ID   COBQ_LISMO              Reviewed;         511 AA.
AC   Q8Y7R3;
DT   25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Cobyric acid synthase {ECO:0000255|HAMAP-Rule:MF_00028};
GN   Name=cobQ {ECO:0000255|HAMAP-Rule:MF_00028}; Synonyms=cbiP;
GN   OrderedLocusNames=lmo1208;
OS   Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX   NCBI_TaxID=169963;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-679 / EGD-e;
RX   PubMed=11679669; DOI=10.1126/science.1063447;
RA   Glaser P., Frangeul L., Buchrieser C., Rusniok C., Amend A., Baquero F.,
RA   Berche P., Bloecker H., Brandt P., Chakraborty T., Charbit A.,
RA   Chetouani F., Couve E., de Daruvar A., Dehoux P., Domann E.,
RA   Dominguez-Bernal G., Duchaud E., Durant L., Dussurget O., Entian K.-D.,
RA   Fsihi H., Garcia-del Portillo F., Garrido P., Gautier L., Goebel W.,
RA   Gomez-Lopez N., Hain T., Hauf J., Jackson D., Jones L.-M., Kaerst U.,
RA   Kreft J., Kuhn M., Kunst F., Kurapkat G., Madueno E., Maitournam A.,
RA   Mata Vicente J., Ng E., Nedjari H., Nordsiek G., Novella S., de Pablos B.,
RA   Perez-Diaz J.-C., Purcell R., Remmel B., Rose M., Schlueter T., Simoes N.,
RA   Tierrez A., Vazquez-Boland J.-A., Voss H., Wehland J., Cossart P.;
RT   "Comparative genomics of Listeria species.";
RL   Science 294:849-852(2001).
CC   -!- FUNCTION: Catalyzes amidations at positions B, D, E, and G on
CC       adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine,
CC       and one molecule of ATP is hydrogenolyzed for each amidation.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
CC   -!- SIMILARITY: Belongs to the CobB/CobQ family. CobQ subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_00028}.
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DR   EMBL; AL591978; CAC99286.1; -; Genomic_DNA.
DR   PIR; AH1225; AH1225.
DR   RefSeq; NP_464733.1; NC_003210.1.
DR   RefSeq; WP_010989707.1; NZ_CP023861.1.
DR   AlphaFoldDB; Q8Y7R3; -.
DR   SMR; Q8Y7R3; -.
DR   STRING; 169963.lmo1208; -.
DR   PaxDb; Q8Y7R3; -.
DR   EnsemblBacteria; CAC99286; CAC99286; CAC99286.
DR   GeneID; 986064; -.
DR   KEGG; lmo:lmo1208; -.
DR   PATRIC; fig|169963.11.peg.1239; -.
DR   eggNOG; COG1492; Bacteria.
DR   HOGENOM; CLU_019250_2_2_9; -.
DR   OMA; EIHHGVA; -.
DR   PhylomeDB; Q8Y7R3; -.
DR   BioCyc; LMON169963:LMO1208-MON; -.
DR   UniPathway; UPA00148; -.
DR   Proteomes; UP000000817; Chromosome.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   CDD; cd01750; GATase1_CobQ; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00028; CobQ; 1.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR033949; CobQ_GATase1.
DR   InterPro; IPR004459; CobQ_synth.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR21343:SF1; PTHR21343:SF1; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00313; cobQ; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Glutamine amidotransferase; Reference proteome.
FT   CHAIN           1..511
FT                   /note="Cobyric acid synthase"
FT                   /id="PRO_0000141308"
FT   DOMAIN          251..443
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        332
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
FT   ACT_SITE        435
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00028"
SQ   SEQUENCE   511 AA;  56263 MW;  7947A5F27A02F278 CRC64;
     MVKQIMIQGT ASDAGKSVLV AGLCRLFKNK GKRVVPFKSQ NMSLNSFITA TGDEMGRAQV
     FQAEAAGVFP DVRMNPVLLK PTNDRQSQVI FMGAILDNMD AVTYHDFKQT LIPKIQAVYQ
     SLADENDIIV LEGAGSPAEI NLNDRDIVNM GMAKMVDAPV VLVADIDKGG VFASIYGTIM
     LLNEEERARI KGVIINKFRG DVALLQPGID MIEELTNVPV IGVIPYANLQ LEEEDSVSLN
     GKNYAPDSNA LLDIAIICLP RISNFTDFHS LEIQPEISLR YIRNLADFGK PDLVIIPGSK
     NTLEDMAFLE ESGLKKAIQN FAENAGKVIG ICGGYQMLGQ KMLDPDQVES KQLEIAGLGL
     LDTETIFLDQ KRTTQITGVT HSGEAVEGYE IHMGETKRGE STSPFCKIKA VNGNEETHQD
     GAISANKNII GTYIHGIFDN DVFLGNLFDE LLTRKNQSIY PHEIINLKEH KEQEYDKLAA
     LLEANIQMDQ LEKIMKGEKI CVSTQKPAIK E