSPTPALML QGTSSNAGKS ILTAAFCRIL LQDGYRVAPF KAQNMALNSH VTADGLELGR
     AQAVQAAACR LDVDVRMNPV LLKPNSDTGS QVVVMGRPVG NMRVREYMAY KPVAFDAARA
     AYDSLAADVD VMVLEGAGSP AEVNLKAHDI VNMAMARHAE AKVLLVGDID RGGVFASLVG
     TMELLDPWER DLVAGFVLNK FRGDATLLSP AYDVVTGRTG KPFLGVVPWL HDLGLPDEDS
     VSFRETLAGR APDVPAGGGM LDIVLVDLPH ISNFTDLDAL RREPDVAVRV VRSPEQLGSP
     DAIILPGSKN TLGDLAALRS RGMAEALCAL RSAEGGHLGP VIVGICGGFQ MMGRFLADPQ
     GVESDHACTE AGLGLLPVTT EMGSAKVLRR TSAKTVSGWC GAGVAAGLMP GTGGGHGGAE
     VCPGEGYAVH GYEIHHGVTT PDAGGGAVLV CLHESDGSPA GWTTPDGQVW GTYLHGVFDA
     DGFRRGWLDA LRVRRGLEPV GRVVARYDLD PALDRLADVV REAVDMGHIY SVLGLSPKGR
     RQT