COBQ_FRACC
ID COBQ_FRACC Reviewed; 520 AA.
AC Q2J9S7;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 07-MAR-2006, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028};
GN OrderedLocusNames=Francci3_2603;
OS Frankia casuarinae (strain DSM 45818 / CECT 9043 / CcI3).
OC Bacteria; Actinobacteria; Frankiales; Frankiaceae; Frankia.
OX NCBI_TaxID=106370;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 45818 / CECT 9043 / CcI3;
RX PubMed=17151343; DOI=10.1101/gr.5798407;
RA Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N.,
RA Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A.,
RA Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P.,
RA Goltsman E., Huang Y., Kopp O.R., Labarre L., Lapidus A., Lavire C.,
RA Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J.,
RA Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F.,
RA Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C.,
RA Benson D.R.;
RT "Genome characteristics of facultatively symbiotic Frankia sp. strains
RT reflect host range and host plant biogeography.";
RL Genome Res. 17:7-15(2007).
CC -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC and one molecule of ATP is hydrogenolyzed for each amidation.
CC {ECO:0000255|HAMAP-Rule:MF_00028}.
CC -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC {ECO:0000255|HAMAP-Rule:MF_00028}.
CC -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR EMBL; CP000249; ABD11965.1; -; Genomic_DNA.
DR RefSeq; WP_011437000.1; NZ_JENI01000015.1.
DR AlphaFoldDB; Q2J9S7; -.
DR SMR; Q2J9S7; -.
DR STRING; 106370.Francci3_2603; -.
DR EnsemblBacteria; ABD11965; ABD11965; Francci3_2603.
DR KEGG; fra:Francci3_2603; -.
DR eggNOG; COG1492; Bacteria.
DR HOGENOM; CLU_019250_2_2_11; -.
DR OMA; EIHHGVA; -.
DR OrthoDB; 744477at2; -.
DR PhylomeDB; Q2J9S7; -.
DR UniPathway; UPA00148; -.
DR Proteomes; UP000001937; Chromosome.
DR GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR CDD; cd01750; GATase1_CobQ; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR HAMAP; MF_00028; CobQ; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR InterPro; IPR033949; CobQ_GATase1.
DR InterPro; IPR004459; CobQ_synth.
DR InterPro; IPR011698; GATase_3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR Pfam; PF01656; CbiA; 1.
DR Pfam; PF07685; GATase_3; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51274; GATASE_COBBQ; 1.
PE 3: Inferred from homology;
KW Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT CHAIN 1..520
FT /note="Cobyric acid synthase"
FT /id="PRO_1000002357"
FT DOMAIN 275..453
FT /note="GATase cobBQ-type"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT ACT_SITE 356
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT ACT_SITE 445
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ SEQUENCE 520 AA; 54677 MW; CED7BE094CA4E078 CRC64;
MSGGLLVAGT ASDAGKSVLT AGICRWLARE GVRVAPFKAQ NMALNSAVTA DGAEIGRAQA
MQAAAAGVEP EAAMNPVLLK PGGQRHSQLV VLGRPVAEVD ALGYRPYKER LAAIVLECLD
DLRGRFDAVI CEGAGSPAEI NLRSTDIANM GLARAANLPV IVVGDIDKGG VFAALFGTLA
LLDAADQALV AGWVINRFRG DARLLEPGLR QIERLTGRPV HGVVPWKAGL WLDVEDSLDL
AAFPDAEPCP DAEPCPDAEP CPEARPASHG GRREVLRVAV IRLPRLSNVT DIDALRVEPG
VAVRLATRPD ELADADLVIL PGTRSTVEDL RWLRRRGLAA ALAERAAAAR PVLGICGGYQ
ILGRRIRDDV ESGAGEVDGL GLLPVITTFD PVKLLGRRAA TDAAGRPLTG YEIRHGRLTV
EEHPDSAPFA ADGVRVGAVA GTSWHGVLEN DAFRRAYLAD VATAAGRSFV PAFTCFADAR
QRRLDALGDL VADHLDTGAL RRLLAEGTPA GLPFVPPGAS